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Gene Review

CLNS1A  -  chloride channel, nucleotide-sensitive, 1A

Homo sapiens

Synonyms: CLCI, CLNS1B, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, ...
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Disease relevance of CLNS1A

  • Glucose induces anion conductance and cytosol-to-membrane transposition of ICln in INS-1E rat insulinoma cells [1].

High impact information on CLNS1A


Biological context of CLNS1A


Anatomical context of CLNS1A

  • Immunocytochemistry experiments showed the presence of ICln protein in the cytosol and in the plasma membrane [8].
  • Thus, it is unlikely that pICln forms the channel that is responsible for the ICln current in Xenopus oocytes [9].
  • Experiments designed to knock down the ICln protein in NIH 3T3 fibroblasts as well as in epithelial cells led to the conclusion that this protein is crucially involved in volume regulation after cytoplasmic swelling [10].
  • A unique feature of ICln is the distinct sensitivity of these channels for nucleotides and nucleoside analogues added to the extracellular fluid [6].
  • We investigated the swelling-induced chloride current in fibroblasts, which we demonstrated is closely related or identical to a cloned epithelial chloride channel, ICln: This chloride channel can be blocked by nucleotides [11].

Associations of CLNS1A with chemical compounds

  • In parallel, a cell-permeable peptide corresponding to the potential integrin-recognition domain on ICln (AKFEEE, 10-100 microm) also inhibits platelet function [3].
  • pICln is a protein that induces an outwardly rectifying, nucleotide-sensitive chloride current (ICln) when expressed in Xenopus oocytes, but its precise function (plasma-membrane anion channel versus cytosolic regulator of a channel) remains controversial [9].
  • Outward rectification and the discrimination between NO3- and Cl- were more pronounced for ICln [7].
  • 5. The binding site for the two phenol derivatives onto ICln seems to be distinct but closely related to the nucleotide binding site identified as G x G x G, a glycine repeat located at the predicted outer mouth of the ICln channel protein [12].

Other interactions of CLNS1A

  • Characterization of the human gene coding for the swelling-dependent chloride channel ICln at position 11q13.5-14.1 (CLNS1A) and further characterization of the chromosome 6 (CLNS1B) localization [13].
  • However, other possible roles for ICln in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins [14].
  • By using full-length HRpICln cDNA (approx. 1.2 kb) to probe human lymphocyte metaphase-chromosome spreads, the location of the human ICln gene was mapped to 11q13 by fluorescence in situ hybridization analysis [15].

Analytical, diagnostic and therapeutic context of CLNS1A


  1. Glucose induces anion conductance and cytosol-to-membrane transposition of ICln in INS-1E rat insulinoma cells. Jakab, M., Grundbichler, M., Benicky, J., Ravasio, A., Chwatal, S., Schmidt, S., Strbak, V., Fürst, J., Paulmichl, M., Ritter, M. Cell. Physiol. Biochem. (2006) [Pubmed]
  2. ICln159 folds into a pleckstrin homology domain-like structure. Interaction with kinases and the splicing factor LSm4. Fürst, J., Schedlbauer, A., Gandini, R., Garavaglia, M.L., Saino, S., Gschwentner, M., Sarg, B., Lindner, H., Jakab, M., Ritter, M., Bazzini, C., Botta, G., Meyer, G., Kontaxis, G., Tilly, B.C., Konrat, R., Paulmichl, M. J. Biol. Chem. (2005) [Pubmed]
  3. ICln, a novel integrin alphaIIbbeta3-associated protein, functionally regulates platelet activation. Larkin, D., Murphy, D., Reilly, D.F., Cahill, M., Sattler, E., Harriott, P., Cahill, D.J., Moran, N. J. Biol. Chem. (2004) [Pubmed]
  4. ICln is essential for cellular and early embryonic viability. Pu, W.T., Wickman, K., Clapham, D.E. J. Biol. Chem. (2000) [Pubmed]
  5. Chromosomal localization of the genes (CLNS1A and CLNS1B) coding for the swelling-dependent chloride channel ICln. Nagl, U.O., Erdel, M., Schmarda, A., Seri, M., Pinggera, G.M., Gschwentner, M., Duba, C., Galietta, L.J., Deetjen, P., Utermann, G., Paulmichl, M. Genomics (1996) [Pubmed]
  6. ICln: a chloride channel paramount for cell volume regulation. Gschwentner, M., Susanna, A., Schmarda, A., Laich, A., Nagl, U.O., Ellemunter, H., Deetjen, P., Frick, J., Paulmichl, M. J. Allergy Clin. Immunol. (1996) [Pubmed]
  7. The chloride current induced by expression of the protein pICln in Xenopus oocytes differs from the endogenous volume-sensitive chloride current. Voets, T., Buyse, G., Tytgat, J., Droogmans, G., Eggermont, J., Nilius, B. J. Physiol. (Lond.) (1996) [Pubmed]
  8. Volume-regulated Cl- channels in human pleural mesothelioma cells. Meyer, G., Rodighiero, S., Guizzardi, F., Bazzini, C., Bottà, G., Bertocchi, C., Garavaglia, L., Dossena, S., Manfredi, R., Sironi, C., Catania, A., Paulmichl, M. FEBS Lett. (2004) [Pubmed]
  9. Expression of human pICln and ClC-6 in Xenopus oocytes induces an identical endogenous chloride conductance. Buyse, G., Voets, T., Tytgat, J., De Greef, C., Droogmans, G., Nilius, B., Eggermont, J. J. Biol. Chem. (1997) [Pubmed]
  10. The promoter for constitutive expression of the human ICln gene CLNS1A. Scandella, E., Nagl, U.O., Oehl, B., Bergmann, F., Gschwentner, M., Fürst, J., Schmarda, A., Ritter, M., Waldegger, S., Lang, F., Deetjen, P., Paulmichl, M. J. Biol. Chem. (2000) [Pubmed]
  11. Antiviral drugs from the nucleoside analog family block volume-activated chloride channels. Gschwentner, M., Susanna, A., Wöll, E., Ritter, M., Nagl, U.O., Schmarda, A., Laich, A., Pinggera, G.M., Ellemunter, H., Huemer, H. Mol. Med. (1995) [Pubmed]
  12. Blockade of swelling-induced chloride channels by phenol derivatives. Gschwentner, M., Jungwirth, A., Hofer, S., Wöll, E., Ritter, M., Susanna, A., Schmarda, A., Reibnegger, G., Pinggera, G.M., Leitinger, M., Frick, J., Deetjen, P., Paulmichl, M. Br. J. Pharmacol. (1996) [Pubmed]
  13. Characterization of the human gene coding for the swelling-dependent chloride channel ICln at position 11q13.5-14.1 (CLNS1A) and further characterization of the chromosome 6 (CLNS1B) localization. Nagl, U.O., Erdel, M., Bergmann, F., Oehl, B., Scandella, E., Musante, L., Galietta, L.J., Gschwentner, M., Fürst, J., Schmarda, A., Hofer, S., Utermann, G., Deetjen, P., Paulmichl, M. Gene (1998) [Pubmed]
  14. Determination of protein-protein interactions of ICIn by the yeast two-hybrid system. Schmarda, A., Fresser, F., Gschwentner, M., Fürst, J., Ritter, M., Lang, F., Baier, G., Paulmichl, M. Cell. Physiol. Biochem. (2001) [Pubmed]
  15. Molecular cloning and expression of a chloride channel-associated protein pICln in human young red blood cells: association with actin. Schwartz, R.S., Rybicki, A.C., Nagel, R.L. Biochem. J. (1997) [Pubmed]
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