The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

HIS6  -  1-(5-phosphoribosyl)-5- ((5...

Saccharomyces cerevisiae S288c

Synonyms: 5-proFAR isomerase, Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, YIL020C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of HIS6


High impact information on HIS6


Biological context of HIS6


Anatomical context of HIS6

  • A V5 epitope and His6-tagged version of Ynl275wp was expressed in yeast and was localized to the plasma membrane by subcellular fractionation and immunofluorescence labeling [12].
  • In comparison N-glycosylated hisGal-T transiently expressed in COS cells was secreted as a soluble active enzyme and could be purified in one single step by virtue of the His6-tag [13].
  • One library was derived from mice immunized with recombinant CDC2aAt N-terminally fused to a His6-tag (His-CDC2aAt) and the other was made out of an anti-PSTAIRE hybridoma cell line [14].

Associations of HIS6 with chemical compounds

  • Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes [1].
  • STP1-His6 protein is functionally active after solubilization with octyl-beta-D-glucoside, which was shown by insertion of the protein into proteoliposomes by detergent dilution and determination of the resulting transport capacity [9].
  • The intrinsic fluorescence of two CCP derivatives with the quencher pentaammineruthenium(III) covalently attached to His6 and His60 has also been characterized [15].
  • A genetically modified (His6-tagged) form of the mitochondrial F1-ATPase (MW = 370 kDa) has been purified from the yeast Saccharomyces cerevisiae and crystallized in the presence of polyethelene glycol (PEG) 6000 as a precipitant, 1 mM NiCl2, 1 mM Mg AMP-PNP and 50 microM Mg ADP [16].

Regulatory relationships of HIS6


Analytical, diagnostic and therapeutic context of HIS6

  • Plc1p, modified by in-frame insertion of a His6 tract and a c-myc epitope near its amino terminus, was overexpressed from the GAL1 promoter, partially purified by nickel chelate affinity chromatography, and shown to be an active PLC enzyme in vitro with properties similar to those of its mammalian counterparts [18].
  • This protein-protein interaction has been validated by immunoprecipitation in both COS-1 and Huh7 cells and by His6 pull-down assays [19].
  • X-ray crystallography showed that a5Ru is coordinated to His60 in one derivative [Fox et al. (1990) J. Am. Chem. Soc. 112, 7426]; HPLC and mass spectral analysis of the tryptic peptides of the other derivative identified a peptide (MW = 1469 Da) corresponding to residues 1-12 of CCP plus a5Ru, indicating His6 as the site of modification [20].


  1. Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes. Fani, R., Tamburini, E., Mori, E., Lazcano, A., Liò, P., Barberio, C., Casalone, E., Cavalieri, D., Perito, B., Polsinelli, M. Gene (1997) [Pubmed]
  2. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane. Armstrong, L.C., Saenz, A.J., Bornstein, P. J. Cell. Biochem. (1999) [Pubmed]
  3. Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae. Meeks-Wagner, D., Wood, J.S., Garvik, B., Hartwell, L.H. Cell (1986) [Pubmed]
  4. Brefeldin A-inhibited guanine nucleotide-exchange activity of Sec7 domain from yeast Sec7 with yeast and mammalian ADP ribosylation factors. Sata, M., Donaldson, J.G., Moss, J., Vaughan, M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  5. RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. Kolodziej, P., Young, R.A. Mol. Cell. Biol. (1989) [Pubmed]
  6. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Chan, R.K., Otte, C.A. Mol. Cell. Biol. (1982) [Pubmed]
  7. The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant. Beeler, T., Bacikova, D., Gable, K., Hopkins, L., Johnson, C., Slife, H., Dunn, T. J. Biol. Chem. (1998) [Pubmed]
  8. Vectors for expressing T7 epitope- and His6 affinity-tagged fusion proteins in S. cerevisiae. Enomoto, S., Chen, G., Berman, J. BioTechniques (1998) [Pubmed]
  9. Functional reconstitution of the solubilized Arabidopsis thaliana STP1 monosaccharide-H+ symporter in lipid vesicles and purification of the histidine tagged protein from transgenic Saccharomyces cerevisiae. Stolz, J., Stadler, R., Opekarová, M., Sauer, N. Plant J. (1994) [Pubmed]
  10. spp42, identified as a classical suppressor of prp4-73, which encodes a kinase involved in pre-mRNA splicing in fission yeast, is a homologue of the splicing factor Prp8p. Schmidt, H., Richert, K., Drakas, R.A., Käufer, N.F. Genetics (1999) [Pubmed]
  11. Secondary-structure characterization by far-UV CD of highly purified uncoupling protein 1 expressed in yeast. Douette, P., Navet, R., Bouillenne, F., Brans, A., Sluse-Goffart, C., Matagne, A., Sluse, F.E. Biochem. J. (2004) [Pubmed]
  12. Expression and characterization of the anion transporter homologue YNL275w in Saccharomyces cerevisiae. Zhao, R., Reithmeier, R.A. Am. J. Physiol., Cell Physiol. (2001) [Pubmed]
  13. Expression and purification of His-tagged beta-1,4-galactosyltransferase in yeast and in COS cells. Borsig, L., Berger, E.G., Malissard, M. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  14. Isolation and characterization of recombinant antibody fragments against CDC2a from Arabidopsis thaliana. Eeckhout, D., Fiers, E., Sienaert, R., Snoeck, V., Depicker, A., De Jaeger, G. Eur. J. Biochem. (2000) [Pubmed]
  15. Quenching of intrinsic fluorescence of yeast cytochrome c peroxidase by covalently- and noncovalently-bound quenchers. Fox, T., Ferreira-Rajabi, L., Hill, B.C., English, A.M. Biochemistry (1993) [Pubmed]
  16. Crystallization and preliminary crystallographic studies of the mitochondrial F1-ATPase from the yeast Saccharomyces cerevisiae. Mueller, D.M., Puri, N., Kabaleeswaran, V., Terry, C., Leslie, A.G., Walker, J.E. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  17. Crystallization and preliminary X-ray diffraction analysis of NADPH-dependent thioredoxin reductase I from Saccharomyces cerevisiae. Oliveira, M.A., Discola, K.F., Alves, S.V., Barbosa, J.A., Medrano, F.J., Netto, L.E., Guimarães, B.G. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2005) [Pubmed]
  18. Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae. Flick, J.S., Thorner, J. Mol. Cell. Biol. (1993) [Pubmed]
  19. The 41-amino-acid C-terminal region of the hepatitis E virus ORF3 protein interacts with bikunin, a kunitz-type serine protease inhibitor. Tyagi, S., Surjit, M., Lal, S.K. J. Virol. (2005) [Pubmed]
  20. Derivatization of yeast cytochrome c peroxidase with pentaammineruthenium(III). Fox, T., English, A.M., Gibbs, B.F. Bioconjug. Chem. (1994) [Pubmed]
WikiGenes - Universities