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Gene Review

FAS2  -  trifunctional fatty acid synthase subunit...

Saccharomyces cerevisiae S288c

Synonyms: Fatty acid synthase subunit alpha, P1409, YPL231W
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Disease relevance of FAS2


High impact information on FAS2

  • Balanced levels of subunits alpha and beta are ensured by an autoregulatory effect of FAS1 on FAS2 expression and by posttranslational degradation of excess FAS subunits [3].
  • In this study, we present the nucleotide sequence of the FAS2 gene [4].
  • We could not find any sequence homology in the 5' flanking sequence of the FAS1 and FAS2 genes that would suggest common regulatory function [4].
  • Regulatory gene INO4 of yeast phospholipid biosynthesis is positively autoregulated and functions as a transactivator of fatty acid synthase genes FAS1 and FAS2 from Saccharomyces cerevisiae [5].
  • The role of ACP1 in long-chain fatty acid synthesis was studied in fast and fas2 null mutants completely lacking cytoplasmic fatty acid synthase [6].

Biological context of FAS2

  • These data suggest an activating role of subunit beta on FAS2 gene expression or, alternatively, a repression of FAS2 by an excess of its own gene product [7].
  • FAS2-lacZ fusion constructs deleted for this region showed high reporter gene expression even in the absence of FAS1, arguing for a negatively-acting downstream repression site (DRS) responsible for FAS1-dependent expression of FAS2 [8].
  • Deletion analyses of the regulatory regions of FAS1 and FAS2 revealed common regulatory sequences [9].
  • These experiments identify the yeast DNA sequences cloned into 33F1 as originating from the FAS1 gene and those DNA sequences in 102B5, from the FAS2 gene [10].
  • Mapping of the trifunctional fatty acid synthetase gene FAS2 on chromosome XVI of Saccharomyces cerevisiae [11].

Associations of FAS2 with chemical compounds

  • Previous studies have shown that the genes coding for fatty acid synthase, FAS1 and FAS2, are regulated by inositol (Chirala, S.S. [1992] Proc. Natl. Acad. Sci. USA 89, 10232-10236) [12].
  • Expression of the unlinked genes, FAS1 and FAS2, is in part constitutive and in part subject to repression by the phospholipid precursors inositol and choline [3].
  • In addition, no differences were noted when comparing the FAS2 sequence, that encompasses the cerulenin-binding domain of FAS, between strain 4918 and two derived cerulenin-resistant (CerR) mutants [13].
  • This growth inhibition allowed efficient counter-selection for cells that had undergone homologous recombination between the FAS2 repeats by their growth on galactose medium [14].
  • Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sake [15].

Physical interactions of FAS2


Other interactions of FAS2

  • Compared to the intact alpha 6 beta 6 complex, the individual FAS subunits synthesized in the delta fas1 or delta fas2 strains exhibit a considerably increased sensitivity towards the proteinases present in the yeast cell homogenate [7].
  • The overexpression of FAS1 considerably stimulated MCFA formation while that of ASC2, ACC1 and FAS2 genes was not effective [17].
  • However, in contrast to B. ammoniagenes PPT1, this sequence is an integral part of the yeast FAS2 gene [18].

Analytical, diagnostic and therapeutic context of FAS2


  1. Molecular cloning of the yeast fatty acid synthetase genes, FAS1 and FAS2: illustrating the structure of the FAS1 cluster gene by transcript mapping and transformation studies. Schweizer, M., Lebert, C., Höltke, J., Roberts, L.M., Schweizer, E. Mol. Gen. Genet. (1984) [Pubmed]
  2. Molecular structure of the multifunctional fatty acid synthetase gene of Brevibacterium ammoniagenes: its sequence of catalytic domains is formally consistent with a head-to-tail fusion of the two yeast genes FAS1 and FAS2. Meurer, G., Biermann, G., Schütz, A., Harth, S., Schweizer, E. Mol. Gen. Genet. (1992) [Pubmed]
  3. Microbial type I fatty acid synthases (FAS): major players in a network of cellular FAS systems. Schweizer, E., Hofmann, J. Microbiol. Mol. Biol. Rev. (2004) [Pubmed]
  4. Primary structure of the multifunctional alpha subunit protein of yeast fatty acid synthase derived from FAS2 gene sequence. Mohamed, A.H., Chirala, S.S., Mody, N.H., Huang, W.Y., Wakil, S.J. J. Biol. Chem. (1988) [Pubmed]
  5. Regulatory gene INO4 of yeast phospholipid biosynthesis is positively autoregulated and functions as a transactivator of fatty acid synthase genes FAS1 and FAS2 from Saccharomyces cerevisiae. Schüller, H.J., Schorr, R., Hoffmann, B., Schweizer, E. Nucleic Acids Res. (1992) [Pubmed]
  6. Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae. Brody, S., Oh, C., Hoja, U., Schweizer, E. FEBS Lett. (1997) [Pubmed]
  7. Differential proteolytic sensitivity of yeast fatty acid synthetase subunits alpha and beta contributing to a balanced ratio of both fatty acid synthetase components. Schüller, H.J., Förtsch, B., Rautenstrauss, B., Wolf, D.H., Schweizer, E. Eur. J. Biochem. (1992) [Pubmed]
  8. A downstream regulatory element located within the coding sequence mediates autoregulated expression of the yeast fatty acid synthase gene FAS2 by the FAS1 gene product. Wenz, P., Schwank, S., Hoja, U., Schüller, H.J. Nucleic Acids Res. (2001) [Pubmed]
  9. Coordinated regulation and inositol-mediated and fatty acid-mediated repression of fatty acid synthase genes in Saccharomyces cerevisiae. Chirala, S.S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  10. Molecular cloning of fatty acid synthetase genes from Saccharomyces cerevisiae. Kuziora, M.A., Chalmers, J.H., Douglas, M.G., Hitzeman, R.A., Mattick, J.S., Wakil, S.J. J. Biol. Chem. (1983) [Pubmed]
  11. Mapping of the trifunctional fatty acid synthetase gene FAS2 on chromosome XVI of Saccharomyces cerevisiae. Siebenlist, U., Nix, J., Schweizer, M., Jäger, D., Schweizer, E. Yeast (1990) [Pubmed]
  12. Analysis of FAS3/ACC regulatory region of Saccharomyces cerevisiae: identification of a functional UASINO and sequences responsible for fatty acid mediated repression. Chirala, S.S., Zhong, Q., Huang, W., al-Feel, W. Nucleic Acids Res. (1994) [Pubmed]
  13. Analysis and expression of the Candida albicans FAS2 gene. Southard, S.B., Cihlar, R.L. Gene (1995) [Pubmed]
  14. Construction of recombinant sake yeast containing a dominant FAS2 mutation without extraneous sequences by a two-step gene replacement protocol. Akada, R., Matsuo, K., Aritomi, K., Nishizawa, Y. J. Biosci. Bioeng. (1999) [Pubmed]
  15. Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sake. Aritomi, K., Hirosawa, I., Hoshida, H., Shiigi, M., Nishizawa, Y., Kashiwagi, S., Akada, R. Biosci. Biotechnol. Biochem. (2004) [Pubmed]
  16. Importance of general regulatory factors Rap1p, Abf1p and Reb1p for the activation of yeast fatty acid synthase genes FAS1 and FAS2. Schüller, H.J., Schütz, A., Knab, S., Hoffmann, B., Schweizer, E. Eur. J. Biochem. (1994) [Pubmed]
  17. Increased ethyl caproate production by inositol limitation in Saccharomyces cerevisiae. Furukawa, K., Yamada, T., Mizoguchi, H., Hara, S. J. Biosci. Bioeng. (2003) [Pubmed]
  18. Identification, isolation and biochemical characterization of a phosphopantetheine:protein transferase that activates the two type-I fatty acid synthases of Brevibacterium ammoniagenes. Stuible, H.P., Meier, S., Schweizer, E. Eur. J. Biochem. (1997) [Pubmed]
  19. Cerulenin-resistant mutants of Saccharomyces cerevisiae with an altered fatty acid synthase gene. Inokoshi, J., Tomoda, H., Hashimoto, H., Watanabe, A., Takeshima, H., Omura, S. Mol. Gen. Genet. (1994) [Pubmed]
  20. Detection of a point mutation in FAS2 gene of sake yeast strains by allele-specific PCR amplification. Akada, R., Hirosawa, I., Hoshida, H., Nishizawa, Y. J. Biosci. Bioeng. (2001) [Pubmed]
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