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Gene Review

TAF1B  -  TATA box binding protein (TBP)-associated...

Homo sapiens

Synonyms: MGC:9349, RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, ...
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Disease relevance of TAF1B

  • The very high frequency of mutations, biallelic mutations and the predicted truncation of protein products suggests that mutations of MARCKS, FLJ11383 and TAF1B are selected, and play a role in the tumorigenesis of MSI-H colorectal carcinomas [1].
  • Pertussis toxin inhibited the ability of SL-1 to both stimulate neutrophils directly and to prime neutrophils for subsequent responses induced by PMA, suggesting a role for one or more guanine nucleotide regulating proteins in both responses [2].
  • The principal sulfatide of a group of acidic lipids from virulent Mycobacterium tuberculosis, sulfolipid-1 (SL-1), stimulates neutrophil superoxide (O2-) generation and, at lower concentrations, primes neutrophil response to several other metabolic agonists including FMLP, and PMA [2].
  • Human immunodeficiency virus type 1 (HIV-1) genomic RNA segments at nucleotide (nt) positions +240 to +274 are thought to form a stem-loop secondary structure, termed SL1, that serves as a dimerization initiation site for viral genomic RNA [3].
  • The role of the SL1 stem in heterozygous virion formation was also tested; our results indicated that the intermolecular base pairing of the stem sequences does not affect RNA partner selection [4].

High impact information on TAF1B

  • DNAase footprinting revealed that although SL1 alone does not bind specifically to rRNA promoter sequences, a second factor, UBF1, recruits SL1 to the template and directs binding to an extended region encompassing sequences in the UCE [5].
  • Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively [6].
  • These results strongly suggest an important role of transcription activation domains of hUBF in mediating interactions with the TBP-TAF complex hSL1 [7].
  • The presence of c-Myc at specific sites on rDNA coincides with the recruitment of SL1 to the rDNA promoter and with increased histone acetylation [8].
  • In mammalian RNA polymerase I transcription, SL1, an assembly of TBP and associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene promoters in vitro [9].

Biological context of TAF1B

  • Here, we map the functional domains of the nucleolar HMG box protein hUBF, which binds to the human rRNA promoter and stimulates transcription by RNA polymerase I through cooperative interactions with a distinct TBP-TAF complex, hSL1 [7].
  • Recruitment of TATA-binding protein-TAFI complex SL1 to the human ribosomal DNA promoter is mediated by the carboxy-terminal activation domain of upstream binding factor (UBF) and is regulated by UBF phosphorylation [10].
  • Substitution of three nucleotides in the SL1 loop (SL1A3) abolishes SMN interaction, and the corresponding U1 snRNA (U1A3) is impaired in U1 snRNP biogenesis [11].
  • However, a direct role for SL-1 in M. tuberculosis virulence has not been established [12].
  • These responses to SL-1 were examined in relation to diacylglycerol (DAG) generation, Ca2+ availability and activation of guanine nucleotide binding proteins to clarify the signal transduction pathways involved [2].

Anatomical context of TAF1B


Associations of TAF1B with chemical compounds

  • Depletion of extracellular Ca2+ ablated O2- release induced by stimulatory levels of SL-1 but did not inhibit the priming effect induced by substimulatory concentrations of the lipid [2].
  • The putative zinc finger has cysteine residues with identical spacing and a similar amino acid composition to those found in the species-specific transcription initiation factors SL1 and TIF-IB [14].
  • Irreversible inhibition of soybean lipoxygenase-1 (SL-1) was accomplished via a controlled potential oxidative electrolysis of 1,5-dihydroxynaphthalene (1,5-DHN) at +0.8 V vs SCE [15].
  • First, we treated all groups A and B patients 3 times, 20 min each time, with a Minilith SL1, and then only the patients of the second group received a complete cycle of twelve injections of verapamil (10 mg) every 2 weeks for 6 months [16].

Other interactions of TAF1B

  • We have determined that the human genes TAF1A, TAF1B and TAF1C, encoding these three TAF(I) polypeptides, are localized at lq42, 2p25 and 16q24, respectively [13].

Analytical, diagnostic and therapeutic context of TAF1B

  • However, chromatin immunoprecipitation assays demonstrate that PTEN differentially reduces the occupancy of the SL1 subunits on the rRNA gene promoter [17].
  • Test of these mutations in our biological assay revealed that only stem loop 1 (SL1) was important for the packaging potential of MPMV, while mutations in none of the other stem loops affected packaging significantly [18].
  • Enterobius vermicularis: specific detection by amplification of an internal region of 5S ribosomal RNA intergenic spacer and trans-splicing leader RNA analysis. E. vermicularis: specific detection by PCR and SL1 RNA analysis [19].


  1. Identification of MARCKS, FLJ11383 and TAF1B as putative novel target genes in colorectal carcinomas with microsatellite instability. Kim, N.G., Rhee, H., Li, L.S., Kim, H., Lee, J.S., Kim, J.H., Kim, N.K., Kim, H. Oncogene (2002) [Pubmed]
  2. Activation of human neutrophils by Mycobacterium tuberculosis-derived sulfolipid-1. Zhang, L., English, D., Andersen, B.R. J. Immunol. (1991) [Pubmed]
  3. Mutations within four distinct gag proteins are required to restore replication of human immunodeficiency virus type 1 after deletion mutagenesis within the dimerization initiation site. Liang, C., Rong, L., Quan, Y., Laughrea, M., Kleiman, L., Wainberg, M.A. J. Virol. (1999) [Pubmed]
  4. Dimer Initiation Signal of Human Immunodeficiency Virus Type 1: Its Role in Partner Selection during RNA Copackaging and Its Effects on Recombination. Moore, M.D., Fu, W., Nikolaitchik, O., Chen, J., Ptak, R.G., Hu, W.S. J. Virol. (2007) [Pubmed]
  5. Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control element. Learned, R.M., Learned, T.K., Haltiner, M.M., Tjian, R.T. Cell (1986) [Pubmed]
  6. A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III. Henry, R.W., Sadowski, C.L., Kobayashi, R., Hernandez, N. Nature (1995) [Pubmed]
  7. Multiple domains of the RNA polymerase I activator hUBF interact with the TATA-binding protein complex hSL1 to mediate transcription. Jantzen, H.M., Chow, A.M., King, D.S., Tjian, R. Genes Dev. (1992) [Pubmed]
  8. c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. Grandori, C., Gomez-Roman, N., Felton-Edkins, Z.A., Ngouenet, C., Galloway, D.A., Eisenman, R.N., White, R.J. Nat. Cell Biol. (2005) [Pubmed]
  9. A novel TBP-associated factor of SL1 functions in RNA polymerase I transcription. Gorski, J.J., Pathak, S., Panov, K., Kasciukovic, T., Panova, T., Russell, J., Zomerdijk, J.C. EMBO J. (2007) [Pubmed]
  10. Recruitment of TATA-binding protein-TAFI complex SL1 to the human ribosomal DNA promoter is mediated by the carboxy-terminal activation domain of upstream binding factor (UBF) and is regulated by UBF phosphorylation. Tuan, J.C., Zhai, W., Comai, L. Mol. Cell. Biol. (1999) [Pubmed]
  11. Sequence-specific interaction of U1 snRNA with the SMN complex. Yong, J., Pellizzoni, L., Dreyfuss, G. EMBO J. (2002) [Pubmed]
  12. MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence. Converse, S.E., Mougous, J.D., Leavell, M.D., Leary, J.A., Bertozzi, C.R., Cox, J.S. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  13. Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1. Di Pietro, C., Rapisarda, A., Amico, V., Bonaiuto, C., Viola, A., Scalia, M., Motta, S., Amato, A., Engel, H., Messina, A., Sichel, G., Grzeschik, K., Purrello, M. Cytogenet. Cell Genet. (2000) [Pubmed]
  14. Determination of the entire sequence of turtle CR1: the first open reading frame of the turtle CR1 element encodes a protein with a novel zinc finger motif. Kajikawa, M., Ohshima, K., Okada, N. Mol. Biol. Evol. (1997) [Pubmed]
  15. Electrolysis-mediated irreversible inactivation of lipoxygenase directed toward electroaffinity labelling. Holmes, T.J., Vennerstrom, J.L., John, V. Biochem. Biophys. Res. Commun. (1984) [Pubmed]
  16. Our experience on the association of a new physical and medical therapy in patients suffering from induratio penis plastica. Mirone, V., Imbimbo, C., Palmieri, A., Fusco, F. Eur. Urol. (1999) [Pubmed]
  17. PTEN represses RNA Polymerase I transcription by disrupting the SL1 complex. Zhang, C., Comai, L., Johnson, D.L. Mol. Cell. Biol. (2005) [Pubmed]
  18. Mutational analysis of the predicted secondary RNA structure of the Mason-Pfizer monkey virus packaging signal. Mustafa, F., Lew, K.A., Schmidt, R.D., Browning, M.T., Rizvi, T.A. Virus Res. (2004) [Pubmed]
  19. Enterobius vermicularis: specific detection by amplification of an internal region of 5S ribosomal RNA intergenic spacer and trans-splicing leader RNA analysis. E. vermicularis: specific detection by PCR and SL1 RNA analysis. Iñiguez, A.M., Vicente, A.C., Araújo, A., Ferreira, L.F., Reinhard, K.J. Exp. Parasitol. (2002) [Pubmed]
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