Disease relevance of ftsI

• We have identified a gene involved in bacterial cell division, located immediately upstream of the ftsI gene in the min 2 region of the Escherichia coli chromosome [1].
• Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum [2].
• Clones containing the pbpB gene which encodes penicillin-binding protein (PBP) 3 of Pseudomonas aeruginosa were detected by hybridization by PCR amplification with primers based on the conserved sequences of high-molecular-weight PBPs [3].
• We have cloned and sequenced the promoter-proximal region of the Bacillus subtilis operon containing the pbpB gene, encoding essential penicillin-binding protein PBP2B [4].
• These phages were used to identify the previously undiscovered cell division gene sep [5].

High impact information on ftsI

• These multiple FtsZ rings were more apparent in longer cells; double or triple rings were often found in the nucleoid-free gaps in ftsI min and ftsA min double mutant filaments [6].
• Nucleotide sequence of the regulatory region of the gene pbpB of Escherichia coli [7].
• The context of this gene in the B. subtilis chromosome, immediately upstream of the mur operon, suggests that it is related to the pbpB gene of Escherichia coli, which is involved in the synthesis of septal peptidoglycan during cell division [8].
• The pbpB gene was located upstream of a gene homologous to the E. coli murE gene, which encodes uridine diphosphate-N-acetyl muramic acid-tripeptide synthetase [3].
• The modified ftsI genes were placed under the control of the fused lpp promoter and lac promoter/operator; expression of the truncated PBP3s was optimized by varying the copy number of the recombinant plasmids and the amount of LacI repressor, and export was facilitated by increasing the SecB content of the producing strain [9].

Chemical compound and disease context of ftsI

• Analysis of the length distribution of murein glycan strands in ftsZ and ftsI mutants of E. coli [10].

Biological context of ftsI

• The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs [11].
• In order to complement this null allele, the ftsI gene cloned on a single-copy mini-F plasmid required a region 1.9 kb upstream, which was found to contain a promoter sequence that could direct expression of a promoterless lacZ gene on a mini-F plasmid [12].
• For mutagenesis, the gene coding for PBP3 (ftsI) was inserted into the expression cloning vector pIN-IIB [13].
• We report that inactivation of the ftsI gene product, penicillin binding protein 3, by either beta-lactam antibiotics or genetic mutation induces SOS in Escherichia coli through the DpiBA two-component signal transduction system [14].
• A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli [15].

Anatomical context of ftsI

• Moreover, both pbpB genes are located in the same relative position within a cluster of cell division and cell wall genes on their respective chromosomes [16].

Associations of ftsI with chemical compounds

• Therefore, we investigated the response to the bacteriolytic beta-lactam cefsulodin of ftsZ and ftsI mutants growing at the restrictive (42 degrees C) temperature [17].
• In addition, FtsK-GFP localized to potential division sites in cephalexin-induced and ftsI mutant filaments, further supporting the idea that FtsK-GFP can target early, perhaps by recognizing FtsZ directly [18].
• This revealed a significant change in glycan chain lengths in newly synthesized murein associated with inactivation of the ftsZ gene product but not with inactivation of the ftsI gene product [10].

Other interactions of ftsI

• A shortened fragment in which the major portion of ftsI was deleted also had lethal effects on ftsQ and ftsZ mutants [19].
• A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW [12].
• Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutations [20].

Analytical, diagnostic and therapeutic context of ftsI

• We used PCR to randomly mutagenize ftsI, ligated the products into a green fluorescent protein fusion vector, and screened approximately 7,500 transformants for gfp-ftsI alleles that failed to complement an ftsI null mutant [21].
• The pbpB and ftsQ mutants also exhibited a prolonged duration of the constriction period [22].
• We developed a whole-cell bioluminescent biosensor, PpF1G4, which contains a chromosomally based sep-lux transcriptional fusion [23].

References