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Gene Review

ftsI  -  transpeptidase involved in septal...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0085, JW0082, pbpB, sep
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Disease relevance of ftsI


High impact information on ftsI

  • These multiple FtsZ rings were more apparent in longer cells; double or triple rings were often found in the nucleoid-free gaps in ftsI min and ftsA min double mutant filaments [6].
  • Nucleotide sequence of the regulatory region of the gene pbpB of Escherichia coli [7].
  • The context of this gene in the B. subtilis chromosome, immediately upstream of the mur operon, suggests that it is related to the pbpB gene of Escherichia coli, which is involved in the synthesis of septal peptidoglycan during cell division [8].
  • The pbpB gene was located upstream of a gene homologous to the E. coli murE gene, which encodes uridine diphosphate-N-acetyl muramic acid-tripeptide synthetase [3].
  • The modified ftsI genes were placed under the control of the fused lpp promoter and lac promoter/operator; expression of the truncated PBP3s was optimized by varying the copy number of the recombinant plasmids and the amount of LacI repressor, and export was facilitated by increasing the SecB content of the producing strain [9].

Chemical compound and disease context of ftsI


Biological context of ftsI


Anatomical context of ftsI


Associations of ftsI with chemical compounds

  • Therefore, we investigated the response to the bacteriolytic beta-lactam cefsulodin of ftsZ and ftsI mutants growing at the restrictive (42 degrees C) temperature [17].
  • In addition, FtsK-GFP localized to potential division sites in cephalexin-induced and ftsI mutant filaments, further supporting the idea that FtsK-GFP can target early, perhaps by recognizing FtsZ directly [18].
  • This revealed a significant change in glycan chain lengths in newly synthesized murein associated with inactivation of the ftsZ gene product but not with inactivation of the ftsI gene product [10].

Other interactions of ftsI


Analytical, diagnostic and therapeutic context of ftsI

  • We used PCR to randomly mutagenize ftsI, ligated the products into a green fluorescent protein fusion vector, and screened approximately 7,500 transformants for gfp-ftsI alleles that failed to complement an ftsI null mutant [21].
  • The pbpB and ftsQ mutants also exhibited a prolonged duration of the constriction period [22].
  • We developed a whole-cell bioluminescent biosensor, PpF1G4, which contains a chromosomally based sep-lux transcriptional fusion [23].


  1. FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli. Guzman, L.M., Barondess, J.J., Beckwith, J. J. Bacteriol. (1992) [Pubmed]
  2. Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum. Wijayarathna, C.D., Wachi, M., Nagai, K. Appl. Microbiol. Biotechnol. (2001) [Pubmed]
  3. Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3. Liao, X., Hancock, R.E. Antimicrob. Agents Chemother. (1995) [Pubmed]
  4. A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis. Daniel, R.A., Williams, A.M., Errington, J. J. Bacteriol. (1996) [Pubmed]
  5. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. Fletcher, G., Irwin, C.A., Henson, J.M., Fillingim, C., Malone, M.M., Walker, J.R. J. Bacteriol. (1978) [Pubmed]
  6. FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization. Yu, X.C., Margolin, W. Mol. Microbiol. (1999) [Pubmed]
  7. Nucleotide sequence of the regulatory region of the gene pbpB of Escherichia coli. Gómez, M.J., Fluoret, B., van Heijenoort, J., Ayala, J.A. Nucleic Acids Res. (1990) [Pubmed]
  8. The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis. Daniel, R.A., Drake, S., Buchanan, C.E., Scholle, R., Errington, J. J. Mol. Biol. (1994) [Pubmed]
  9. Engineering and overexpression of periplasmic forms of the penicillin-binding protein 3 of Escherichia coli. Fraipont, C., Adam, M., Nguyen-Distèche, M., Keck, W., Van Beeumen, J., Ayala, J.A., Granier, B., Hara, H., Ghuysen, J.M. Biochem. J. (1994) [Pubmed]
  10. Analysis of the length distribution of murein glycan strands in ftsZ and ftsI mutants of E. coli. Ishidate, K., Ursinus, A., Höltje, J.V., Rothfield, L. FEMS Microbiol. Lett. (1998) [Pubmed]
  11. Nucleotide sequence of the murE gene of Escherichia coli. Tao, J.S., Ishiguro, E.E. Can. J. Microbiol. (1989) [Pubmed]
  12. A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW. Hara, H., Yasuda, S., Horiuchi, K., Park, J.T. J. Bacteriol. (1997) [Pubmed]
  13. Binding of penicillin to thiol-penicillin-binding protein 3 of Escherichia coli: identification of its active site. Houba-Hérin, N., Hara, H., Inouye, M., Hirota, Y. Mol. Gen. Genet. (1985) [Pubmed]
  14. SOS response induction by beta-lactams and bacterial defense against antibiotic lethality. Miller, C., Thomsen, L.E., Gaggero, C., Mosseri, R., Ingmer, H., Cohen, S.N. Science (2004) [Pubmed]
  15. A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli. Ayala, J.A., Plá, J., Desviat, L.R., de Pedro, M.A. J. Bacteriol. (1988) [Pubmed]
  16. Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis. Yanouri, A., Daniel, R.A., Errington, J., Buchanan, C.E. J. Bacteriol. (1993) [Pubmed]
  17. Fast lysis of Escherichia coli filament cells requires differentiation of potential division sites. de Pedro, M.A., Höltje, J.V., Schwarz, H. Microbiology (Reading, Engl.) (2002) [Pubmed]
  18. Localization of cell division protein FtsK to the Escherichia coli septum and identification of a potential N-terminal targeting domain. Yu, X.C., Tran, A.H., Sun, Q., Margolin, W. J. Bacteriol. (1998) [Pubmed]
  19. Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes. Jung, H.K., Ishino, F., Matsuhashi, M. J. Bacteriol. (1989) [Pubmed]
  20. Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutations. Begg, K.J., Tomoyasu, T., Donachie, W.D., Khattar, M., Niki, H., Yamanaka, K., Hiraga, S., Ogura, T. J. Bacteriol. (1992) [Pubmed]
  21. Genetic analysis of the cell division protein FtsI (PBP3): amino acid substitutions that impair septal localization of FtsI and recruitment of FtsN. Wissel, M.C., Weiss, D.S. J. Bacteriol. (2004) [Pubmed]
  22. Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments. Taschner, P.E., Huls, P.G., Pas, E., Woldringh, C.L. J. Bacteriol. (1988) [Pubmed]
  23. Characterization of a new solvent-responsive gene locus in Pseudomonas putida F1 and its functionalization as a versatile biosensor. Phoenix, P., Keane, A., Patel, A., Bergeron, H., Ghoshal, S., Lau, P.C. Environ. Microbiol. (2003) [Pubmed]
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