Disease relevance of flgE

• The hook protein-encoding gene flgE of C. jejuni was cloned in Escherichia coli and sequenced [1].
• Beginning at 312 nt downstream from flgE was a partial ORF of 486 nt with a deduced amino acid sequence that was 33% identical to that of MotA of Bacillus subtilis, a polypeptide that enables flagellar rotation [2].
• The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease [3].
• Endometase mRNA was expressed specifically in human uterus, not in other tissues/cells tested, e.g. testis, heart, brain, lungs, liver, thymus, and melanoma G361 [4].
• Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12 [5].

High impact information on flgE

• Membranes of the flaK mutant were unable to process preflagellin in the in vitro assay [6].
• Homologous recombination of an integration vector into the chromosomal copy of flaK resulted in a non-motile, non-flagellated phenotype [6].
• The ORFs of flaH, flaI and flaK contain sequences coding for nucleotide binding sites [7].
• The genes flaD, E, G, H, I and J share high homologies with genes from other Archaea. Interestingly, flaK shows similarities to bacterial genes involved in the regulation of flagellar synthesis [7].
• OBJECTIVE: The factor V Leiden (FVL) mutation (Arg506Glu) results in the production of an FV protein that when activated is relatively resistant to inactivation by activated protein C and thereby leads to predisposition to thrombosis [8].

Chemical compound and disease context of flgE

• The renatured soluble form of FV/IL-2 was purified from E. coli inclusion bodies using hydroxylapatite chromatography [9].

Biological context of flgE

• Starting with a primer specific to flgE, we identified and sequenced 14 open reading frames (ORFs) spanning 11 kb downstream of the flgE gene [10].
• The T. phagedenis flgE gene was cloned into an Escherichia coli protein expression plasmid, and the purified recombinant protein was used to prepare a FlgE antiserum [2].
• Upstream of flgE, separated by 39 nt, was a partial (291-nt) ORF with a deduced amino acid sequence that was homologous to that of ORF8, a polypeptide of unknown function located in an operon encoding polypeptides involved in motility of B. subtilis [2].
• The flgE gene is 1,119 nucleotides long and is located on the 950-kb linear chromosome of B. burgdorferi [3].
• It was found that transcription of genes responsible for the formation and/or function of flagella (hag, fla, mot, che) decreased significantly at 40 degrees C. However, in the ftsI730 mutant at the nonpermissive temperature, or in penicillin G treated wild-type cells, cell division was blocked but formation of flagella continued [11].

Anatomical context of flgE

• No analogs to the bacterial chemotactic and motility (che, mot, fla) genes, genes for a two-component signal transduction system, genes associated with gliding, or genomic homologs for the eukaryotic cytoskeleton and motor proteins were found in the Mollicutes [12].

Analytical, diagnostic and therapeutic context of flgE

• The flgE genes of four additional C. jejuni strains were amplified by PCR and also sequenced [1].
• Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the genes flaE to flaK are transcribed into one polycistronic message [7].

Immunological context of flgE

• The flgE proteins, as antigens, could induce antibodies in hosts upon infection. [13-15]
• The flgE proteins, as immunostimulators, involve in lung injury in animal model. [16-17] 

References