Disease relevance of Eye Banks

• Total TGF-beta 2 levels in vitreous fluid were significantly lower in samples from patients with uveitis than in samples from eye bank eyes [1].
• Higher levels (P less than 0.05) of nucleoside monophosphates and choline phosphate and lower levels (P less than 0.05) of adenosine diphosphate were detected in keratoconus than in eye-bank corneas [2].
• The level of GSSG in the nucleus of human cataracts was significantly higher than that in the nucleus of normal eye bank lenses [3].

High impact information on Eye Banks

• CONCLUSIONS: Retinal tissue RNA can be analyzed within the time constraints of current eye bank tissue processing, whereas analysis of RPE necessitates either rapid processing or use of RNAlater [4].
• METHODS: Proteins from microdissected eye bank ocular ciliary body tissue were used to identify the corresponding ANCA antigen [5].
• METHODS: Eight-millimeter-diameter macular discs from 88 unpaired human eye bank eyes were obtained within 72 hours of death, fixed in 10% neutral buffered formalin, and hemisected horizontally [6].
• METHODS: The VISX Star (Santa Clara, CA) 193-nm argon fluoride excimer laser was used to ablate the cornea in human eye bank eyes under clinical conditions [7].
• In 8 of 10 samples taken from patients during cataract surgery and in 7 of 10 eye bank eyes the amount of IL-6 was significantly lower (mean, 10 pg/ml and 136 pg/ml, respectively) [8].

Anatomical context of Eye Banks

• METHODS: Monodispersed cell suspensions of human choroid were prepared from eye bank globes and the number, histamine content, and secretory response of mast cells in these preparations were determined [9].
• Human choroidal melanocytes were isolated from eye bank tissue and cultured in vitro with 10 ng/ml cholera toxin and 10 ng/ml phorbol myristate acetate [10].
• PURPOSE: Methyl methacrylate vascular corrosion casting techniques were used to examine the normal anterior optic nerve microvasculature in 18 human eye bank eyes [11].
• Glutathione reductase (GR) activity was measured with and without the addition of flavin adenine dinucleotide (FAD) in freshly excised human lens epithelium following cataract surgery, and in control eye bank lens epithelium [12].

Associations of Eye Banks with chemical compounds

• METHODS: Scleral tissue from 97 human eye bank eyes was tested individually in a two-chamber Ussing apparatus with the following hydrophilic radiolabeled compounds on one side of the chamber: 5-fluorouracil, sucrose, dexamethasone, methotrexate, inulin, and three separate dextran polymers (MWt = 10,000, 40,000, and 70,000) [13].
• Corneal penetration of sulfonamide carbonic anhydrase inhibitors for topical treatment of glaucoma has been tested in human eye bank and rabbit tissue [14].
• The concentration of P-choline in 53 clear human lenses was 0.94 mM (+/- 0.31 S.D.) and was relatively unaffected by age, eye bank storage, or freezing [15].
• Air bubble endothelial damage during phacoemulsification in human eye bank eyes: the protective effects of Healon and Viscoat [16].
• METHODS: The Sovereign phacoemulsification unit (Allergan) was used in 3 modes in conjunction with the Olson irrigating chopper (ASICO) in 6 fresh human cadaver eye-bank eyes to determine the temperature at which a wound burn would occur [17].

Gene context of Eye Banks

• Lenses from 42 eye bank eyes were assayed for glutathione peroxidase and glutathione reductase activities [18].
• METHODS: Twenty-two human whole globes not suitable for transplantation were obtained from an eye bank (San Diego Eye Bank, San Diego, California) and used for study [19].
• As EGF overcomes H2O2-induced rises in [Ca2+]i, its presence during eye bank storage could improve the outcome of corneal transplant surgery [20].
• OBJECTIVE: To evaluate the effects of the intrastromal corneal ring, a device developed to reduce myopia, on corneal asphericity in a large set of eye bank eyes [21].
• After correction for calibration error, the CLC readings on eye bank eyes were 1.00 D higher in average (range +0.45 to +1.90) than those obtained with the SK-1 which gave lower keratometric values at the 2-mm chord diameter (-0.254 D, range: +0.05 to -0.7) [22].

Analytical, diagnostic and therapeutic context of Eye Banks

• METHODS: Six human eye bank corneas, from individuals aged between 42 and 82 years, were received in corneal storage medium (Optisol GS) and then processed for transmission electron microscopy with buffered 2% glutaraldehyde fixation [23].

References