Disease relevance of Bronchoalveolar Lavage Fluid

• The effect of hyperoxia was assessed by parameters of O2-induced lung injury (e.g., hematocrit value, pleural effusion volume, effusion protein to plasma protein ratio, and alveolar lavage fluid protein content), TNF release into the alveolar space, and survival [1].
• Oleic acid also induced arterial hypoxemia, and increased bronchoalveolar lavage-fluid levels of immunoreactive (i) leukotriene LTC4 from 0.40 +/- 0.14 ng/ml to 2.27 +/- 0.55 ng/ml (mean +/- S.E.M., n = 4, P less than 0.05) and iLTB4 (from 0.42 +/- 0.05 ng/ml to 1.91 +/- 0.63 ng/ml, n = 5-7, P less than 0.01) [2].
• We report that alveolar lavage fluid obtained following acute lung injury induced by bleomycin in the rat contains large amounts of soluble growth factor activity not found in lung lavage fluid from normal animals [3].

High impact information on Bronchoalveolar Lavage Fluid

• The elevations were most remarkable in s-BALF and in lung tissue: We found a 170-fold increase in ADA activity and a 400-fold increase in XO activity as measured per volume of alveolar lavage fluid [4].
• Alpha-1-antitrypsin content in the serum, alveolar macrophages, and alveolar lavage fluid of smoking and nonsmoking normal subjects [5].
• Significant transudation of serum into alveolar lavage fluid occurred in bleomycin-treated rats [6].
• However, angiotensin-converting enzyme activity in alveolar lavage fluid from bleomycin-treated rats was elevated 30-fold above the barely detectable levels found in control animals [6].
• Bound sialic acid concentrations in bronchial alveolar lavage fluids, an index of mucus secretion, increased with DEP, but were suppressed by pretreatment with PEG-SOD [7].

Biological context of Bronchoalveolar Lavage Fluid

• The concentrations of IL4, IFNgamma, and soluble ICAM-1 in the bronchial alveolar lavage fluid increased twofold compared with values before rDer f 2 inhalation [8].
• Bronchoalveolar alveolar lavage fluid (BAL) of the left lung was analyzed for white cell count, differential, absolute number of PMNs, total protein, and cyclooxygenase products 6-keto-PGF1 alpha, and thromboxane B2 [9].

Anatomical context of Bronchoalveolar Lavage Fluid

• The cell-free fraction of alveolar lavage fluids from RBC- or RBC lysate-insufflated rats had similar capacity in protecting endothelial cells against H2O2 cytotoxicity [10].

Associations of Bronchoalveolar Lavage Fluid with chemical compounds

• Exposure to hyperoxia progressively increased the amounts of the surfactant proteins in alveolar lavage fluid as estimated by immunoblot analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis [11].
• The recovery of albumin and urea in alveolar lavage fluid was very similar in the two groups [12].
• DP-1904 (10 mg/kg) inhibited the increase in thromboxane level in both plasma and bronchial alveolar lavage fluid [13].
• The bradykinin concentrations in the bronchoalveolar lavage-fluid (BAL-f) were increased during the LAR [14].
• After this, alveolar lavage fluid was obtained for phosphatidylcholine content determination [15].

Gene context of Bronchoalveolar Lavage Fluid

• For asthmatic subjects in the Balmes study, IL-6 and IL-8 increased in both bronchial and alveolar lavage fluid, but not in proximal airway lavage fluid [16].
• No detectable amounts of TNF-alpha, IL-1-beta, and IL-6 were in bronchial alveolar lavage fluid, even after a tenfold concentration [17].
• Surfactant protein B (SP-B) decreased in alveolar type II cells at 5 h, and SP-B in lung tissue and alveolar lavage fluid increased by 72 h [18].
• These forms of SP-B (Mr = 8,000 and 12,000) comigrated in two-dimensional isoelectric-focusing sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mature SP-B isolated from rat alveolar lavage fluid [19].
• M8 antibody also did not decrease neutrophil counts or MPO in alveolar lavage fluid, both of which were significantly elevated in all oxygen-exposed groups [20].

Analytical, diagnostic and therapeutic context of Bronchoalveolar Lavage Fluid

• With immunologic techniques and polyacrylamide disc-gel electrophoresis the excess protein in the alveolar lavage fluid was shown to be derived from the circulation [21].

References