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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Local Lymph Node Assay

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Disease relevance of Local Lymph Node Assay


High impact information on Local Lymph Node Assay


Chemical compound and disease context of Local Lymph Node Assay


Biological context of Local Lymph Node Assay


Anatomical context of Local Lymph Node Assay


Associations of Local Lymph Node Assay with chemical compounds


Gene context of Local Lymph Node Assay

  • We have previously shown that a modified LLNA can be used to identify respiratory allergens, on the basis of Con A induced IL-4 production [10].
  • Secondly, another assay, similar to the modified LLNA but differing in the duration and the number of allergen applications as well as in the ex vivo culture conditions, here denoted as 'longer' assay, has been reported to be able to identify contact allergens, on the basis of (spontaneous) IFN-gamma production [10].
  • The IL-2-based LLNA showed similar performances at both qualitative and quantitative levels compared to regular LLNA [17].
  • Although previously determined to induce immunologically equivalent responses in a local lymph node assay (LLNA), the initial dose chosen (2.5%) failed to induce Th2 cytokine mRNA expression [18].
  • Cytokine production induced by low-molecular-weight chemicals as a function of the stimulation index in a modified local lymph node assay: an approach to discriminate contact sensitizers from respiratory sensitizers [19].

Analytical, diagnostic and therapeutic context of Local Lymph Node Assay


  1. Differences of draining lymph node cell proliferation among mice, rats and guinea pigs following exposure to metal allergens. Ikarashi, Y., Ohno, K., Tsuchiya, T., Nakamura, A. Toxicology (1992) [Pubmed]
  2. Local lymph node activation and IgE responses in brown Norway and Wistar rats after dermal application of sensitizing and non-sensitizing chemicals. Arts, J.H., Dröge, S.C., Spanhaak, S., Bloksma, N., Penninks, A.H., Kuper, C.F. Toxicology (1997) [Pubmed]
  3. Contact allergens formed on air exposure of linalool. Identification and quantification of primary and secondary oxidation products and the effect on skin sensitization. Sköld, M., Börje, A., Harambasic, E., Karlberg, A.T. Chem. Res. Toxicol. (2004) [Pubmed]
  4. Skin sensitization to eugenol and isoeugenol in mice: possible metabolic pathways involving ortho-quinone and quinone methide intermediates. Bertrand, F., Basketter, D.A., Roberts, D.W., Lepoittevin, J.P. Chem. Res. Toxicol. (1997) [Pubmed]
  5. The cytokine-dependent MUTZ-3 cell line as an in vitro model for the screening of contact sensitizers. Azam, P., Peiffer, J.L., Chamousset, D., Tissier, M.H., Bonnet, P.A., Vian, L., Fabre, I., Ourlin, J.C. Toxicol. Appl. Pharmacol. (2006) [Pubmed]
  6. Validity of methods to predict the respiratory sensitizing potential of chemicals: A study with a piperidinyl chlorotriazine derivative that caused an outbreak of occupational asthma. Vanoirbeek, J.A., Mandervelt, C., Cunningham, A.R., Hoet, P.H., Xu, H., Vanhooren, H.M., Nemery, B. Toxicol. Sci. (2003) [Pubmed]
  7. Insights into the quantitative relationship between sensitization and challenge for allergic contact dermatitis reactions. Scott, A.E., Kashon, M.L., Yucesoy, B., Luster, M.I., Tinkle, S.S. Toxicol. Appl. Pharmacol. (2002) [Pubmed]
  8. Assessment of contact sensitivity of four thiourea rubber accelerators: comparison of two mouse lymph node assays with the guinea pig maximization test. Ikarashi, Y., Ohno, K., Momma, J., Tsuchiya, T., Nakamura, A. Food Chem. Toxicol. (1994) [Pubmed]
  9. Quantitative structure-activity relationships: sulfonate esters in the local lymph node assay. Roberts, D.W., Basketter, D.A. Contact Derm. (2000) [Pubmed]
  10. Impact of exposure duration by low molecular weight compounds on interferon-gamma and interleukin-4 mRNA expression and production in the draining lymph nodes of mice. Vandebriel, R.J., De Jong, W.H., Hendriks, J.J., Van Loveren, H. Toxicology (2003) [Pubmed]
  11. Sensitization potential of gold sodium thiosulfate in mice and guinea pigs. Ikarashi, Y., Kaniwa, M., Tsuchiya, T. Biomaterials (2002) [Pubmed]
  12. ICCVAM evaluation of the murine local lymph node assay. Conclusions and recommendations of an independent scientific peer review panel. Dean, J.H., Twerdok, L.E., Tice, R.R., Sailstad, D.M., Hattan, D.G., Stokes, W.S. Regulatory toxicology and pharmacology : RTP. (2001) [Pubmed]
  13. Dietary vitamin A enhances sensitivity of the local lymph node assay. Sailstad, D.M., Krishnan, S.D., Tepper, J.S., Doerfler, D.L., Selgrade, M.K. Toxicology (1995) [Pubmed]
  14. Assessment of preferential T-helper 1 or T-helper 2 induction by low molecular weight compounds using the local lymph node assay in conjunction with RT-PCR and ELISA for interferon-gamma and interleukin-4. Vandebriel, R.J., De Jong, W.H., Spiekstra, S.W., Van Dijk, M., Fluitman, A., Garssen, J., Van Loveren, H. Toxicol. Appl. Pharmacol. (2000) [Pubmed]
  15. Effect of topical cis-urocanic acid on local lymph node activation during contact sensitization in mouse, rat and guinea-pig. Lauerma, A.I., Homey, B., Vohr, H.W., Lee, C.H., Bloom, E., Maibach, H.I. Br. J. Dermatol. (1996) [Pubmed]
  16. The effect of a vitamin A acetate diet on ultraviolet radiation-induced immune suppression as measured by contact hypersensitivity in mice. Sailstad, D.M., Boykin, E.H., Slade, R., Doerfler, D.L., Selgrade, M.K. Photochem. Photobiol. (2000) [Pubmed]
  17. Qualitative and quantitative evaluation of a local lymph node assay based on ex vivo interleukin-2 production. Azam, P., Peiffer, J.L., Ourlin, J.C., Bonnet, P.A., Tissier, M.H., Vian, L., Fabre, I. Toxicology (2005) [Pubmed]
  18. Identifying airway sensitizers: cytokine mRNA profiles induced by various anhydrides. Plitnick, L.M., Loveless, S.E., Ladics, G.S., Holsapple, M.P., Smialowicz, R.J., Woolhiser, M.R., Anderson, P.K., Smith, C., Selgrade, M.J. Toxicology (2003) [Pubmed]
  19. Cytokine production induced by low-molecular-weight chemicals as a function of the stimulation index in a modified local lymph node assay: an approach to discriminate contact sensitizers from respiratory sensitizers. Van Och, F.M., Van Loveren, H., De Jong, W.H., Vandebriel, R.J. Toxicol. Appl. Pharmacol. (2002) [Pubmed]
  20. Use of the local lymph node assay in assessment of immune function. van den Berg, F.A., Baken, K.A., Vermeulen, J.P., Gremmer, E.R., van Steeg, H., van Loveren, H. Toxicology (2005) [Pubmed]
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