Disease relevance of Fertilization in Vitro

• In both experimental situations, antibodies against ZP3 were shown to exhibit contraceptive activity, leading respectively to the induction of long-term infertility in the primate model and to the complete inhibition of human fertilization in vitro [1].

High impact information on Fertilization in Vitro

• There was, however, a moderate inhibitory effect on fertilization in vitro, which may be attributable to a direct action of antiserum to LDH-X on sperm [2].
• Mouse gamete interactions during fertilization in vitro. Chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction [3].
• The beta-cyclodextrins were also able to capacitate mouse sperm in the absence of BSA, as measured by the ability of the zona pellucida to induce the acrosome reaction and by successful fertilization in vitro [4].
• Serum antizona antibodies, but not anti-oocyte antibodies, inhibited mouse fertilization in vitro [5].
• Ova reaching the two-cell stage 40 h after fertilization in vitro were associated with the same FF concentrations of FSH, hCG, and PRL as those that cleaved to the four-cell stage [6].

Biological context of Fertilization in Vitro

• In a second series of experiments, the effect of taurine on preimplantation embryonic development after fertilization in vitro was studied [7].
• In spermatozoa preincubated under conditions known to support capacitation and fertilization in vitro, treatment with progesterone caused a time-dependent stimulation of phosphorylation of at least eight proteins ranging in size from approximately 20-220 kDa [8].
• These results, obtained using chemically defined conditions, indicate that glucose is required for stimulating fertilization in vitro of bovine oocytes and that synergistic action of caffeine and heparin appears independently of the reversing activity of caffeine on the inhibition of heparin-induced sperm capacitation by glucose [9].

Anatomical context of Fertilization in Vitro

• Effects of caffeine and casein phosphopeptides on fertilization in vitro of pig oocytes matured in culture [10].
• RESULTS: An N-formyl-methionyl-leucyl-phenylalanine (FMLP) provocation test was used to demonstrate that the presence of leukocytes in 28.5% of the sperm preparations was associated with elevated levels of spontaneous reactive oxygen species production, impaired movement, and a reduced capacity for fertilization in vitro [11].
• FSH or LH alone stimulated oocyte maturation, resulting in a significant increase in the rate of development to blastocysts following fertilization in vitro and embryo culture [12].

Associations of Fertilization in Vitro with chemical compounds

• Oviduct-specific glycoprotein modulates sperm-zona binding and improves efficiency of porcine fertilization in vitro [13].
• Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM [14].
• Sperm morphology, however, proved to be a more significant predictor of fertilization in vitro compared to the Acridine Orange test [15].
• The Acridine Orange test: determining the relationship between sperm morphology and fertilization in vitro [15].
• A sequential analysis of the effect of progesterone on specific sperm functions crucial to fertilization in vitro in infertile patients [16].

Gene context of Fertilization in Vitro

• Monoclonal antibody to human fertilization antigen-1 (FA-1) inhibits bovine fertilization in vitro: application in immunocontraception [17].
• Evidence of proacrosin molecule abnormality as a possible cause of low acrosin activity and unexplained failure of fertilization in vitro [18].
• Blocking of human fertilization in vitro by sera with sperm-immobilizing antibodies [19].
• Inhibition of bovine fertilization in vitro by antibodies to SP-10 [20].
• Furthermore, because the binding assay is highly correlated with fertilization capacity, these alterations in motility, as evidenced by CASA, probably have a minor effect on fertilization in vitro [21].

References