Disease relevance of N-hydroxycarbamic acid ethyl ester

• The mutagenic activity of ethyl N-hydroxycarbamate and its related compounds in Salmonella typhimurium [1].

High impact information on N-hydroxycarbamic acid ethyl ester

• To learn the effects of tumor inhibitors on chemically induced malformations, caffeine, antipain, and 13-trans-retinoic acid were given to pregnant ICR/Jcl mice after a single dose of urethan, N-hydroxyurethan, N-methyl-N-nitrosourea, N-ethyl-N-nitrosourea, or 4-nitroquinoline 1-oxide, which induces about 50% of the malformed fetuses [2].
• To clarify a mechanism independent of DNA adduct formation, we examined DNA damage induced by N-hydroxyurethane, a urethane metabolite, using 32P-5'-end-labeled DNA fragments [3].
• Electron spin resonance spectroscopy, utilizing Fe[N-(dithiocarboxy)sarcosine](3), demonstrated that nitric oxide (NO) was generated from hydroxylamine and esterase-treated N-hydroxyurethane [3].
• Although research on the metabolic activation of urethane has been extensive, no conclusive results have been obtained about its active metabolite, at one time thought to be N-hydroxyurethane [4].
• Administration of equimolar dosages (20 mmol/kg) of urethane, N-hydroxyurethane, and methyl carbamate indicated that urethane and N-hydroxyurethane induce MT but that methyl carbamate does not [5].

Biological context of N-hydroxycarbamic acid ethyl ester

• Ethyl carbamate (EC) and two related carcinogens, ethyl N-hydroxycarbamate (ENHC) and vinyl carbamate (VC), caused species-specific increases in sister-chromatid exchange (SCE) formation in the bone marrow cells of rodents [6].
• Acylation of ethyl N-hydroxycarbamate markedly enhanced its mutagenic activity for TA100 [1].
• Rodent species and strain specificities for sister-chromatid exchange induction and gene mutagenesis effects from ethyl carbamate, ethyl N-hydroxycarbamate, and vinyl carbamate [6].
• N-Hydroxyurea and two structurally related compounds, acetohydroxamic acid and N-hydroxyurethane, were investigated for their potential to induce DNA repair synthesis in primary rat hepatocyte cultures [7].

Anatomical context of N-hydroxycarbamic acid ethyl ester

• Urethane and hydroxyurethane induce sister-chromatid exchanges in cultured human lymphocytes [8].
• The present study demonstrated the metabolism of N-hydroxyurethane by cell-free preparations, i.e., 9000 X g supernatant, cytosol and microsomes, from guinea pig livers [9].
• When the 30-45% ammonium sulfate fraction from liver cytosol was combined with liver microsomes or milk xanthine oxidase, the metabolic reaction of N-hydroxyurethane proceeded to a greater extent [9].

Associations of N-hydroxycarbamic acid ethyl ester with other chemical compounds

• In addition, ethyl N-hydroxycarbamate, a P450 metabolite, suppressed the lymphoproliferative response induced by lipopolysaccharide and concanavalin A in splenocyte cultures [10].
• Guinea pig liver 9000 X g supernatant and cytosol, but not liver microsomes, exhibited N-hydroxyurethane reductase activity in the presence of acetaldehyde or 2-hydroxypyrimidine [11].

References