Disease relevance of EINECS 252-463-5

• Intense naphthol AS-D chloroacetate esterase (NASD) positivity was observed within most of the cells, suggesting granulocytic sarcoma [1].
• Two of 11 (18%) 2 degrees-ANLL patients had 10% or more naphthol-AS-D chloroacetate esterase-positive cells, in contrast to nine (47%) of 19 patients with ANLL de novo [2].

High impact information on EINECS 252-463-5

• MEG-01 cells were positive for the periodic acid-Schiff reaction, alpha-naphthyl acetate esterase, and acid phosphatase, and negative for myeloperoxidase, alpha-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase [3].
• The IMMCs in infected -/- mice stained poorly, if at all, for esterase with naphthol AS-D chloroacetate compared with the intense staining observed in +/+ controls [4].
• The presence of neutrophils, assessed by the measurement of liver tissue myeloperoxidase (MPO), and the degree of neutrophil liver infiltration, determined by the naphthol AS-D chloroacetate esterase technique, correlated well with animal survival and response to FK506 and cyclosporine administration [5].
• The results of the technique are compared with those described with staining for muramidase (lysozyme), alpha 1-antitrypsin, leukocyte elastase, and naphthol-AS-D-chloroacetate esterase, and with certain monoclonal antisera directed against granulocyte determinants [6].
• The non-specificity of the so-called neutrophil-specific identification methods (MPO activity, naphthol AS-D chloroacetate esterase, or mAb HIS-48 staining), cross-reacting with monocytes/macrophages, explains the controversy in literature concerning the number of PMNs in post-ischaemic injury [7].

Biological context of EINECS 252-463-5

• Investigation of granulocytopoietic kinetics by microdensitometric evaluation of primary granule naphthol-AS-D-chloroacetate esterase activity [8].
• The tissue distribution of PMNs in the kidneys was studied histochemically using naphthol AS-D chloroacetate esterase as a specific marker for these cells [9].
• Naphthol-AS-D-chloroacetate esterase activity can be observed in granules of the entire neutropil cell lineage [10].

Anatomical context of EINECS 252-463-5

• Unlike NAP reaction, naphthol-AS-D-chloroacetate esterase and peroxidase reactions are positive even in the earliest (AP negative) neutrophils [11].
• The established cell line was positive for myeloperoxidase, Sudan black B, Naphthol AS-D chloroacetate esterase [12].
• Of interest, two of these were devoid of naphthol AS-D chloroacetate esterase (CAE), which is prominently displayed in neutrophilic granulocytes, even though these leukemic cells were 100% intensely positive for MPO activity [13].
• Mast cells were detected in adjacent sections by the naphthol-AS-D-chloroacetate esterase technique [14].
• Staining with naphthol AS-D chloroacetate produced an intense red reaction product in intraepithelial mucosal mast cells (globule leucocytes) and mucosal mast cells within the lamina propria of the gastrointestinal tract [15].

Associations of EINECS 252-463-5 with other chemical compounds

• The enzyme activities of tryptase and chymase were tested using N-carbobenzoxy-glycilglycil-L-arginine-2-naphthylamide (Z-Gly-Gly-Arg-NA) and naphthol-AS-D-chloroacetate (N-AS-D-CA) as substrates, respectively [16].
• After nine days of in vitro culture, 50% of cells obtained from normal mice and 100% of cells obtained from schistosome-infected mice expressed chloroacetate esterases (naphthol AS-D chloroacetate esterases) [17].

Gene context of EINECS 252-463-5

• The cells were positively stained with alpha-naphtyl butyrate esterase, but not with naphthol AS-D chloroacetate esterase, myeloperoxidase, and periodic acid-Schiff reagent [18].
• The technique was carried out in subsequent steps demonstrating (a) naphthol AS-D chloroacetate esterase (CAE); (b) S100 immunoreactivity using a rabbit antibody in peroxidase-antiperoxidase (PAP) staining; and (c) Mac387 immunoreactivity using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) technique [19].
• Sections were stained with either naphthol AS-D chloroacetate esterase or KP1 (CD68) to identify granulocytic tumours [20].
• The typical and atypical mast cells were confirmed by Giemsa and Leder's naphthol-AS-D-chloroacetate esterase stains and by immunohistochemical staining for tryptase and KIT protein (CD117) [21].
• The glomerular endothelial IL-1 alpha expression coincided with a slight but significant increase in the number of the glomerular polymorphonuclear leukocytes as identified by naphthol AS-D chloroacetate esterase enzyme histochemical reaction [22].

Analytical, diagnostic and therapeutic context of EINECS 252-463-5

• Enzyme histochemical staining with naphthol AS-D chloroacetate localized to the same cells that exhibited specific immunofluorescence [23].

References