Disease relevance of tetramethylammonium

• Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis [1].

High impact information on tetramethylammonium

• Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries [2].
• This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition [2].
• The noncovalently bound FMN of the C30A mutant enzyme is also converted to 6-hydroxy-FMN and rendered inactive upon reduction with excess trimethylamine, but not by reduction with dithionite, even at high pH or in the presence of the effector tetramethylammonium chloride [3].
• Erythrocyte calcium content increases from 15-20 to 120-150 nmol/g hemoglobin after incubation for 2 h at 20 degree C in a 400 mosmol/kg, pH 7.8 solution containing 100 mM sodium chloride, 90 mM tetramethylammonium chloride, 1 mM potassium chloride, and 10 mM calcium chloride [4].
• The solvation of tetramethylammonium chloride (Me4NCl) and tetra-n-butylammonium chloride (Bu4NCl) in water-acetonitrile mixtures was investigated by mass spectrometry of clusters isolated from the solution [5].

Biological context of tetramethylammonium

• 1. The cholinomimetic substances acetylcholine, nicotine, tetramethylammonium chloride and carbachol were infused intracerebroventricularly (i.c.v.) into conscious, normotensive cats and their effects on behaviour, blood pressure and heart rate recorded [6].

Anatomical context of tetramethylammonium

• When culture medium NaC; is replaced with tetramethylammonium chloride, however, both normal and RSV-infected fibroblasts alike approach proliferative inactivity in the presence of 110 mM Na and both die off in the presence of 90 mM Na [7].

Associations of tetramethylammonium with other chemical compounds

• The hearts were perfused with a modified Krebs-Henseleit solution (KHS; control group) or KHS containing tetramethylammonium-chloride (TMA; 100 microM), which did not influence heart haemodynamics or extracellular potassium accumulation during global ischaemia (GI) [8].
• The mobile phase was 0.1 M triethylamine phosphate (pH 3.0) in water-tetrahydrofuran-methanol (69:1:30) containing 0.02 M tetramethylammonium chloride [9].
• In the presence of the inhibitor tetramethylammonium chloride a shift of +0.2 V was observed in the midpoint redox potential for conversion of the oxidized 6-S-cysteinyl-FMN to the flavin radical form [10].
• A mobile phase of 0.003 M tetramethylammonium chloride in water-methanol (3:2, pH 7.4) was used to separate phenobarbital from belladonna alkaloids on an octadecylsilane column in less than 7 min [11].
• Using short SSOPs (15 nucleotides each) in single temperature tetramethylammonium chloride (TMAC) hybridization and washing steps, 30 IEF-definable isotypes of HLA-A antigens could be unambiguously defined by their hybridization patterns [12].

Gene context of tetramethylammonium

• The results from experiments using an IVD-specific oligomer and stringent washes with 3.0 M tetramethylammonium chloride suggested that high GC content may have caused hybridization of the probe to the 3.6- and 4.8-kb species [13].
• 4. The influence of ionic strength on the conformation of histone H1 has been determined by measurement of the sedimentation coefficient in tetramethylammonium chloride solutions of up to 2.5 M and extrapolated to infinite ionic strength [14].
• A novel non-radioactive protocol for molecular generic HLA-DR typing is introduced, employing sequence specific oligonucleotides (SSOs) enzymatically 3'-labelled with biotin-14-dATP via terminal deoxynucleotidyltransferase in a tetramethylammonium chloride hybridization procedure [15].

Analytical, diagnostic and therapeutic context of tetramethylammonium

• Low concentrations of tetramethylammonium chloride increase yield and specificity of PCR [16].
• Differential scanning calorimetry, temperature-dependent NMR, and UV spectroscopy are used to investigate the helix-to-coil transition of poly(deoxyadenylylthymidine) [poly(dA-dT)] in 1 M NaCl and 1 M Me4NCl (tetramethylammonium chloride) [17].
• The effects of extracellular sodium or chloride were studied by replacement perfusion with tetramethylammonium-chloride (96 mmol/L) or sodium acetate (96 mmol/L) [18].
• A crude extract containing these enzymes was deposited onto the chromatography gel, washed, and eluted by a linear gradient of tetramethylammonium chloride (0-0.3 M) [19].
• This method involves sampling with a 37-mm PVC membrane filter at 1 to 2 L/min, recovery with 3 mL of 50:50 ethanol:water with 0.085% phosphoric acid and 0.04% tetramethylammonium chloride in an ultrasonic bath for 10 min, and analysis by high performance liquid chromatography with fluorescence detection [20].

References