Disease relevance of cis-4-hydroxyproline

• Sustained release of cis-hydroxyproline in the treatment of experimental proliferative vitreoretinopathy in rabbits [1].
• Results with 20 and 60 micrograms of cis-hydroxyproline showed a 48% decrease in the rate of traction retinal detachments [2].
• Cis-hydroxyproline-induced inhibition of pancreatic cancer cell growth is mediated by endoplasmic reticulum stress [3].
• We conclude that cis-hydroxyproline partially prevents the chemical, structural, and functional changes of bleomycin-induced pulmonary fibrosis in the hamster [4].

High impact information on cis-4-hydroxyproline

• Retention of nonhelical procollagen containing cis-hydroxyproline in rough endoplasmic reticulum [5].
• Cis-4-hydroxyproline (CHP), a relatively specific inhibitor of collagen secretion, was used to examine the role of extracellular collagen deposition in supporting hematopoiesis in long-term C57B1/6J mouse bone marrow cell cultures [6].
• The addition of cis-hydroxyproline to cultures established for 7 days also resulted in the inhibition of hepatocyte function [7].
• Since it was blocked in the presence of L-azetidine carboxylic acid or cis-hydroxyproline, inhibitors of collagen secretion, endothelial cell migration appeared to be dependent on the continual secretion of collagen [8].
• When cells were incubated with the proline analog cis-hydroxyproline, which inhibits correct triple helix formation and increases intracellular degradation, the amount of procollagen codistributing with the lysosome/endosome markers increased greatly [9].

Chemical compound and disease context of cis-4-hydroxyproline

• PURPOSE: To evaluate the efficacy of sustained-release cis-4-hydroxyproline (CHP), a proline analog that inhibits collagen secretion, on experimental proliferative vitreoretinopathy (PVR) in rabbits [1].

Biological context of cis-4-hydroxyproline

• Stimulated OPCs exposed to the PH inhibitor cis-hydroxyproline (cis-HP), compared to the noninhibiting isomer trans-HP, for 11 days showed a dose-dependent decrease in cell proliferation, the surviving cells showed increased alkaline phosphatase activity [10].

Anatomical context of cis-4-hydroxyproline

• cis-Hydroxyproline inhibits proliferation, collagen synthesis, attachment, and migration of cultured bovine retinal pigment epithelial cells [11].
• Acetylcholinesterase in cocultures of rat myotubes and spinal cord neurons: effects of collagenase and cis-hydroxyproline on molecular forms, intra- and extracellular distribution, and formation of patches at neuromuscular contacts [12].
• Electron microscopic studies revealed that cis-4-hydroxyproline treatment resulted in a derangement of the basement membrane in the ventral prostate [13].
• Inhibition of collagen synthesis with cis-hydroxyproline decreased the nerve-induced clustering of AChRs in muscle cells as well as that caused by exogenous agrin in muscle-only cultures [14].
• However, accumulation of p65 in nerve fibers at sites of muscle contact, a sign of presynaptic differentiation, was unaffected by cis-hydroxyproline treatment [14].

Associations of cis-4-hydroxyproline with other chemical compounds

• The isolated cells grew well in vitro and the growth was inhibited by D-valine or cis-hydroxyproline [15].
• Cis-hydroxyproline effects were reversible in that, following cessation of CHP treatment, the perturbed morphology, DNA content, and organ weight returned to the range of DHT-treated controls [16].
• METHODS: We examined the effect of two proline analogs, L-azetidine 2-carboxylic acid and cis-hydroxyproline, a prolyl hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate, and a lysyl hydroxylase inhibitor, minoxidil, on the spreading of epithelium of organ-cultured rabbit cornea [17].
• In conclusion, cis-hydroxyproline and also methylprednisolone-acetate may improve the function of regenerating peripheral nerve, while estrogen-progesterone does not seem to have this effect [18].

Gene context of cis-4-hydroxyproline

• Long-term cultures of high purity were obtained when the ECs were grown on mitomycin C-treated L929 feeder layers, in medium supplemented with cis-hydroxyproline and FGF-1 [19].
• In addition, even in cis-hydroxyproline-inhibited cultures, agrin was evident at more than 90% of contacts showing accumulation of p65 in the nerve terminal [14].
• Moreover, accumulation of HSPG at contacts was also inhibited in cis-hydroxyproline-treated cultures [14].
• Upon cis-hydroxyproline exposure, Dex medium has also distinguished NF1 cell ALP activity from that of other cell origins [20].
• Elastin accumulation, however, was not inhibited by cis-hydroxyproline [21].

Analytical, diagnostic and therapeutic context of cis-4-hydroxyproline

• Synthesis and characterization of magnetically responsive albumin microspheres containing cis-hydroxyproline for scar inhibition [22].

References