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Chemical Compound Review

fMet     2-formamido-4-methylsulfanyl- butanoic acid

Synonyms: for-met-oh, ACMC-1AHIA, AG-K-70481, ACMC-209jrn, F3377_SIGMA, ...
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Disease relevance of N-FORMYLMETHIONINE


High impact information on N-FORMYLMETHIONINE


Chemical compound and disease context of N-FORMYLMETHIONINE


Biological context of N-FORMYLMETHIONINE


Anatomical context of N-FORMYLMETHIONINE


Associations of N-FORMYLMETHIONINE with other chemical compounds

  • Polyethylene glycol is shown to be a competitive inhibitor with a KI value of 6 mM with respect to formylmethionine under conditions similar to those used for crystallization [25].
  • Unlike calcium ionophore, however, pretreatment of basophils with diatrizoate may also induce dose-dependent inhibition of reactivity during subsequent challenges with anti-IgE, N-formyl methionine peptide, and calcium ionophore [26].
  • Here we show that the reaction of P-site formylmethionine (fMet)-tRNA(fMet) with a modified A-site tRNA substrate, Phelac-tRNA(Phe), in which the nucleophilic amino group is replaced with a hydroxyl group, does not show the pH dependence observed with small substrate analogs such as puromycin and hydroxypuromycin [27].


  • VP1, VP2, and vp3 were all labeled with [35S] formylmethionine when they were synthesized in the presence of [35S] formylmethionyl-tRNAfmet [28].
  • Expression of Escherichia coli methionyl-tRNA formyltransferase in Saccharomyces cerevisiae leads to formylation of the cytoplasmic initiator tRNA and possibly to initiation of protein synthesis with formylmethionine [16].
  • Galectin expression was up-regulated in inflammatory and activated macrophages, revealing a significant increase in phorbol ester- and formylmethionine oligopeptide-treated cells [29].
  • The H2-M3 class Ib gene product, M3, preferentially presents formylmethionine-initiating (fMet) peptides derived from the N termini of bacterial and mitochondrial proteins [30].
  • In order to cleave RNA at specific positions in Escherichia coli formylmethionine tRNA, RNase H and complementary chimeric oligonucleotides consisting of DNA and 2'-O-methyl-RNA (Inoue et al [31].

Analytical, diagnostic and therapeutic context of N-FORMYLMETHIONINE

  • The molecular mass of 3,794 Da as determined by MALDI-MS and by ESI requires the N-terminal methionine to be formylated and is in good agreement with the value calculated from the formylmethionine containing sequence (3,766.5 Da + 28 Da = 3,794.5 Da) [32].


  1. H-2M3 presents a Listeria monocytogenes peptide to cytotoxic T lymphocytes. Pamer, E.G., Wang, C.R., Flaherty, L., Lindahl, K.F., Bevan, M.J. Cell (1992) [Pubmed]
  2. Total synthesis of a RNA molecule with sequence identical to that of Escherichia coli formylmethionine tRNA. Ohtsuka, E., Tanaka, S., Tanaka, T., Miyake, T., Markham, A.F., Nakagawa, E., Wakabayashi, T., Taniyama, Y., Nishikawa, S., Fukumoto, R., Uemura, H., Doi, T., Tokunaga, T., Ikehara, M. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  3. Methionine and formylmethionine specific tRNAs coded by bacteriophage T5. Chen, M.J., Shiau, R.P., Hwang, L.T., Vaughan, J., Weiss, S.B. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  4. Variable immunodominance hierarchies for H2-M3-restricted N-formyl peptides following bacterial infection. Kerksiek, K.M., Busch, D.H., Pamer, E.G. J. Immunol. (2001) [Pubmed]
  5. The amino terminus of the aspartate chemoreceptor is formylmethionine. Milligan, D.L., Koshland, D.E. J. Biol. Chem. (1990) [Pubmed]
  6. Maternally transmitted antigen of mice: a model transplantation antigen. Fischer Lindahl, K., Hermel, E., Loveland, B.E., Wang, C.R. Annu. Rev. Immunol. (1991) [Pubmed]
  7. Mutants of Escherichia coli formylmethionine tRNA: a single base change enables initiator tRNA to act as an elongator in vitro. Seong, B.L., RajBhandary, U.L. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  8. Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop. Seong, B.L., RajBhandary, U.L. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  9. Anticodon loop size and sequence requirements for recognition of formylmethionine tRNA by methionyl-tRNA synthetase. Schulman, L.H., Pelka, H. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  10. Reinitiation of translation from the triplet next to the amber termination codon in the absence of ribosome-releasing factor. Ryoji, M., Berland, R., Kaji, A. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  11. Effect of the amino acid attached to Escherichia coli initiator tRNA on its affinity for the initiation factor IF2 and on the IF2 dependence of its binding to the ribosome. Wu, X.Q., RajBhandary, U.L. J. Biol. Chem. (1997) [Pubmed]
  12. Polyuridylic acid-dependent binding of fMet-tRNA to Escherichia coli ribosomes and incorporation of formylmethionine into polyphenylalanine. Van Der Laken, K., Bakker-Steeneveld, H., Van Knippenberg, P. FEBS Lett. (1979) [Pubmed]
  13. Characterization of the fMet initiator tRNA gene of Bartonella bacilliformis. Minnick, M.F. Gene (1993) [Pubmed]
  14. Characterization of a high-throughput screening assay for inhibitors of elongation factor p and ribosomal peptidyl transferase activity. Swaney, S., McCroskey, M., Shinabarger, D., Wang, Z., Turner, B.A., Parker, C.N. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening. (2006) [Pubmed]
  15. Modification of specific lysine residues in E. coli methionyl-tRNA synthetase by crosslinking to E. coli formylmethionine tRNA. Valenzuela, D., Leon, O., Schulman, L.H. Biochem. Biophys. Res. Commun. (1984) [Pubmed]
  16. Expression of Escherichia coli methionyl-tRNA formyltransferase in Saccharomyces cerevisiae leads to formylation of the cytoplasmic initiator tRNA and possibly to initiation of protein synthesis with formylmethionine. Ramesh, V., Köhrer, C., RajBhandary, U.L. Mol. Cell. Biol. (2002) [Pubmed]
  17. Alteration of the kinetic parameters for aminoacylation of Escherichia coli formylmethionine transfer RNA by modification of an anticodon base. Schulman, L.H., Pelka, H. J. Biol. Chem. (1977) [Pubmed]
  18. Mapping and cloning of Neurospora crassa mitochondrial transfer RNA genes. Heckman, J.E., Yin, S., Alzner-DeWeerd, B., RajBhandary, U.L. J. Biol. Chem. (1979) [Pubmed]
  19. The nucleotide sequence of formylmethionine tRNA from Mycoplasma mycoides sp. capri. Walker, R.T., RajBhandary, U.L. Nucleic Acids Res. (1978) [Pubmed]
  20. Reversible inactivation of Escherichia coli methionyl-tRNA synthetase by covalent attachment of formylmethionine tRNA to the tRNA binding site with a cleavable cross-linker. Schulman, L.H., Valenzuela, D., Pelka, H. Biochemistry (1981) [Pubmed]
  21. N-formylmethionyl peptides as chemoattractants for leucocytes. Schiffmann, E., Corcoran, B.A., Wahl, S.M. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  22. Effect of base sequence on in vitro protein-chain termination. Ganoza, M.C., Buckingham, K., Hader, P., Neilson, T. J. Biol. Chem. (1984) [Pubmed]
  23. Promiscuity of MHC class Ib-restricted T cell responses. Ploss, A., Lauvau, G., Contos, B., Kerksiek, K.M., Guirnalda, P.D., Leiner, I., Lenz, L.L., Bevan, M.J., Pamer, E.G. J. Immunol. (2003) [Pubmed]
  24. Use of formylated yeast initiator Met tRNA to define the NH2-terminal residues of rat preproinsulin and pregrowth hormone. Chan, S.J., Ackerman, E.J., Quinn, P.S., Sigler, P.B., Steiner, D.F. J. Biol. Chem. (1981) [Pubmed]
  25. Structure of peptide deformylase and identification of the substrate binding site. Becker, A., Schlichting, I., Kabsch, W., Schultz, S., Wagner, A.F. J. Biol. Chem. (1998) [Pubmed]
  26. Characteristics of diatrizoate-induced basophil histamine release. Younger, R.E., Herrod, H.G., Lieberman, P.L., Trouy, R.L., Crawford, L.V. J. Allergy Clin. Immunol. (1986) [Pubmed]
  27. Peptide bond formation does not involve acid-base catalysis by ribosomal residues. Bieling, P., Beringer, M., Adio, S., Rodnina, M.V. Nat. Struct. Mol. Biol. (2006) [Pubmed]
  28. Characterization of the mRNA's for the polyoma virus capsid proteins VP1, VP2, and VP3. Hunter, T., Gibson, W. J. Virol. (1978) [Pubmed]
  29. Activated rat macrophages produce a galectin-1-like protein that induces apoptosis of T cells: biochemical and functional characterization. Rabinovich, G.A., Iglesias, M.M., Modesti, N.M., Castagna, L.F., Wolfenstein-Todel, C., Riera, C.M., Sotomayor, C.E. J. Immunol. (1998) [Pubmed]
  30. CTL responses to H2-M3-restricted Listeria epitopes. Lenz, L.L., Bevan, M.J. Immunol. Rev. (1997) [Pubmed]
  31. Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides. Hayase, Y., Inoue, H., Ohtsuka, E. Biochemistry (1990) [Pubmed]
  32. Primary structure of a novel subunit in ba3-cytochrome oxidase from Thermus thermophilus. Soulimane, T., Than, M.E., Dewor, M., Huber, R., Buse, G. Protein Sci. (2000) [Pubmed]
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