Disease relevance of Car8

• In confirmation and extension of observations by Carp and his associates, brain tissue and sera from patients with multiple sclerosis (MS) were found to harbor an agent which induces a transitory depression in polymorphonuclear leukocytes (PMN) in mice as well as in rats, hamsters, and guinea pigs [1].
• The present findings suggest that CA-RP VIII may play a role in non-small lung cell carcinomas [2].
• In the present study, CA-RP VIII expression in non-tumorous lung and non-small cell lung carcinomas was investigated [2].
• Neither bronchial gland nor squamous metaplasia of interstitial pneumonia expressed CA-RP VIII [2].
• In contrast, CA-RP VIII was strongly expressed in almost all archival lung cancer specimens, which included 24 squamous cell carcinomas, 25 adenocarcinomas, and 6 adenosquamous cell carcinomas [2].

High impact information on Car8

• A 19-bp deletion in exon 8 of the carbonic anhydrase-related protein VIII gene (Car8) was detected by high-throughput temperature-gradient capillary electrophoresis heteroduplex analysis of PCR amplicons of genes and ESTs within the wdl locus on mouse chromosome 4 [3].
• The expression of CA IV, CA VII and CA-RP VIII was somewhat similar: they were expressed in the cortex, hippocampus and midbrain structures and their expression did not change after the kainic acid treatment [4].
• 3. Despite potentially inactivating changes in the active-site residues, CA-RP XI is evolving very slowly in mammals, a property indicative of an important function, which has also been observed in the two other "acatalytic" CA isoforms, CA-RP VIII and CA-RP X, whose functions are unknown [5].
• Sequence of a novel carbonic anhydrase-related polypeptide and its exclusive presence in Purkinje cells [6].
• In murine embryos, CA-RP VIII and X messages first appeared in the middle of the gestation period, while the CA-RP XI message was seen at an early gestational stage and expressed to a lesser extent as gestation progressed [7].

Biological context of Car8

• Reverse-transcription polymerase chain reaction analysis demonstrated CA-RP VIII mRNA in developing human lungs and, to a lesser extent, in normal lungs [2].

Anatomical context of Car8

• In the cerebellum, both Car7 and Car8 were transcribed to different degrees in the Purkinje cells, and a lower expression level occurred in the molecular and granular cell layers [8].
• The level of expression of Car8 mRNA was relatively high in the brain, liver, lung, heart, gut, thymus and epithelium covering the head and the oronasal cavity [9].
• Results indicate that this gene (called Car8 in mice) is expressed as early as day 9.5 in a variety of organs including liver, branchial arches, neuroepithelium and developing myocardium [9].
• Carbonic anhydrase II and carbonic anhydrase-related protein in the cerebellar cortex of normal and lurcher mice [10].

Analytical, diagnostic and therapeutic context of Car8

• Little or no expression of CA-RP VIII was observed in human lungs by Northern-blot analysis [2].

References