Disease relevance of ADRA2B

• The alpha 2B adrenergic receptor of undifferentiated neuroblastoma x glioma hybrid NG108-15 cells, interacts directly with the guanine nucleotide binding protein, Gi2 [1].

High impact information on ADRA2B

• We have isolated a cDNA clone (RB alpha 2B) and its homologous gene (GR alpha 2B) encoding an alpha 2B-adrenergic receptor subtype by screening a rat brain cDNA and a rat genomic library [2].
• We provide a simultaneous reconstruction of phylogeny and age of the four radiations based on a 3.5-kb data set from three nuclear genes (ADRA2B, vWF, and AR) [3].
• Polymorphic deletion of three intracellular acidic residues of the alpha 2B-adrenergic receptor decreases G protein-coupled receptor kinase-mediated phosphorylation and desensitization [4].
• In this study we investigated whether the 12Glu9 polymorphism in the gene encoding the alpha2B-adrenergic receptor ( ADRA2B) is associated with insulin secretion and/or the incidence of Type 2 diabetes in individuals with impaired glucose tolerance [5].
• Several common polymorphisms (>10% minor allele frequency) at ADRA2B (insertion/deletion polymorphism [Glu 322-325 ], G-98C, C1182A, and C1776A) did not show an association with either B max or K d for the drug response [6].

Biological context of ADRA2B

• Cardiovascular and metabolic phenotypes in relation to the ADRA2B insertion/deletion polymorphism in a Chinese population [7].
• CONCLUSION: In Chinese men, the I allele of the ADRA2B gene is associated with higher blood pressure, but also with a more favourable metabolic phenotype [7].
• Subtype-specific probes to the murine homologs of the human ADRA2B and ADRA2C genes were prepared by PCR amplification and used to map these two genes to mouse chromosomes 2 and 5, respectively [8].
• ADRA2A and ADRA2B each had a single haplotype block at least 11 and 16 kb in size, respectively, in both populations [9].
• In contrast, inhibition curves for both ligands in the human caudate nucleus were consistent with a model of two classes of binding sites in approximately equal proportions, suggesting that this tissue contains approximately equal densities of the alpha-2A and alpha-2B adrenergic receptor subtypes [10].

Anatomical context of ADRA2B

• All Purkinje cells label strongly for alpha 2a and alpha 2b adrenergic receptor mRNA [11].
• Cervical spinal cord demonstrates a predominance of alpha 2a mRNA signal, while thoracic, lumbar, and sacral spinal cord demonstrate an increasing predominance of alpha 2bAR mRNA [12].
• The modulation of [3H]noradrenaline and [3H]serotonin release from rat brain synaptosomes is not mediated by the alpha 2B-adrenoceptor subtype [13].
• Competition studies with alpha 2-adrenoceptor ligands characterized the presence of the alpha 2A-adrenoceptor subtype in adipocytes, colocytes, kidney and brain homogenates and of the alpha 2B-adrenoceptor subtype in kidney and liver [14].
• We used LLC-PK1 cells stably transfected with cDNA encoding the alpha 2B-adrenergic receptor (alpha 2B-AR) or A1-adenosine receptor (A1-AdR) [15].

Associations of ADRA2B with chemical compounds

• These responses to azepexole show prominent differences as a function of age and gender but appear not to depend on common allelic variations at the ADRA2B receptor [6].
• Therefore whether the ADRA2B I/D polymorphism is associated with C1 and C2 need to be investigated [16].
• The present study aimed at relating the presynaptic alpha 2-adrenoceptors, known to modulate noradrenaline and serotonin release, with the recently described alpha 2A- and alpha 2B-adrenoceptor subtypes [13].
• 5. Classification is done mainly with alpha 2A-adrenoceptor-selective oxymetazoline, WB 4101 and BRL 44408, and the alpha 2B-adrenoceptor-selective prazosin, AR-C 239, chlorpromazine and BRL 41992 [17].
• To examine the alpha 2A/alpha 2B-adrenoceptor ratio in the different brain regions, we performed oxymetazoline and prazosin/[3H]RX 821002 competition binding experiments [18].

Other interactions of ADRA2B

• ADRA2B genotypes were determined by polymerase chain reaction [16].
• The alpha 2B adrenergic receptor (A2AB) is a heptahelical G protein-coupled receptor for catecholamines [19].
• RENAL ADRENOCEPTOR FUNCTION: Renal alpha 1- and alpha 2B-adrenoceptor numbers are frequently increased in genetically hypertensive rats but alpha 1-adrenoceptor-stimulated inositol phosphate formation is unchanged or decreased and alpha 2-adrenoceptor functions remain unclear [20].

Analytical, diagnostic and therapeutic context of ADRA2B

• CONCLUSION: The study do not support that the ADRA2B gene I/D polymorphism has a major gene effect on C1 or C2 in the Chinese population of current sample size [16].
• Northern blot analysis was performed using poly(A)+ RNA isolated from 90% confluent rat IMCD cells and probes derived from alpha 2-adrenoceptor DNA sequences from the rat nonglycosylated alpha 2B-adrenoceptor and the human alpha 2A-adrenoceptor [21].

References