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Gene Review

Ncl  -  nucleolin

Mus musculus

Synonyms: B530004O11Rik, C23, D0Nds28, D1Nds28, Nuc, ...
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Disease relevance of Ncl


High impact information on Ncl


Chemical compound and disease context of Ncl

  • The human monoclonal antibodies produced by them, C1 and C23, bound to CMV but not to other herpesviruses [11].
  • The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ss-lactoglobulin (BLG), in Lactococcus lactis were investigated [12].

Biological context of Ncl

  • Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons [13].
  • We describe the molecular organization and complete sequence of the mouse nucleolin gene, the first higher eukaryotic gene encoding a protein that is both an RNA binding protein involved in rRNA processing and a specific nucleolar protein [13].
  • The nucleolin gene extends over 9000 base-pairs and is split into 14 exons that encode the 706 amino acid residues of the protein [13].
  • Nucleolin phosphorylation was reduced to 20% of control [1].
  • Thus, in addition to the many known functions of nucleolin, such as rRNA transcription, processing, ribosome biogenesis, and the shuttling of proteins between the cytoplasmic and nuclear compartments, it may also function as a transcriptional repressor [2].

Anatomical context of Ncl


Associations of Ncl with chemical compounds

  • Nucleolin (713 aa), a major nucleolar protein, presents two structural domains: a N-terminus implicated in interaction with chromatin and a C-terminus containing four RNA-binding domains (RRMs) and a glycine/arginine-rich domain mainly involved in pre-rRNA packaging [18].
  • NDH II migrated out of the nucleolus after administration of 0.05 microg/ml actinomycin D, while nucleolin and the upstream binding factor (UBF) remained there [19].
  • Nucleolin protein was reduced to 40% of control in nuclei from dexamethasone-treated cells [1].
  • Cycloheximide (10 micrograms/ml for 3 h) reduced the amount and activity of casein kinase II, but did not cause a decrease in nucleolin protein [1].
  • The murine nucleolin protein is an inducible DNA and ATP binding protein which is readily detected in nuclear extracts of lipopolysaccharide-treated splenocytes [20].

Other interactions of Ncl


Analytical, diagnostic and therapeutic context of Ncl


  1. Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells. Suzuki, N., Suzuki, T., Uchida, A., Thompson, E.A., Hosoya, T. J. Steroid Biochem. Mol. Biol. (1992) [Pubmed]
  2. Purification and characterization of nucleolin and its identification as a transcription repressor. Yang, T.H., Tsai, W.H., Lee, Y.M., Lei, H.Y., Lai, M.Y., Chen, D.S., Yeh, N.H., Lee, S.C. Mol. Cell. Biol. (1994) [Pubmed]
  3. Interaction of the coronavirus nucleoprotein with nucleolar antigens and the host cell. Chen, H., Wurm, T., Britton, P., Brooks, G., Hiscox, J.A. J. Virol. (2002) [Pubmed]
  4. A 110-kDa nuclear shuttle protein, nucleolin, specifically binds to adeno-associated virus type 2 (AAV-2) capsid. Qiu, J., Brown, K.E. Virology (1999) [Pubmed]
  5. Phenobarbitone-induced liver response in wild type and in p53 deficient mice. Gould, S., Sidaway, J., Sansom, N., Betton, G., Orton, T. Toxicol. Lett. (2001) [Pubmed]
  6. Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Takagi, M., Absalon, M.J., McLure, K.G., Kastan, M.B. Cell (2005) [Pubmed]
  7. Nucleolin functions in the first step of ribosomal RNA processing. Ginisty, H., Amalric, F., Bouvet, P. EMBO J. (1998) [Pubmed]
  8. A possible mechanism for the inhibition of ribosomal RNA gene transcription during mitosis. Weisenberger, D., Scheer, U. J. Cell Biol. (1995) [Pubmed]
  9. The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein kinase II and nucleolin. Jin, Y.J., Burakoff, S.J. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  10. Nuclear targeting by the growth factor midkine. Shibata, Y., Muramatsu, T., Hirai, M., Inui, T., Kimura, T., Saito, H., McCormick, L.M., Bu, G., Kadomatsu, K. Mol. Cell. Biol. (2002) [Pubmed]
  11. Generation of hybridomas producing human monoclonal antibodies against human cytomegalovirus. Matsumoto, Y., Sugano, T., Miyamoto, C., Masuho, Y. Biochem. Biophys. Res. Commun. (1986) [Pubmed]
  12. Improvement of bovine ss-lactoglobulin production and secretion by Lactococcus lactis. Nouaille, S., Bermúdez-Humarán, L.G., Adel-Patient, K., Commissaire, J., Gruss, A., Wal, J.M., Azevedo, V., Langella, P., Chatel, J.M. Braz. J. Med. Biol. Res. (2005) [Pubmed]
  13. Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons. Bourbon, H.M., Lapeyre, B., Amalric, F. J. Mol. Biol. (1988) [Pubmed]
  14. Nucleolar distribution of proteins B23 and nucleolin in mouse preimplantation embryos as visualized by immunoelectron microscopy. Biggiogera, M., Bürki, K., Kaufmann, S.H., Shaper, J.H., Gas, N., Amalric, F., Fakan, S. Development (1990) [Pubmed]
  15. Increased stability of nucleophosmin/B23 in anti-apoptotic effect of ras during serum deprivation. Chou, C.C., Yung, B.Y. Mol. Pharmacol. (2001) [Pubmed]
  16. Identification and partial characterization of a Mr 40,000 nucleolar antigen associated with cell proliferation. Chatterjee, A., Freeman, J.W., Busch, H. Cancer Res. (1987) [Pubmed]
  17. Insulin induces the phosphorylation of nucleolin. A possible mechanism of insulin-induced RNA efflux from nuclei. Csermely, P., Schnaider, T., Cheatham, B., Olson, M.O., Kahn, C.R. J. Biol. Chem. (1993) [Pubmed]
  18. Determination of the functional domains involved in nucleolar targeting of nucleolin. Créancier, L., Prats, H., Zanibellato, C., Amalric, F., Bugler, B. Mol. Biol. Cell (1993) [Pubmed]
  19. Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolus. Zhang, S., Köhler, C., Hemmerich, P., Grosse, F. Exp. Cell Res. (2004) [Pubmed]
  20. The murine nucleolin protein is an inducible DNA and ATP binding protein which is readily detected in nuclear extracts of lipopolysaccharide-treated splenocytes. Miranda, G.A., Chokler, I., Aguilera, R.J. Exp. Cell Res. (1995) [Pubmed]
  21. Nucleolin as the earliest target molecule of autoantibodies produced in MRL/lpr lupus-prone mice. Hirata, D., Iwamoto, M., Yoshio, T., Okazaki, H., Masuyama, J., Mimori, A., Minota, S. Clin. Immunol. (2000) [Pubmed]
  22. U82, a novel snoRNA identified from the fifth intron of human and mouse nucleolin gene. Rebane, A., Metspalu, A. Biochim. Biophys. Acta (1999) [Pubmed]
  23. Localization to the nucleolus is a common feature of coronavirus nucleoproteins, and the protein may disrupt host cell division. Wurm, T., Chen, H., Hodgson, T., Britton, P., Brooks, G., Hiscox, J.A. J. Virol. (2001) [Pubmed]
  24. Development-dependent localization of nuclear antigens in growing mouse oocytes. Borsuk, E., Vautier, D., Szöllösi, M.S., Besombes, D., Debey, P. Mol. Reprod. Dev. (1996) [Pubmed]
  25. Epitope distribution and immunochemical characterization of nucleolar phosphoprotein C23 using ten monoclonal antibodies. Freeman, J.W., Chatterjee, A., Ross, B.E., Busch, H. Mol. Cell. Biochem. (1985) [Pubmed]
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