Disease relevance of bi

• Rare phenotypical features among mutation positive patients include hypothyroidism, vaginal aplasia with bifid uterus, cryptorchidism, bifid scrotum without hypospadia scrotalis, unilateral chorioretinal coloboma with loss of vision, dorsal hypoplasia of the corpus callosum, and umbilical hernia [1].

High impact information on bi

• Mutations in the high-affinity Brk binding site abolish responsiveness of this omb enhancer to Brk and also compromise the input of an unknown transcriptional activator [2].
• Here we report the molecular dissection of the cis-regulatory sequences of optomotor-blind (omb), a Dpp target gene in the wing [2].
• Using this omb wing enhancer, we show that Brk is a sequence-specific DNA binding protein [2].
• Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila [3].
• The lacZ reporter gene within P[lacW] exhibits the same expression pattern as omb [3].

Biological context of bi

• This induction of the L5 primordium is mediated by abrupt, which is expressed in a narrow stripe of cells along the brk/omb border and plays a key role in organizing gene expression in the L5 primordium [4].
• The wings of optomotor-blind mutants have two apparently opposite phenotypes: the central wing is severely reduced and shows massive cell death, mainly in the distal-most wing, and the lateral wing shows extra cell proliferation [5].
• In cell culture transfection assays, ORG-1 was a strong transcriptional activator, whereas OMB appeared neutral [6].
• The functional properties of OMB/ORG-1 chimeras in several developmental contexts was dominated by the origin of the C-terminal region, suggesting that the transcriptional activation potential can be one major determinant of developmental specificity [6].
• Transcript identification in the optomotor-blind locus of Drosophila melanogaster by intragenic recombination mapping and PCR-aided sequence analysis of lethal point mutations [7].

Anatomical context of bi

• The lethal(1)optomotor-blind gene of Drosophila melanogaster is a major organizer of optic lobe development: isolation and characterization of the gene [8].
• Cells in the ventral and peripheral nervous systems begin to express omb after completion of germ band extension [9].
• Expression of the Drosophila optomotor-blind gene transcript in neuronal and glial cells of the developing nervous system [9].
• In the first, terminal bifid type, a lateral branch arises and immediately bifurcates to form two terminal branches whose tips induce the formation of nephrons [10].
• The omb gene of the millipede Glomeris marginata is expressed on the dorsal side of all appendages including trunk legs, maxillae, mandibles, and antennae [11].

Associations of bi with chemical compounds

• Amperometric measurements were performed at 100 mV and -150 mV relative to the Ag/AgCl reference electrode for the mono-enzyme and bi-enzyme systems [12].

Regulatory relationships of bi

• Mosaic analysis indicates that brk-expressing cells produce a short-range signal that can induce vein formation in adjacent omb-expressing cells [4].
• We present evidence that Hedgehog induces X production by driving optomotor blind expression [13].

Other interactions of bi

• The lack of optomotor-blind produces massive cell death in its expression domain, which leads to the mis-activation of the Notch pathway and the overproliferation of lateral wing cells [5].
• Comparison of the expression profiles of H15 and optomotor-blind to the Drosophila patterns suggests modifications also in the dorsal-ventral patterning system of the legs [14].
• At the core of the locus lies a lethal complementation group, named l(1)omb, whose mutations cause larval and pupal lethality [15].

Analytical, diagnostic and therapeutic context of bi

• Amplification of mutant DNA by the polymerase chain reaction (PCR) and sequencing of the amplified exons revealed the presence of mutations that lead to truncation of the T3 open reading frame [7].
• We describe here the expression of omb in the developing and adult nervous system using in situ hybridization [9].
• Facilitated isolation of rare recombinants by ligase chain reaction: selection for intragenic crossover events in the Drosophila optomotor-blind gene [16].

References