Psychiatry related information on IGFBP2

• In kidney, IGFBP-2 mRNA was detected but not influenced by food deprivation and refeeding [1].

High impact information on IGFBP2

• To determine the developmental expression and localization of mRNA for insulin-like growth factor binding protein-2 (IGFBP-2), a major binding protein of IGF-I and IGF-II, in ocular tissues of the embryonic and early posthatched chick [2].
• Northern blot analysis confirms that a high level of IGFBP-2 expression is present in the cornea and sclera by E8 to E12 [2].
• In the eye, IGFBP-2 mRNA is expressed only in the presumptive corneal epithelium at this time [2].
• Wholemount in situ hybridization reveals that, as early as 3.5 days of embryonic development (E3.5), IGFBP-2 mRNA is already expressed in many areas of the embryo, including surface ectoderm, certain regions of the brain, pharyngeal clefts, somites, and limb buds [2].
• By E6, IGFBP-2 mRNA expression is present in both the corneal epithelium and endothelium [2].

Biological context of IGFBP2

• This research suggests that IGFBP2 or a tightly linked gene has broad effects on growth and development in the chicken [3].
• The current study was designed to investigate the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits [3].
• Insulin-like growth factor (IGF) -1 and -2 and binding protein (IGFBP-2, -4, and -5) expression can be affected by several environmental factors, but the impact of movement on the IGF axis during late embryogenesis has yet to be fully characterized [4].
• The chicken IGFBP-2 gene spanned approximately 38 kb, consisted of four exons, and was similarly organized to that of the rat and human [5].
• Using primers from a conserved region of the mammalian IGFBP-2 sequence, a cDNA clone (1.6 kb) was isolated from an embryonic day-18 chick retina cDNA library [5].

Anatomical context of IGFBP2

• The results showed that the IGFBP2 SNP was associated with multiple traits, including BW, metatarsus length, shank length, femur length, shank weight, femur weight, metatarsus claw weight, and abdominal fat weight in the 1,028 NEAURP F2 individuals [3].
• Cloning and characterization of a chick embryo cDNA and gene for IGF-binding protein-2 [5].
• Consistent with this possibility, we found that exogenous IGFBP2 induces precocious expression of Indian hedgehog, a marker of prehypertrophy, in maturing chondrocytes in vitro [6].
• In contrast, IGFBP2 is present in the distal mesoderm of mutant limbless or wingless limb buds, which fail to grow out [6].
• IGF-I and IGFBP2 are also present in the osteoblasts, clasts and nascent matrix of the long bones, consistent with roles in endochondral bone formation [6].

Other interactions of IGFBP2

• The results indicate an increase in IGF-1 (P < 0.01) and a decrease in IGFBP-2 (P < 0.05) mRNA expression in slow muscle of the stimulated group compared with the control group [4].
• Roles of insulin-like growth factor-I (IGF-I) and IGF-I binding protein-2 (IGFBP2) and -5 (IGFBP5) in developing chick limbs [6].
• The IGF-II expression was not similar to IGFBP-2 during postnatal growth [7].
• Western blot analysis suggested that the 30 kDa IGFBP is homologous to IGFBP-2 found in mammalian blood plasma [8].

Analytical, diagnostic and therapeutic context of IGFBP2

• The PCR primers for the intron 2 region of IGFBP2 were designed based on chicken genomic sequence [3].
• At posthatch day 2 (P2), northern blot analyses of ocular tissues reveal that the cornea contains the highest ocular level of IGFBP-2 mRNA expression, a value equal to that of brain and liver [2].
• Feeding the low protein diet for 7 d decreased brain IGFBP-2 mRNA level and increased gizzard IGFBP-2 level compared with chickens fed 20% protein diets [1].

References