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Gene Review

EMC3  -  ER membrane protein complex subunit 3

Gallus gallus

Synonyms: POB, TMEM111
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Disease relevance of LOC415936

  • Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells [1].
  • The iron-saturated N-terminal half-molecule of ovotransferrin was proteolyzed into large 30-kDa (1-279) and small 6-kDa (280-332) fragments by a single cleavage with Achromobacter protease I, producing a stable nicked form [2].
  • The expressed ORF2 gene product had a molecular mass of 30 kDa, similar to that detected in purified virus particles [3].

High impact information on LOC415936

  • This was further confirmed using purified chicken gizzard vinculin, in that the 95-kDa vinculin head part, but not the 30-kDa tail part, directly bound to the alpha-domain portion [4].
  • The 30-kDa fragment containing the N-terminal hydrophobic region was produced by cell-free in vitro translation and found to assemble with canine pancreas rough microsomes [5].
  • In comparison, the natural metabolite 1alpha,25-dihydroxyvitamin D3-26,23-lactone yields only the 30-kDa fragment that is produced by all ligands to varying degrees [6].
  • The cellular distribution of three types of prosomes characterized by the presence of subunits with molecular masses of 23, 27, and 30 kDa was analyzed using an immunocytochemical approach on cultured chicken erythroblasts [7].
  • Together, these findings indicate that the 82 kDa macrogametocyte antigen, GAM82, is a tyrosine and serine rich precursor protein that is proteolytically processed during development to give rise to a 30 kDa protein, that is incorporated into the oocyst wall [8].

Biological context of LOC415936


Anatomical context of LOC415936


Associations of LOC415936 with chemical compounds


Physical interactions of LOC415936

  • The 30-kDa IGFBP was most affected by feed restriction with binding activity of this IGFBP increased by 2 days of feed restriction irrespective of the degree of feed deprivation [22].

Other interactions of LOC415936

  • By day 8 of incubation, IGF-1 levels were significantly higher and the 30 kDa IGFBP levels were significantly lower in the alcohol-treated group compared with vehicles [23].
  • First, both the purified 30-kDa tail fragment and a fusion protein containing tail domain, vinculin residues 884-1066, protect purified 95-kDa head domain from cleavage by protease V8 in a specific, dose-dependent manner [24].
  • The initial increase in binding activity observed with the 30-kDa IGFBP is similar to that observed with IGFBP-1 in mammals in that both of these IGFBPs respond rapidly to nutritional deprivation [25].
  • Chicken lipovitellin-2 is a small, approximately 30 kDa yolk protein, derived from the intracellular breakdown of the precursor protein vitellogenin [26].
  • Dephosphorylation of a 30-kDa protein of fowl spermatozoa by the addition of myosin light chain kinase substrate peptide inhibits the flagellar motility [10].

Analytical, diagnostic and therapeutic context of LOC415936

  • We were able to detect a protein fragment corresponding to the expected molecular mass (30 kDa) for type III deiodinase by means of Western blot analysis [27].
  • Following gel filtration of either extract, most of the protective antigens were confined to a single pool of proteins in the molecular mass range between 20 and 30 kDa [28].
  • Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 30 kDa [29].
  • The 30 kDa band also appeared at a lower intensity in the group fed a low protein diet but not in any other groups [11].
  • Multiple ligand-binding properties of the 30-kDa chicken alpha(1)-acid glycoprotein (cAGP), a member of the lipocalin protein family, were investigated for the first time by using circular dichroism (CD) and UV/Vis absorption spectroscopy methods [30].


  1. Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells. Janavs, J.L., Florez, J.C., Pierce, M.E., Takahashi, J.S. J. Neurosci. (1995) [Pubmed]
  2. Crucial role of intralobe peptide-peptide interactions in the uptake and release of iron by ovotransferrin. Kurokawa, H., Mikami, B., Hirose, M. J. Biol. Chem. (1994) [Pubmed]
  3. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. Nawagitgul, P., Morozov, I., Bolin, S.R., Harms, P.A., Sorden, S.D., Paul, P.S. J. Gen. Virol. (2000) [Pubmed]
  4. Functional analysis of Shigella VirG domains essential for interaction with vinculin and actin-based motility. Suzuki, T., Saga, S., Sasakawa, C. J. Biol. Chem. (1996) [Pubmed]
  5. Characterization of kinectin, a kinesin-binding protein: primary sequence and N-terminal topogenic signal analysis. Yu, H., Nicchitta, C.V., Kumar, J., Becker, M., Toyoshima, I., Sheetz, M.P. Mol. Biol. Cell (1995) [Pubmed]
  6. 25-Dehydro-1alpha-hydroxyvitamin D3-26,23S-lactone antagonizes the nuclear vitamin D receptor by mediating a unique noncovalent conformational change. Bula, C.M., Bishop, J.E., Ishizuka, S., Norman, A.W. Mol. Endocrinol. (2000) [Pubmed]
  7. In the nucleus and cytoplasm of chicken erythroleukemic cells, prosomes containing the p23K subunit are found in centers of globin (pre-)mRNA processing and accumulation. De Conto, F., Razin, S.V., Geraud, G., Arcangeletti, C., Scherrer, K. Exp. Cell Res. (1999) [Pubmed]
  8. Cloning and characterization of the 82 kDa tyrosine-rich sexual stage glycoprotein, GAM82, and its role in oocyst wall formation in the apicomplexan parasite, Eimeria maxima. Belli, S.I., Wallach, M.G., Smith, N.C. Gene (2003) [Pubmed]
  9. Cloning and analysis of calbindin-D28K cDNA and its expression in the central nervous system. Lomri, N., Perret, C., Gouhier, N., Thomasset, M. Gene (1989) [Pubmed]
  10. Dephosphorylation of a 30-kDa protein of fowl spermatozoa by the addition of myosin light chain kinase substrate peptide inhibits the flagellar motility. Ashizawa, K., Wishart, G.J., Hashimoto, K., Tsuzuki, Y. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
  11. Influence of nutrition on hepatic IGF-I mRNA levels and plasma concentrations of IGF-I and IGF-II in meat-type chickens. Kita, K., Tomas, F.M., Owens, P.C., Knowles, S.E., Forbes, B.E., Upton, Z., Hughes, R., Ballard, F.J. J. Endocrinol. (1996) [Pubmed]
  12. Apoptosis-induced concomitant release of cytosolic proteins and factors which prevent cell death. Endrich, M.M., Grossenbacher, D., Geistlich, A., Gehring, H. Biol. Cell (1996) [Pubmed]
  13. Bovine type XII collagen: amino acid sequence of a 10 kDa pepsin fragment from periodontal ligament reveals a high degree of homology with the chicken alpha 1(XII) sequence. Dublet, B., Dixon, E., de Miguel, E., van der Rest, M. FEBS Lett. (1988) [Pubmed]
  14. Inhibition of flagellar motility of fowl spermatozoa by L-carnitine: its relationship with respiration and phosphorylation of axonemal proteins. Ashizawa, K., Kamiya, T., Tamura, I., Tsuzuki, Y. Mol. Reprod. Dev. (1994) [Pubmed]
  15. A novel photopigment candidate in membranes of cultured chick pineal cells. Wallingford, J.C., Zatz, M. Exp. Eye Res. (1988) [Pubmed]
  16. Effect of fasting on serum insulin-like growth factor-I (IGF-I) levels and IGF-I-binding activity in cockerels. Morishita, D., Sasaki, K., Wakita, M., Hoshino, S. J. Endocrinol. (1993) [Pubmed]
  17. The 30 kDa abnormal protein in avian dystrophic muscle is indistinguishable from carbonic anhydrase III by physicochemical, immunological, and enzymological criteria. Kobayashi, R., Tashima, Y., Yoshioka, N. J. Biochem. (1990) [Pubmed]
  18. The presence of ATP + ubiquitin-dependent proteinase and multicatalytic proteinase complex in bovine brain. Azaryan, A., Banay-Schwartz, M., Lajtha, A. Neurochem. Res. (1989) [Pubmed]
  19. Subunit composition of the zinc proteins alpha- and beta-lipovitellin from chicken. Groche, D., Rashkovetsky, L.G., Falchuk, K.H., Auld, D.S. J. Protein Chem. (2000) [Pubmed]
  20. The 30 kDa protein co-purified with chick liver glutathione S-transferases is a carbonyl reductase. Tsai, S.P., Wang, L.Y., Yeh, H.I., Tam, M.F. Biochim. Biophys. Acta (1996) [Pubmed]
  21. Induction of specific proteins in cultured skeletal muscle cells by 1,25-dihydroxyvitamin D-3. Drittanti, L.N., Boland, R.L., de Boland, A.R. Biochim. Biophys. Acta (1989) [Pubmed]
  22. The effects of dietary restriction on insulin-like growth factor (IGF)-I and II, and IGF-binding proteins in chickens. Leili, S., Buonomo, F.C., Scanes, C.G. Proc. Soc. Exp. Biol. Med. (1997) [Pubmed]
  23. Alcohol-induced modulation of the insulin-like growth factor system in early chick embryo cranial tissue. Lynch, S.A., Elton, C.W., Carver, F.M., Pennington, S.N. Alcohol. Clin. Exp. Res. (2001) [Pubmed]
  24. An intramolecular association between the head and tail domains of vinculin modulates talin binding. Johnson, R.P., Craig, S.W. J. Biol. Chem. (1994) [Pubmed]
  25. The effects of protein restriction on insulin-like growth factor-I and IGF-binding proteins in chickens. Leili, S., Scanes, C.G. Proc. Soc. Exp. Biol. Med. (1998) [Pubmed]
  26. Active nuclear transport of chicken lipovitellin-2. Luo, Z., Annikka-Linnala-Kankkunen, n.u.l.l., Itkonen, A., Mäenpää, P.H. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  27. Specific detection of type III iodothyronine deiodinase protein in chicken cerebellar purkinje cells. Verhoelst, C.H., Vandenborne, K., Severi, T., Bakker, O., Zandieh Doulabi, B., Leonard, J.L., Kühn, E.R., van der Geyten, S., Darras, V.M. Endocrinology (2002) [Pubmed]
  28. Purification and characterization of a protective antigen from Eimeria tenella. Karkhanis, Y.D., Nollstadt, K.A., Bhogal, B.S., Ravino, O., Pellegrino, R., Crane, M.S., Murray, P.K., Turner, M.J. Infect. Immun. (1991) [Pubmed]
  29. Immunohistochemical studies of brain pyridoxine-5'-phosphate oxidase. Bahn, J.H., Kwon, O.S., Joo, H.M., Ho Jang, S., Park, J., Hwang, I.K., Kang, T.C., Won, M.H., Yil Kwon, H., Kwok, F., Kim, H.B., Cho, S.W., Choi, S.Y. Brain Res. (2002) [Pubmed]
  30. Multiple ligand-binding properties of the lipocalin member chicken alpha1-acid glycoprotein studied by circular dichroism and electronic absorption spectroscopy: the essential role of the conserved tryptophan residue. Zsila, F., Matsunaga, H., Bikádi, Z., Haginaka, J. Biochim. Biophys. Acta (2006) [Pubmed]
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