Disease relevance of LOC415936

• Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells [1].
• The iron-saturated N-terminal half-molecule of ovotransferrin was proteolyzed into large 30-kDa (1-279) and small 6-kDa (280-332) fragments by a single cleavage with Achromobacter protease I, producing a stable nicked form [2].
• The expressed ORF2 gene product had a molecular mass of 30 kDa, similar to that detected in purified virus particles [3].

High impact information on LOC415936

• This was further confirmed using purified chicken gizzard vinculin, in that the 95-kDa vinculin head part, but not the 30-kDa tail part, directly bound to the alpha-domain portion [4].
• The 30-kDa fragment containing the N-terminal hydrophobic region was produced by cell-free in vitro translation and found to assemble with canine pancreas rough microsomes [5].
• In comparison, the natural metabolite 1alpha,25-dihydroxyvitamin D3-26,23-lactone yields only the 30-kDa fragment that is produced by all ligands to varying degrees [6].
• The cellular distribution of three types of prosomes characterized by the presence of subunits with molecular masses of 23, 27, and 30 kDa was analyzed using an immunocytochemical approach on cultured chicken erythroblasts [7].
• Together, these findings indicate that the 82 kDa macrogametocyte antigen, GAM82, is a tyrosine and serine rich precursor protein that is proteolytically processed during development to give rise to a 30 kDa protein, that is incorporated into the oocyst wall [8].

Biological context of LOC415936

• This pC28 cDNA had an open reading frame (ORF) of 783 nucleotides (nt) coding for a 261-aa, 30-kDa protein [9].
• A marked difference in phosphorylation was observed in a 30 kDa protein [10].
• Both IGF-I and IGF-II ligands in the Western ligand blot revealed the most intense binding at 30 kDa for plasma obtained from chickens with restricted food intake [11].
• Conditioned medium (M(r) > or = 30 kDa) from serum-deprived cultures of chicken embryo fibroblasts completely prevented chicken embryo fibroblasts to undergo apoptosis as did phorbol 12-myristate, 13-acetate and, to -60%, L-cysteine [12].

Anatomical context of LOC415936

• A 10 kDa collagenous peptide, derived from a 30 kDa disulfide bonded fragment, was purified from bovine periodontal ligament [13].
• Phosphorylation of a 50 kDa protein and dephosphorylation of 24 kDa and 30 kDa proteins of demembranated spermatozoa were observed after the addition of carnitine [14].
• Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinealocyte membranes [15].
• Western blot analysis suggested that the 30 kDa IGFBP is homologous to IGFBP-2 found in mammalian blood plasma [16].
• Antibody to the 30 kDa protein cross-reacted with chicken skeletal muscle carbonic anhydrase III [17].

Associations of LOC415936 with chemical compounds

• All-trans-retinal, but not retinol or retinoic acid, incubated with pinealocyte homogenates prior to reduction, resulted in a loss of radioactivity from the 30 kDa region of the gel [15].
• A second proteinase was also found to be present (Mr 700 kDa) that precipitates at 38-80% ammonium sulfate saturation, is composed of multiple subunits ranging in Mr from 18 to 30 kDa, and degrades both protein and peptide substrates, demonstrating trypsin-, chymotrypsin- and cucumisin-like activities [18].
• The subunits of beta-lipovitellin, Lv 1 (MW 125 kDa) and Lv 2 (MW 30 kDa), are separated by gel exclusion chromatography in the presence of zwittergent 3-16 [19].
• An unidentified 30 kDa protein was co-purified with chick liver glutathione S-transferases from S-hexylglutathione affinity column [20].
• Examination of 3H/14C ratios in gel fractions revealed that 1,25-(OH)2D3 stimulates the production of proteins of molecular masses (isoelectric points) of 9 kDa (4.1 and 8.5), 17 kDa (7.5), 30 kDa (7.2), 40 kDa (5.5), 55 kDa (4.5) and 100 kDa (8.6) [21].

Physical interactions of LOC415936

• The 30-kDa IGFBP was most affected by feed restriction with binding activity of this IGFBP increased by 2 days of feed restriction irrespective of the degree of feed deprivation [22].

Other interactions of LOC415936

• By day 8 of incubation, IGF-1 levels were significantly higher and the 30 kDa IGFBP levels were significantly lower in the alcohol-treated group compared with vehicles [23].
• First, both the purified 30-kDa tail fragment and a fusion protein containing tail domain, vinculin residues 884-1066, protect purified 95-kDa head domain from cleavage by protease V8 in a specific, dose-dependent manner [24].
• The initial increase in binding activity observed with the 30-kDa IGFBP is similar to that observed with IGFBP-1 in mammals in that both of these IGFBPs respond rapidly to nutritional deprivation [25].
• Chicken lipovitellin-2 is a small, approximately 30 kDa yolk protein, derived from the intracellular breakdown of the precursor protein vitellogenin [26].
• Dephosphorylation of a 30-kDa protein of fowl spermatozoa by the addition of myosin light chain kinase substrate peptide inhibits the flagellar motility [10].

Analytical, diagnostic and therapeutic context of LOC415936

• We were able to detect a protein fragment corresponding to the expected molecular mass (30 kDa) for type III deiodinase by means of Western blot analysis [27].
• Following gel filtration of either extract, most of the protective antigens were confined to a single pool of proteins in the molecular mass range between 20 and 30 kDa [28].
• Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 30 kDa [29].
• The 30 kDa band also appeared at a lower intensity in the group fed a low protein diet but not in any other groups [11].
• Multiple ligand-binding properties of the 30-kDa chicken alpha(1)-acid glycoprotein (cAGP), a member of the lipocalin protein family, were investigated for the first time by using circular dichroism (CD) and UV/Vis absorption spectroscopy methods [30].

References