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Gene Review

Ca-P60A  -  Calcium ATPase at 60A

Drosophila melanogaster

Synonyms: A4UZU0_DROME, ATC1_DROME, CA-P60A, CG3725, Ca-ATPase, ...
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High impact information on Ca-P60A

  • We identified a lethal complementation group recovered in a screen for mutations that reduce Notch activity as loss-of-function alleles of the Drosophila Ca(2+)-ATPase gene Ca-P60A [1].
  • Notch and several other transmembrane proteins are mislocalized in tissue clones homozygous for Ca-P60A mutations, demonstrating a general effect on membrane protein trafficking caused by a deficiency in Ca(2+)-ATPase [1].
  • Analysis of Ca-P60A mutants indicates that Ca(2+)-ATPase is essential for cell viability and tissue morphogenesis during development [1].
  • Maximum parsimony and Neighbour Joining were used to generate a phylogenetic classification of Drosophila ryanodine and insitol triphosphate receptors and Ca2+-ATPase based on 48 invertebrate and vertebrate complete sequences [2].
  • Despite evolutionary distances, our functional results demonstrate that Drosophila ryanodine and inositol triphosphate receptors and Ca2+-ATPase are reasonably similar to vertebrate counterparts [2].

Biological context of Ca-P60A


Anatomical context of Ca-P60A

  • SERCA, a sarco-endoplasmic reticulum calcium ATPase, being the main agent for calcium uptake into the ER, plays a central role in this process [3].
  • In Ca2+-free medium, the endoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin, elevates [Ca2+]i only in the smaller stellate cells, suggesting that principal cells do not contain a thapsigargin-sensitive intracellular pool [6].
  • Following the sarcoplasmic reticulum (SR) Ca(2+) store depletion by thapsigargin (Tg), a SERCA inhibitor, OAG and PHE were both still able to activate Ca(2+) influx [7].
  • A combination of RNAi and calcium imaging has provided a direct demonstration of key roles for the Ins(1,4,5)P(3)R and the SERCA pump in the response to DM1 receptor activation.Thus, we show that silencing of individual genes by RNAi in a well characterised Drosophila S2 cell line offers experimental opportunities for cell-signalling studies [8].
  • Like its relative in Drosophila, TRPC1 looks likely to function in a signalplex, a protein complex including inositol 1,4,5-triphosphate (IP(3)) receptor, plasma membrane calcium-ATPase, caveolin-1 and calmodulin [9].

Associations of Ca-P60A with chemical compounds

  • We have shown that RNAi knock down of either the inositol 1,4,5-trisphosphate receptor (Ins(1,4,5)P(3)R), or the SERCA calcium pump in the S2-DM1 cells blocks the increase in intracellular calcium concentration ([Ca(2+)](i)) resulting from activation of the DM1 receptor by 100 microM carbamylcholine (CCh) [8].

Other interactions of Ca-P60A

  • Analysis of conditional paralytic mutants in Drosophila sarco-endoplasmic reticulum calcium ATPase reveals novel mechanisms for regulating membrane excitability [3].
  • Bursts of neural activity induced in Drosophila comatosets and CaP60A Kumts mutants, with conditional defects in N-ethylmaleimide-sensitive fusion factor 1 and sarco-endoplasmic reticulum Ca2+ ATPase, respectively, result in persistent (>4 hr) activation of neuronal extracellular signal-regulated kinase (ERK) [10].

Analytical, diagnostic and therapeutic context of Ca-P60A


  1. Ca(2+)-ATPase function is required for intracellular trafficking of the Notch receptor in Drosophila. Periz, G., Fortini, M.E. EMBO J. (1999) [Pubmed]
  2. Biochemical characterization, distribution and phylogenetic analysis of Drosophila melanogaster ryanodine and IP3 receptors, and thapsigargin-sensitive Ca2+ ATPase. Vázquez-Martínez, O., Cañedo-Merino, R., Díaz-Muñoz, M., Riesgo-Escovar, J.R. J. Cell. Sci. (2003) [Pubmed]
  3. Analysis of conditional paralytic mutants in Drosophila sarco-endoplasmic reticulum calcium ATPase reveals novel mechanisms for regulating membrane excitability. Sanyal, S., Consoulas, C., Kuromi, H., Basole, A., Mukai, L., Kidokoro, Y., Krishnan, K.S., Ramaswami, M. Genetics (2005) [Pubmed]
  4. Cloning and characterization of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) from crayfish axial muscle. Sarco/Endoplasmic Reticulum Ca(2+)-ATPase. Zhang, Z., Chen, D., Wheatly, M.G. J. Exp. Biol. (2000) [Pubmed]
  5. Conditional mutations in SERCA, the Sarco-endoplasmic reticulum Ca(2+)-ATPase, alter heart rate and rhythmicity in Drosophila. Sanyal, S., Jennings, T., Dowse, H., Ramaswami, M. J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. (2006) [Pubmed]
  6. Cell-type specific calcium signalling in a Drosophila epithelium. Rosay, P., Davies, S.A., Yu, Y., Sözen, A., Kaiser, K., Dow, J.A. J. Cell. Sci. (1997) [Pubmed]
  7. Receptor-operated Ca2+ entry mediated by TRPC3/TRPC6 proteins in rat prostate smooth muscle (PS1) cell line. Thebault, S., Zholos, A., Enfissi, A., Slomianny, C., Dewailly, E., Roudbaraki, M., Parys, J., Prevarskaya, N. J. Cell. Physiol. (2005) [Pubmed]
  8. Gene silencing of selected calcium-signalling molecules in a Drosophila cell line using double-stranded RNA interference. Raymond-Delpech, V., Towers, P.R., Sattelle, D.B. Cell Calcium (2004) [Pubmed]
  9. TRPC1 store-operated cationic channel subunit. Beech, D.J., Xu, S.Z., McHugh, D., Flemming, R. Cell Calcium (2003) [Pubmed]
  10. Acute induction of conserved synaptic signaling pathways in Drosophila melanogaster. Hoeffer, C.A., Sanyal, S., Ramaswami, M. J. Neurosci. (2003) [Pubmed]
  11. Amino acid sequence of a Ca(2+)-transporting ATPase from the sarcoplasmic reticulum of the cross-striated part of the adductor muscle of the deep sea scallop: comparison to serca enzymes of other animals. Shi, X., Chen, M., Huvos, P.E., Hardwicke, P.M. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (1998) [Pubmed]
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