The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Atp2a1  -  ATPase, Ca++ transporting, cardiac muscle,...

Rattus norvegicus

Synonyms: Calcium pump 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, SERCA1, SR Ca(2+)-ATPase 1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Atp2a1

  • Myocytes from banded hearts treated with T4 were hypertrophied but had increased concentrations of alpha-MHC and SERCA proteins, similar to physiological hypertrophy induced by exercise [1].
  • 1. Sarco-endoplasmic reticulum Ca2+-ATPase from fast skeletal (SERCA1) or cardiac muscle (SERCA2a) was expressed in embryonic chicken and neonatal rat cardiac myocytes by adenovirus vectors, with c-myc tags on both constructs to compare expression and distinguish exogenous from endogenous SERCA2a in myocytes [2].
  • We investigated the effects of graded levels of low-flow ischemia on myocardial function and on SR Ca(2+)-ATPase (SERCA2), Na(+)-Ca2+ exchanger (NCX) and ryanodine receptor (RyR2), at mRNA and protein levels in both adult and senescent myocardium [3].
  • Depressed PKA activity contributes to impaired SERCA function and is linked to the pathogenesis of glucose-induced cardiomyopathy [4].
  • Paraplegia significantly increased the relative protein abundances of SERCA (45%) and the Na/Ca exchanger (40%) and decreased phospholamban levels (-28%) [5].

Psychiatry related information on Atp2a1


High impact information on Atp2a1


Chemical compound and disease context of Atp2a1


Biological context of Atp2a1

  • Nevertheless, the kinetics properties of the brown fat SERCA differ from the skeletal muscle SERCA 1 inasmuch they manifest a different Ca2+ affinity and a much higher degree of ATPase/Ca2+ uncoupling [16].
  • We conclude that upregulation of SERCA1 protein and Ca(2+)-ATPase activity may be an adaptive mechanism and/or a contributory process in the pathology of alcohol-induced muscle disease [17].
  • With use of in vivo plasmid injection, the activity of a reporter gene driven by 3.6 kb of the SERCA1 5'-flanking region increased fivefold in 7-day-unloaded soleus muscles [18].
  • However, because of the difficulty of measuring transcription rates from whole muscle, transcriptional activation of the SERCA1 gene with unloading has not been confirmed [18].
  • SERCA2, the cardiac/slow-twitch skeletal muscle isoform, was not markedly increased by unloading, and RNase protection assays showed no change in alternative splicing of SERCA1 or SERCA2 primary transcripts [18].

Anatomical context of Atp2a1

  • In this report a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) was identified in rats brown adipose tissue [16].
  • This study examined whether HSP70 could bind to and protect against thermal inactivation of SERCA1a, the SERCA isoform expressed in adult fast-twitch skeletal muscle [19].
  • In addition, hearts freeze-clamped at various times postburn (2, 4, 8, and 24 h) were used for Western blot analysis using antibodies against the sarcoplasmic reticulum calcium-ATPase (SERCA), the L-type calcium-channel, the ryanodine receptor, the sodium/calcium exchanger, or the sodium-potassium-ATPase [20].
  • Immunofluorescence labeling of sheep Purkinje fibers show that the ryanodine receptor is confined to discrete foci while the SR-Ca(2+)-ATPase is distributed in a continuous network-like structure present at the periphery as well as throughout interior regions of these myofibers [10].
  • Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) gene silencing and remodeling of the Ca2+ signaling mechanism in cardiac myocytes [21].

Associations of Atp2a1 with chemical compounds

  • HSP70 also protected against reductions in binding capacity for fluorescein isothiocyanate, a fluorescent probe that binds to Lys515 in the nucleotide binding domain of SERCA, at 30 and 60 min but not at 120 min, an effect that was independent of temperature [19].
  • In intact cells, the actions of XeD are blocked by ryanodine pretreatment and do not interfere with thapsigargin-mediated Ca(2+) mobilization stemming from SERCA block [22].
  • We have further studied the specificity of xestospongin structures possessing ring hydroxyl (-OH) substituents toward IP(3)R, RyR, and ER/SR Ca(2+)-ATPase (SERCA) activities [22].
  • 2. Triamcinolone treatment reduced the normalised overall amount of all SERCA mRNA in diaphragm by 70 % compared to controls (P < 0.05) [23].
  • The increased SR-Ca(2+)-ATPase/phospholamban ratio and decrease in phospholamban protein content in T3-treated cells was reflected in a parallel increase of contraction and calcium transients and more rapid Ca2+ reuptake, but the post-rest potentiation and response to isoproterenol were reduced [24].

Enzymatic interactions of Atp2a1

  • In hypertrophic hearts, quantitative immunoblotting analyses showed increased levels both of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) and phosphorylated phospholamban, along with decreased levels of total phospholamban, which is in line with strengthened right ventricular systolic function [25].

Regulatory relationships of Atp2a1


Other interactions of Atp2a1

  • XeC potently inhibits IP(3)R, weakly inhibits RyR1, and lacks activity toward SERCA1 and SERCA2 [22].
  • Phospholamban protein content was significantly reduced by 33% but SR-Ca(2+)-ATPase protein content was not significantly altered in T3-cells [24].
  • Corticosteroids decrease mRNA levels of SERCA pumps, whereas they increase sarcolipin mRNA in the rat diaphragm [23].
  • Sarco(endo)plasmic reticulum Ca2+ pumps are encoded by genes SERCA1, SERCA2, and SERCA3 [30].
  • ER refilling was less complete in PDBu-treated cells, although, in AS4-expressing cells, PDBu accelerated the rate without reducing the ER capacity, suggesting that PMCA and SERCA compete for Ca2+ [31].

Analytical, diagnostic and therapeutic context of Atp2a1


  1. Thyroid hormone improves function and Ca2+ handling in pressure overload hypertrophy. Association with increased sarcoplasmic reticulum Ca2+-ATPase and alpha-myosin heavy chain in rat hearts. Chang, K.C., Figueredo, V.M., Schreur, J.H., Kariya, K., Weiner, M.W., Simpson, P.C., Camacho, S.A. J. Clin. Invest. (1997) [Pubmed]
  2. Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes. Cavagna, M., O'Donnell, J.M., Sumbilla, C., Inesi, G., Klein, M.G. J. Physiol. (Lond.) (2000) [Pubmed]
  3. Effects of sustained low-flow ischemia on myocardial function and calcium-regulating proteins in adult and senescent rat hearts. Assayag, P., CHarlemagne, D., Marty, I., de Leiris, J., Lompré, A.M., Boucher, F., Valére, P.E., Lortet, S., Swynghedauw, B., Besse, S. Cardiovasc. Res. (1998) [Pubmed]
  4. Depressed PKA activity contributes to impaired SERCA function and is linked to the pathogenesis of glucose-induced cardiomyopathy. Dutta, K., Carmody, M.W., Cala, S.E., Davidoff, A.J. J. Mol. Cell. Cardiol. (2002) [Pubmed]
  5. Increased susceptibility to ventricular arrhythmias is associated with changes in Ca2+ regulatory proteins in paraplegic rats. Rodenbaugh, D.W., Collins, H.L., Nowacek, D.G., DiCarlo, S.E. Am. J. Physiol. Heart Circ. Physiol. (2003) [Pubmed]
  6. Different aspects of the effects of thapsigargin on automatism, contractility and responsiveness to phenylephrine in cardiac preparations from rats and guinea pigs. Kocic, I., Dworakowska, D., Dworakowski, R. Pharmacol. Res. (1998) [Pubmed]
  7. Ca2+-dependent redox modulation of SERCA 2b by ERp57. Li, Y., Camacho, P. J. Cell Biol. (2004) [Pubmed]
  8. Ankyrin-B is required for intracellular sorting of structurally diverse Ca2+ homeostasis proteins. Tuvia, S., Buhusi, M., Davis, L., Reedy, M., Bennett, V. J. Cell Biol. (1999) [Pubmed]
  9. Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi. Lin, P., Yao, Y., Hofmeister, R., Tsien, R.Y., Farquhar, M.G. J. Cell Biol. (1999) [Pubmed]
  10. The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle. Jorgensen, A.O., Shen, A.C., Arnold, W., McPherson, P.S., Campbell, K.P. J. Cell Biol. (1993) [Pubmed]
  11. Angiotensin type 1 receptor antagonism with irbesartan inhibits ventricular hypertrophy and improves diastolic function in the remodeling post-myocardial infarction ventricle. Ambrose, J., Pribnow, D.G., Giraud, G.D., Perkins, K.D., Muldoon, L., Greenberg, B.H. J. Cardiovasc. Pharmacol. (1999) [Pubmed]
  12. Left ventricular wall stress and sarcoplasmic reticulum Ca(2+)-ATPase gene expression in renal hypertensive rats: dose-dependent effects of ACE inhibition and AT1-receptor blockade. Zierhut, W., Studer, R., Laurent, D., Kästner, S., Allegrini, P., Whitebread, S., Cumin, F., Baum, H.P., de Gasparo, M., Drexler, H. Cardiovasc. Res. (1996) [Pubmed]
  13. Apoptosis of insulin-secreting cells induced by endoplasmic reticulum stress is amplified by overexpression of group VIA calcium-independent phospholipase A2 (iPLA2 beta) and suppressed by inhibition of iPLA2 beta. Ramanadham, S., Hsu, F.F., Zhang, S., Jin, C., Bohrer, A., Song, H., Bao, S., Ma, Z., Turk, J. Biochemistry (2004) [Pubmed]
  14. Dronedarone administration prevents body weight gain and increases tolerance of the heart to ischemic stress: a possible involvement of thyroid hormone receptor alpha1. Pantos, C., Mourouzis, I., Malliopoulou, V., Paizis, I., Tzeis, S., Moraitis, P., Sfakianoudis, K., Varonos, D.D., Cokkinos, D.V. Thyroid (2005) [Pubmed]
  15. Differentiation of answer of glioma C6 cells to SERCA pump inhibitors by actin disorganization. Supłat, D., Targos, B., Sabała, P., Barańska, J., Pomorski, P. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  16. Brown adipose tissue Ca2+-ATPase: uncoupled ATP hydrolysis and thermogenic activity. de Meis, L. J. Biol. Chem. (2003) [Pubmed]
  17. Ca2+-regulatory muscle proteins in the alcohol-fed rat. Ohlendieck, K., Harmon, S., Koll, M., Paice, A.G., Preedy, V.R. Metab. Clin. Exp. (2003) [Pubmed]
  18. Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle. Peters, D.G., Mitchell-Felton, H., Kandarian, S.C. Am. J. Physiol. (1999) [Pubmed]
  19. HSP70 binds to the fast-twitch skeletal muscle sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA1a) and prevents thermal inactivation. Tupling, A.R., Gramolini, A.O., Duhamel, T.A., Kondo, H., Asahi, M., Tsuchiya, S.C., Borrelli, M.J., Lepock, J.R., Otsu, K., Hori, M., MacLennan, D.H., Green, H.J. J. Biol. Chem. (2004) [Pubmed]
  20. Burn trauma alters calcium transporter protein expression in the heart. Ballard-Croft, C., Carlson, D., Maass, D.L., Horton, J.W. J. Appl. Physiol. (2004) [Pubmed]
  21. Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) gene silencing and remodeling of the Ca2+ signaling mechanism in cardiac myocytes. Seth, M., Sumbilla, C., Mullen, S.P., Lewis, D., Klein, M.G., Hussain, A., Soboloff, J., Gill, D.L., Inesi, G. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  22. Hydroxylated xestospongins block inositol-1,4,5-trisphosphate-induced Ca2+ release and sensitize Ca2+-induced Ca2+ release mediated by ryanodine receptors. Ta, T.A., Feng, W., Molinski, T.F., Pessah, I.N. Mol. Pharmacol. (2006) [Pubmed]
  23. Corticosteroids decrease mRNA levels of SERCA pumps, whereas they increase sarcolipin mRNA in the rat diaphragm. Gayan-Ramirez, G., Vanzeir, L., Wuytack, F., Decramer, M. J. Physiol. (Lond.) (2000) [Pubmed]
  24. Thyroid hormone control of contraction and the Ca(2+)-ATPase/phospholamban complex in adult rat ventricular myocytes. Holt, E., Sjaastad, I., Lunde, P.K., Christensen, G., Sejersted, O.M. J. Mol. Cell. Cardiol. (1999) [Pubmed]
  25. Effect of endothelin antagonism on contractility, intracellular calcium regulation and calcium regulatory protein expression in right ventricular hypertrophy of the rat. Stessel, H., Brunner, F. Basic & clinical pharmacology & toxicology. (2004) [Pubmed]
  26. Adenoviral gene transfer of phospholamban in isolated rat cardiomyocytes. Rescue effects by concomitant gene transfer of sarcoplasmic reticulum Ca(2+)-ATPase. Hajjar, R.J., Schmidt, U., Kang, J.X., Matsui, T., Rosenzweig, A. Circ. Res. (1997) [Pubmed]
  27. Purkinje neurons express the SERCA3 isoform of the organellar type Ca(2+)-transport ATPase. Baba-Aïssa, F., Raeymaekers, L., Wuytack, F., Callewaert, G., Dode, L., Missiaen, L., Casteels, R. Brain Res. Mol. Brain Res. (1996) [Pubmed]
  28. IGF-I attenuates diabetes-induced cardiac contractile dysfunction in ventricular myocytes. Norby, F.L., Wold, L.E., Duan, J., Hintz, K.K., Ren, J. Am. J. Physiol. Endocrinol. Metab. (2002) [Pubmed]
  29. Interleukin-6-induced reciprocal expression of SERCA and natriuretic peptides mRNA in cultured rat ventricular myocytes. Tanaka, T., Kanda, T., Takahashi, T., Saegusa, S., Moriya, J., Kurabayashi, M. J. Int. Med. Res. (2004) [Pubmed]
  30. Sarco(endo)plasmic reticulum Ca2+ pump isoform SERCA3 is more resistant than SERCA2b to peroxide. Grover, A.K., Samson, S.E., Misquitta, C.M. Am. J. Physiol. (1997) [Pubmed]
  31. Activation of protein kinase C in sensory neurons accelerates Ca2+ uptake into the endoplasmic reticulum. Usachev, Y.M., Marsh, A.J., Johanns, T.M., Lemke, M.M., Thayer, S.A. J. Neurosci. (2006) [Pubmed]
  32. Oxidation of sarcoplasmic reticulum Ca(2+)-ATPase induced by high-intensity exercise. Matsunaga, S., Inashima, S., Yamada, T., Watanabe, H., Hazama, T., Wada, M. Pflugers Arch. (2003) [Pubmed]
  33. Expression of sarcoplasmic/endoplasmic reticulum Ca2+ ATPases in the rat extensor digitorum longus (EDL) muscle regenerating from notexin-induced necrosis. Mendler, L., Szakonyi, G., Zádor, E., Görbe, A., Dux, L., Wuytack, F. J. Muscle Res. Cell. Motil. (1998) [Pubmed]
  34. Insulin receptor substrate proteins create a link between the tyrosine phosphorylation cascade and the Ca2+-ATPases in muscle and heart. Algenstaedt, P., Antonetti, D.A., Yaffe, M.B., Kahn, C.R. J. Biol. Chem. (1997) [Pubmed]
  35. Cellular distribution of Ca2+ pumps and Ca2+ release channels in rat cardiac hypertrophy induced by aortic stenosis. Anger, M., Lompré, A.M., Vallot, O., Marotte, F., Rappaport, L., Samuel, J.L. Circulation (1998) [Pubmed]
WikiGenes - Universities