Disease relevance of DPM3

• The hemodynamic and oxygen transport effects of prostaglandin E1 (PGE1, Prostin -VR), in increasing doses from 1 to 30 ng/kg X min were evaluated in 5 severely ill surgical patients with adult respiratory distress syndrome (ARDS) [1].
• A 22-month experience treating postpartum hemorrhage with Prostin 15M patients who had not responded to conventional therapy is presented [2].

High impact information on DPM3

• The third subunit, DPM3, comprises 92 amino acids associated with DPM1 via its C-terminal domain and with DPM2 via its N-terminal portion [3].
• The stability of DPM3 was dependent upon DPM2 [3].
• A coiled-coil domain near the C terminus of DPM3 was important for tethering DPM1, the catalytic subunit of the enzyme, to the endoplasmic reticulum membrane and, therefore, was critical for enzyme activity [4].
• CHO2.38 cells were negative for GPI-anchored proteins, and microsomes from these cells showed no detectable DPM synthase activity, indicating that DPM3 is an essential component of this enzyme [4].
• The prostin-1 gene, conserved through phylogeny, is induced by androgen in LNCaP cells and encodes a 92 amino acid protein [5].

Chemical compound and disease context of DPM3

• The use of 15 methyl F2 alpha prostaglandin (Prostin 15M) for the control of postpartum hemorrhage [2].

Biological context of DPM3

• That DPM3/prostin-1 might suppress tumor progression was supported by the finding that exogenous expression in COS cells leads to apoptosis [5].
• Induction of abortion in the second trimester of pregnancy by intramuscular 15-methyl prostaglandin F 2 alpha (Prostin 15M) [6].
• The use of intramuscular (15S)-15-methyl prostaglandin F2 alpha (Prostin/15 M [The Upjohn Company, Kalamazoo, Michigan] sterile solution; carboprost tromethamine) for termination of pregnancy between six and 24 weeks' gestation was studied by 20 investigators [7].

Associations of DPM3 with chemical compounds

• These results indicate that prostin may be a physiological activator of pro-PSA following its own proteolytic activation, as part of a cascade system involving a series of serine protease precursor proteins in the prostate [8].
• Prostaglandin E1 (alprostadil, Prostin VR Sterile Solution, PGE1) was evaluated in patients with Raynaud's syndrome in a multiclinic, placebo-controlled, double-blind study [9].
• An adsorption high-performance liquid chromatographic method suitable for the quantitative determination of the purity of alprostadil (prostaglandin E1) bulk drug and of the concentration of alprostadil in Prostin V.R. Pediatric Sterile Solution is described [10].
• Blood replacement, oxytocin, and ergot therapy along with manual reduction failed to prevent immediate recurrence, but treatment with 15(S)-15-methyl prostaglandin F2 alpha (Prostin 15M) and uterine packing were successful [11].
• A randomized comparative study of 387 consecutive patients admitted for induction of labor was carried out using two orally administered oxytocics (prostaglandin E2 tablets (Prostin) or Demoxytocin resoriblets for buccal administration (Sandopart)), the results of which are reported here [12].

Regulatory relationships of DPM3

• Recombinant prostin readily activates the precursor of PSA (pro-PSA) by cleavage of the amino terminal Arg(7)-Ile(8) peptide bond [8].

Other interactions of DPM3

• The zymogen, with a molecular mass of 28 kDa, was readily activated by agarose-immobilized trypsin to generate prostin, a serine protease, which cleaves the chromogenic substrate (N-benzoyl-L-Ile-L-Glu-L-Gly-L-Arg-p-nitroaniline hydrochloride) (S-2222) [8].
• Significant relationships were observed inversely between the total prostin dose and Bishop score (R(2)=0.33; P=0.002), between the total prostin dose and latent phase (R(2)=0.54; P=0.000009) and between Bishop score and the fibronectin levels (R(2)=0.19; P<0.01) [13].

Analytical, diagnostic and therapeutic context of DPM3

• Activation of prostate-specific antigen precursor (pro-PSA) by prostin, a novel human prostatic serine protease identified by degenerate PCR [8].
• An absorption high-performance liquid chromatography assay for the quantitative determination of prostaglandins A1 and B1 in alprostadil (prostaglandin E1) and in Prostin VR Pediatric Sterile Solution was developed [14].
• A 41-year old primigravida underwent caesarean section because of foetal distress following prostin induction of labour [15].
• Slow release prostaglandin pessary (propess) is compared with instant release prostaglandin gel (prostin) for the induction of labour in nulliparous women with a modified Bishop's score of less than 6 [16].

References