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Gene Review

GOSR1  -  golgi SNAP receptor complex member 1

Homo sapiens

Synonyms: 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOLIM2, GOS-28, GOS28, ...
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Disease relevance of GOSR1


Psychiatry related information on GOSR1

  • Sensory neglect and apomorphine-induced rotation were measured at P27 and P28 [6].
  • The present experiments explored social consequences of ethanol during adolescence by examining dose-dependent ethanol-induced social facilitation and inhibition in a non-anxiogenic (familiar) environment, and ethanol-related anxiolysis in an anxiogenic (unfamiliar) environment in early (P28) and late (P42) adolescent rats [7].

High impact information on GOSR1

  • Both mu- and m-calpain are heterodimers containing an identical 28-kDa subunit and an 80-kDa subunit that shares 55-65% sequence homology between the two proteases [8].
  • The transfer may be facilitated by the 28-kDa water channel-forming integral protein (CHIP28), which is expressed in the trophoblast syncytium [9].
  • Taspase1 proenzyme is intramolecularly proteolyzed generating an active 28 kDa alpha/22 kDa beta heterodimer [10].
  • P0 is a 28 kDa glycoprotein involved in the compaction of the multilamellar myelin sheet and accounts for more than half of the peripheral myelin protein content [11].
  • The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy [12].

Chemical compound and disease context of GOSR1

  • Pseudomonas exotoxin (PE) enters cells by receptor-mediated endocytosis and is cleaved by a cellular protease between Arg279 and Gly280 to produce an NH2-terminal fragment of 28 kDa which contains the toxin's binding domain and a COOH-terminal fragment of 37 kDa which has translocating and ADP-ribosylating activity [13].
  • We have previously reported the presence of a 28-kDa protein in human mammary adenocarcinoma MCF-7 cells, whose phosphorylation by phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and permeant diacylglycerol 1,2-dioctanoyl-sn-glycerol was correlated to growth arrest induced by the protein kinase C (PKC) activators [14].
  • In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase [15].
  • Herpesviruses encode an essential, maturational serine protease whose catalytic domain, assemblin (28 kDa), is released by self-cleavage from a 74-kDa precursor (pPR, pUL80a) [16].
  • This paper reports the crystal structure of the Schistosoma haematobium 28 kDa glutathione S-transferase, a multifunctional enzyme involved in host-parasite interactions and presently considered as a promising vaccine candidate against schistosomiasis [17].

Biological context of GOSR1

  • cDNA characterization and chromosomal mapping of human golgi SNARE GS27 and GS28 to chromosome 17 [18].
  • The N terminus of the deduced amino acid sequence had properties characteristic for a mitochondrial translocation signal, and cleavage at a putative mitochondrial peptidase cleavage site would give a mature protein size of 28 kDa [19].
  • Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells [20].
  • Antibodies 232 and 233, specific for synthetic peptides pep14-24 and pep117-127, corresponding to two nonoverlapping HTLV-related regions in the longer open reading frame of HRES-1, recognize an identical 28-kDa protein in H9 human T cells [21].
  • From a flounder pituitary cDNA library, cDNA clones encoding a 28-kDa glycoprotein produced by the pars intermedia of the pituitary were isolated and characterized [22].

Anatomical context of GOSR1

  • We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks [23].
  • Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles [23].
  • Water-permeable membranes of several plant and mammalian tissues contain specific water channel proteins, the 'aquaporins'. The best characterized aquaporin is CHIP, a 28 kDa red blood cell channel-forming integral protein [24].
  • By using human alpha2 laminins as a probe, a major 28-kDa protein in the M. leprae cell wall fraction that binds alpha2 laminins was identified [25].
  • CD52 is a 21- to 28-kDa nonmodulating cell surface glycosylphosphatidylinositol-linked glycoprotein expressed on lymphocytes and monocytes, but not in human myeloid cells [26].

Associations of GOSR1 with chemical compounds

  • We demonstrate here that the 28 kDa I-TevI binds the extensive 37 bp td homing site as a monomer and significantly distorts its substrate [27].
  • BF is a protein with a molecular mass of 28 kDa on reducing sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) [28].
  • Phenobarbitone treatment of the animal leads to a significant increase in the phosphorylation of the 26- to 28-kDa and 94-kDa proteins in nuclear labeling experiments followed by isolation on a PE affinity column [29].
  • The resultant 28-kDa EBNA fusion polypeptide, comprising 5-10% of the total soluble bacterial protein, was purified to apparent homogeneity by phosphocellulose and hydroxylapatite column chromatography [30].
  • The three other proteins, of 100, 32, and 28 kDa, display little sequence or structural binding specificity apart from their preference for uridine-rich sequences [31].

Physical interactions of GOSR1

  • The structure of the 28 kDa beta-lactamase inhibitor protein-II (BLIP-II) in complex with the TEM-1 beta-lactamase has been determined to 2.3 A resolution [32].
  • Several lines of evidence indicate that the 28-kDa and the 26-kDa cap binding proteins of eIF-4B and eIF-4F are antigenically distinct polypeptides [33].
  • Using an anti-clusterin affinity column, we found that an additional protein component with a molecular mass of 28-kDa co-purifies with clusterin from human plasma [34].
  • The homology observed suggests, after comparison with the structures of other intracellular CaBPs, that rat 9 kDa CaBP mRNA contains the remains of an untranslated calcium-binding site III-like structure seen in 28 kDa CaBP from kidney and cerebellum of rat [35].
  • In the artery extract, serum IgG from 6/18 MPA patients bound to an 85 kDa antigen, whereas purified IgG from all WG patients tested bound to a 28 kDa protein band and IgM from CSS patients bound to 2 main antigens of 38 and 60 kDa [36].

Enzymatic interactions of GOSR1

  • RPA32 was cleaved rapidly to a approximately 28-kDa polypeptide containing the C-terminus that was partially resistant to further digestion [37].
  • Furthermore, Western blots showed a 118-kDa STAT1 band at the start of the PHA stimulation that was apparently cleaved to STAT1alpha (91 kDa) and a 28 kDa-fragment with further stimulation [38].
  • PS1 protein is generated as a 47 kDa protein and is endoproteolytically cleaved into N-terminal 28 kDa and C-terminal 19 kDa fragments in vivo [39].
  • MMP-7 cleaves the collagen XVIII NC1 domain to generate a 28-kDa fragment in the cornea [40].

Regulatory relationships of GOSR1


Other interactions of GOSR1

  • Chromosomal mapping analyses reveal that human GS27 is located on chromosome 17q21 and GS28 on approximately 17q11 [18].
  • We also report that GOS 28 and its partnering t-SNARE heavy chain, syntaxin 5, reside together in every cisterna of the stack [1].
  • Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) GS28 and syntaxin 5 can be reciprocally coimmunoprecipitated from Golgi extracts, suggesting that they exist in a protein complex [45].
  • Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)/mass-spectrometry analysis identified the 28-kDa protein as human tumor protein D52 (TPD52), whose expression had been previously described only in normal and neoplastic epithelia [46].
  • These data suggest that 28-kDa pro-BDNF is not an obligatory intermediate in the formation of the 14-kDa form in the constitutive secretory pathway [47].

Analytical, diagnostic and therapeutic context of GOSR1

  • In agreement with this result and also suggesting that the native protein is a monomer, gel-filtration experiments indicated a molecular mass of 28 kDa for TFIID under nondenaturing conditions [48].
  • An ELISA with the purified 28-kDa EBNA as antigen was used to quantitate anti-EBNA antibody in human serum samples [30].
  • Here we show that the activity of NF-AT in electrophoretic gel mobility-shift assays is greatly enhanced by a distinct protein with an apparent molecular mass of 28 kDa [49].
  • These biochemical results appeared entirely consistent with indirect immunofluorescence experiments, demonstrating that the 28-kDa protein resided within the perinuclear region of 37 degrees C cells in close proximity to the Golgi complex [50].
  • The affinity purified protein from both peaks was identical in Western blot analysis and had a molecular mass of 28 kDa under reducing conditions [51].


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  2. Specific reduction of calcium-binding protein (28-kilodalton calbindin-D) gene expression in aging and neurodegenerative diseases. Iacopino, A.M., Christakos, S. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  3. Sézary T-cell-activating factor induces functional interleukin 2 receptors on T-cells derived from patients with Sézary syndrome. Abrams, J.T., Ghosh, S.K., DeFreitas, E. Cancer Res. (1993) [Pubmed]
  4. Identification and characterization of human endometase (Matrix metalloproteinase-26) from endometrial tumor. Park, H.I., Ni, J., Gerkema, F.E., Liu, D., Belozerov, V.E., Sang, Q.X. J. Biol. Chem. (2000) [Pubmed]
  5. A 28-kDa protein from Mycobacterium leprae is a target of the human antibody response in lepromatous leprosy. Cherayil, B.J., Young, R.A. J. Immunol. (1988) [Pubmed]
  6. Erythropoietin protects dopaminergic neurons and improves neurobehavioral outcomes in juvenile rats after neonatal hypoxia-ischemia. Demers, E.J., McPherson, R.J., Juul, S.E. Pediatr. Res. (2005) [Pubmed]
  7. Differences in the social consequences of ethanol emerge during the course of adolescence in rats: social facilitation, social inhibition, and anxiolysis. Varlinskaya, E.I., Spear, L.P. Developmental psychobiology. (2006) [Pubmed]
  8. The calpain system. Goll, D.E., Thompson, V.F., Li, H., Wei, W., Cong, J. Physiol. Rev. (2003) [Pubmed]
  9. Placental transfer of inorganic ions and water. Stulc, J. Physiol. Rev. (1997) [Pubmed]
  10. Taspase1: a threonine aspartase required for cleavage of MLL and proper HOX gene expression. Hsieh, J.J., Cheng, E.H., Korsmeyer, S.J. Cell (2003) [Pubmed]
  11. Deletion of the serine 34 codon from the major peripheral myelin protein P0 gene in Charcot-Marie-Tooth disease type 1B. Kulkens, T., Bolhuis, P.A., Wolterman, R.A., Kemp, S., te Nijenhuis, S., Valentijn, L.J., Hensels, G.W., Jennekens, F.G., de Visser, M., Hoogendijk, J.E. Nat. Genet. (1993) [Pubmed]
  12. Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin. Allain, F.H., Bouvet, P., Dieckmann, T., Feigon, J. EMBO J. (2000) [Pubmed]
  13. Mechanism of action of Pseudomonas exotoxin. Identification of a rate-limiting step. Zdanovsky, A.G., Chiron, M., Pastan, I., FitzGerald, D.J. J. Biol. Chem. (1993) [Pubmed]
  14. The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein. Faucher, C., Capdevielle, J., Canal, I., Ferrara, P., Mazarguil, H., McGuire, W.L., Darbon, J.M. J. Biol. Chem. (1993) [Pubmed]
  15. Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein. Collin, M., Olsén, A. Mol. Microbiol. (2000) [Pubmed]
  16. Enzymatic Activities of Human Cytomegalovirus Maturational Protease Assemblin and Its Precursor (pPR, pUL80a): Maximal Activity of pPR Requires Self-Interaction through Its Scaffolding Domain. Brignole, E.J., Gibson, W. J. Virol. (2007) [Pubmed]
  17. Crystal structure of the 28 kDa glutathione S-transferase from Schistosoma haematobium. Johnson, K.A., Angelucci, F., Bellelli, A., Hervé, M., Fontaine, J., Tsernoglou, D., Capron, A., Trottein, F., Brunori, M. Biochemistry (2003) [Pubmed]
  18. cDNA characterization and chromosomal mapping of human golgi SNARE GS27 and GS28 to chromosome 17. Bui, T.D., Levy, E.R., Subramaniam, V.N., Lowe, S.L., Hong, W. Genomics (1999) [Pubmed]
  19. Cloning and expression of human deoxyguanosine kinase cDNA. Johansson, M., Karlsson, A. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  20. Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells. Marino, M.W., Pfeffer, L.M., Guidon, P.T., Donner, D.B. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  21. Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies. Banki, K., Maceda, J., Hurley, E., Ablonczy, E., Mattson, D.H., Szegedy, L., Hung, C., Perl, A. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  22. cDNA cloning of somatolactin, a pituitary protein related to growth hormone and prolactin. Ono, M., Takayama, Y., Rand-Weaver, M., Sakata, S., Yasunaga, T., Noso, T., Kawauchi, H. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  23. A v-SNARE implicated in intra-Golgi transport. Nagahama, M., Orci, L., Ravazzola, M., Amherdt, M., Lacomis, L., Tempst, P., Rothman, J.E., Söllner, T.H. J. Cell Biol. (1996) [Pubmed]
  24. The three-dimensional structure of human erythrocyte aquaporin CHIP. Walz, T., Smith, B.L., Agre, P., Engel, A. EMBO J. (1994) [Pubmed]
  25. A 21-kDa surface protein of Mycobacterium leprae binds peripheral nerve laminin-2 and mediates Schwann cell invasion. Shimoji, Y., Ng, V., Matsumura, K., Fischetti, V.A., Rambukkana, A. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  26. All-trans-retinoic acid induces CD52 expression in acute promyelocytic leukemia. Li, S.W., Tang, D., Ahrens, K.P., She, J.X., Braylan, R.C., Yang, L. Blood (2003) [Pubmed]
  27. Intron-encoded endonuclease I-TevI binds as a monomer to effect sequential cleavage via conformational changes in the td homing site. Mueller, J.E., Smith, D., Bryk, M., Belfort, M. EMBO J. (1995) [Pubmed]
  28. Purification and characterization of an inhibitor (soluble tumor necrosis factor receptor) for tumor necrosis factor and lymphotoxin obtained from the serum ultrafiltrates of human cancer patients. Gatanaga, T., Hwang, C.D., Kohr, W., Cappuccini, F., Lucci, J.A., Jeffes, E.W., Lentz, R., Tomich, J., Yamamoto, R.S., Granger, G.A. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  29. A model for the transcriptional regulation of the CYP2B1/B2 gene in rat liver. Prabhu, L., Upadhya, P., Ram, N., Nirodi, C.S., Sultana, S., Vatsala, P.G., Mani, S.A., Rangarajan, P.N., Surolia, A., Padmanaban, G. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  30. Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man. Milman, G., Scott, A.L., Cho, M.S., Hartman, S.C., Ades, D.K., Hayward, G.S., Ki, P.F., August, J.T., Hayward, S.D. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  31. Specific binding of chloroplast proteins in vitro to the 3' untranslated region of spinach chloroplast petD mRNA. Chen, H.C., Stern, D.B. Mol. Cell. Biol. (1991) [Pubmed]
  32. Crystal structure and kinetic analysis of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase. Lim, D., Park, H.U., De Castro, L., Kang, S.G., Lee, H.S., Jensen, S., Lee, K.J., Strynadka, N.C. Nat. Struct. Biol. (2001) [Pubmed]
  33. Identification of two messenger RNA cap binding proteins in wheat germ. Evidence that the 28-kDa subunit of eIF-4B and the 26-kDa subunit of eIF-4F are antigenically distinct polypeptides. Browning, K.S., Lax, S.R., Ravel, J.M. J. Biol. Chem. (1987) [Pubmed]
  34. Clusterin (complement lysis inhibitor) forms a high density lipoprotein complex with apolipoprotein A-I in human plasma. Jenne, D.E., Lowin, B., Peitsch, M.C., Böttcher, A., Schmitz, G., Tschopp, J. J. Biol. Chem. (1991) [Pubmed]
  35. Vitamin-D dependent 9 kDa calcium-binding protein gene: cDNA cloning, mRNA distribution and regulation. Thomasset, M., Desplan, C., Warembourg, M., Perret, C. Biochimie (1986) [Pubmed]
  36. Analysis of autoantibody repertoires in small- and medium-sized vessels vasculitides. Evidence for specific perturbations in polyarteritis nodosa, microscopic polyangiitis, Churg-Strauss syndrome and Wegener's granulomatosis. Chanseaud, Y., Tamby, M.C., Guilpain, P., Reinbolt, J., Kambouchner, M., Boyer, N., Noël, L.H., Guillevin, L., Boissier, M.C., Mouthon, L. J. Autoimmun. (2005) [Pubmed]
  37. Proteolytic mapping of human replication protein A: evidence for multiple structural domains and a conformational change upon interaction with single-stranded DNA. Gomes, X.V., Henricksen, L.A., Wold, M.S. Biochemistry (1996) [Pubmed]
  38. STAT1 phosphorylation and cleavage is regulated by the histamine (H4) receptor in human atopic and non-atopic lymphocytes. Horr, B., Borck, H., Thurmond, R., Grösch, S., Diel, F. Int. Immunopharmacol. (2006) [Pubmed]
  39. Impaired proteolytic processing of presenilin-1 in chromosome 14-linked familial Alzheimer's disease patient lymphocytes. Takahashi, H., Mercken, M., Honda, T., Saito, Y., Murayama, M., Song, S., Takashima, A. Neurosci. Lett. (1999) [Pubmed]
  40. Matrilysin cleavage of corneal collagen type XVIII NC1 domain and generation of a 28-kDa fragment. Lin, H.C., Chang, J.H., Jain, S., Gabison, E.E., Kure, T., Kato, T., Fukai, N., Azar, D.T. Invest. Ophthalmol. Vis. Sci. (2001) [Pubmed]
  41. The HLA proteins and a related protein of 28 kDa are preferentially induced by interferon-gamma in human WISH cells. Wallach, D. Eur. J. Immunol. (1983) [Pubmed]
  42. Characterization of the SCAN box encoding RAZ1 gene: analysis of cDNA transcripts, expression, and cellular localization. Sander, T.L., Morris, J.F. Gene (2002) [Pubmed]
  43. Localization of distinct functional domains on prekallikrein for interaction with both high molecular weight kininogen and activated factor XII in a 28-kDa fragment (amino acids 141-371). Page, J.D., Colman, R.W. J. Biol. Chem. (1991) [Pubmed]
  44. Mycobacterial antigen complex A60-specific T-cell repertoire during the course of pulmonary tuberculosis. Carlucci, S., Beschin, A., Tuosto, L., Ameglio, F., Gandolfo, G.M., Cocito, C., Fiorucci, F., Saltini, C., Piccolella, E. Infect. Immun. (1993) [Pubmed]
  45. N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex. Subramaniam, V.N., Loh, E., Hong, W. J. Biol. Chem. (1997) [Pubmed]
  46. Tumor protein D52 (TPD52): a novel B-cell/plasma-cell molecule with unique expression pattern and Ca(2+)-dependent association with annexin VI. Tiacci, E., Orvietani, P.L., Bigerna, B., Pucciarini, A., Corthals, G.L., Pettirossi, V., Martelli, M.P., Liso, A., Benedetti, R., Pacini, R., Bolli, N., Pileri, S., Pulford, K., Gambacorta, M., Carbone, A., Pasquarello, C., Scherl, A., Robertson, H., Sciurpi, M.T., Alunni-Bistocchi, G., Binaglia, L., Byrne, J.A., Falini, B. Blood (2005) [Pubmed]
  47. Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor. Mowla, S.J., Farhadi, H.F., Pareek, S., Atwal, J.K., Morris, S.J., Seidah, N.G., Murphy, R.A. J. Biol. Chem. (2001) [Pubmed]
  48. Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity. Horikoshi, M., Wang, C.K., Fujii, H., Cromlish, J.A., Weil, P.A., Roeder, R.G. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  49. Characterization of a protein that regulates the DNA-binding activity of NF-AT, the nuclear factor of activated T cells. Granelli-Piperno, A., McHugh, P. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  50. Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein. Arrigo, A.P., Suhan, J.P., Welch, W.J. Mol. Cell. Biol. (1988) [Pubmed]
  51. Leukophysin: a 28-kDa granule membrane protein of leukocytes. Abdelhaleem, M.M., Hatskelzon, L., Dalal, B.I., Gerrard, J.M., Greenberg, A.H. J. Immunol. (1991) [Pubmed]
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