Disease relevance of Dpt

• In conclusion, dermatopontin may be involved in the pathogenesis and growth of the prostate cancer [1].
• AIMS: To examine Escherichia coli strains EQ1, DH5alpha, BLR and BL21 for known pathogenic mechanisms [2].

High impact information on Dpt

• The full-length EQ-1 cDNA, cloned from a mouse lung cDNA library, is 1673 bp in length and consists of 26 bp 5' untranslated region, 603 bp coding region, and 1044 bp 3' untranslated region, the latter of which harbors two polyadenylation signals [3].
• The stably transfected cell line harboring EQ-1 driven by an inducible promoter showed approximately 50% inhibition on cell proliferation after being treated with an inducer for 5 days [3].
• This study was conducted to determine whether DPT has a direct role in corneal matrix organization by investigating the ultrastructure of Dpt-null (Dpt(-/-)) mouse corneas [4].
• RESULTS: Light microscopy demonstrated that Dpt(-/-) corneas in 2-month-old mice showed a 24% reduction in average stromal thickness compared with wild type (P < 0.001) [4].
• PURPOSE: Dermatopontin (DPT) is an abundant component of the stromal extracellular matrix; however, its function in the cornea is poorly understood [4].

Biological context of Dpt

• This high molecular weight, multi-reactive antigen corresponded to allergen Dpt 4, suggesting that Dpt 4 exists as isoallergens, each differing in the degree of glycosylation or in the type of carbohydrate moiety comprising the glycan part of the allergen [5].
• Using the PCR-select cDNA subtraction method, dermatopontin was identified in the uterus of a pregnant mouse on day 4 of gestation [6].
• In silico analysis revealed progesterone receptor binding sites in the dermatopontin promoter region [6].

Anatomical context of Dpt

• Remarkably, light microscopy study showed a significant decrease in the relative thickness of the dermis in dermatopontin-null mice compared with wild-type mice (45.2 +/- 3.09% and 57.8 +/- 4.25%, respectively) [7].
• Using a chick model, strains EQ1, BLR and BL21 invaded livers but not spleens; only strain EQ1 persisted in the chick gut [2].
• Spatio-temporal expression and regulation of dermatopontin in the early pregnant mouse uterus [6].

Associations of Dpt with chemical compounds

• Dermatopontin is a tyrosine-rich acidic extracellular matrix protein of 22 kDa with possible functions in cell-matrix interactions and matrix assembly [1].
• BMP Stimulation of Alkaline Phosphatase Activity in Pluripotent Mouse C2C12 Cells is Inhibited by Dermatopontin, One of the Most Abundant Low Molecular Weight Proteins in Demineralized Bone Matrix [8].
• They also suggest that, at the time of implantation, dermatopontin expression is primarily regulated spatio-temporally by progesterone via progesterone receptors, and is modulated by the decidual response during implantation [6].
• Collectively, these results strongly suggest that dermatopontin expression is steroid-dependent [6].
• Progesterone markedly induced dermatopontin expression in ovariectomized uteri within 4 h of administration, whereas estrogen had little effect [6].

Regulatory relationships of Dpt

• Additionally, we have established mouse dermatopontin transgenic mice under the control of the rat probasin promoter [1].

Analytical, diagnostic and therapeutic context of Dpt

• Examination of Dpt(-/-) stroma by transmission electron microscopy indicated significant disruption of fibril spacing within the posterior lamellae, whereas the mid and anterior regions appeared largely unaffected compared with wild type [4].
• Computer-assisted peptide fingerprint analysis of the MS profiles was used to identify C-terminal lysine-6-oxidase; dermatopontin (DPT); histones H2A2, H2A3, and H2B; and trace amounts of gamma-actin [8].

References