The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Pbsn  -  probasin

Mus musculus

Synonyms: PB, Prbs, Probasin
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Pbsn


High impact information on Pbsn

  • Use of the probasin promoter ARR2PB to express Bax in androgen receptor-positive prostate cancer cells [3].
  • Therefore, we developed an adenoviral construct (Av-ARR2PB-Bax) in which the probasin promoter, modified to contain two androgen response elements, drives Bax expression [3].
  • However, association was not detected with mutant receptors inactivated by an internal deletion or a point mutation, Trp282 to Arg, in a membrane-proximal cytoplasmic region (PB or PM4 mutant, respectively) [4].
  • A luciferase reporter gene was directed to the prostate epithelium using three composite promoters called human kallikrein 2 (hK2)-E3/P, PSA-E2/P, and ARR2PB, derived from hK2, PSA, and rat probasin regulatory elements, to generate the EZC1, EZC2, and EZC3-prostate mice, respectively [5].
  • We generated four independent lines of transgenic mice with targeted overexpression of FGF8b in the prostatic epithelium using an improved rat probasin promoter, ARR(2)PB [6].

Chemical compound and disease context of Pbsn


Biological context of Pbsn


Anatomical context of Pbsn

  • The expression of probasin was primarily localized to the apical membrane of differentiated secretory epithelium [1].
  • Consistent with this finding, we observed that the SV40 Tag and endogenous mouse probasin genes are expressed at low levels in the thymus [15].
  • A second-generation adenoviral vector was constructed with the modified prostate-specific probasin promoter, ARR2PB, directing expression of an HA-tagged Bax gene and a green fluorescent protein reporter translated from an internal ribosome entry site (ARR2PB.Bax.GFP) [16].
  • Thus, a composite probasin promoter (ARR2PB) coupled to the bacterial chloramphenicol acetyltransferase reporter (ARR2PBCAT) was generated and tested in prostatic and nonprostatic cell lines and in a transgenic mouse model [13].
  • (3) PB-activated macrophages are more sensitive to TrB blockage [17].

Associations of Pbsn with chemical compounds

  • Probasin is a prostate-specific gene originally isolated from the rat and has been exploited as a marker of prostate differentiation and to elucidate androgen action [1].
  • The addition of low doses of the cationic polypeptide antibiotic, polymyxin B (PB), to cultures of mouse spleen cells inhibits lipopolysaccharide-(LPS) induced DNA synthesis but not that stimulated by PPD, PHA, or Con A [18].
  • Selective activation of the probasin androgen-responsive region by steroid hormones [19].
  • Surprisingly, PB also reduced proliferation to detoxified LPS [20].
  • The tumor-suppressive effect of PB disappeared after macrophage system blockage with TrB [17].

Regulatory relationships of Pbsn


Other interactions of Pbsn


Analytical, diagnostic and therapeutic context of Pbsn


  1. Isolation and characterization of mouse probasin: An androgen-regulated protein specifically expressed in the differentiated prostate. Johnson, M.A., Hernandez, I., Wei, Y., Greenberg, N. Prostate (2000) [Pubmed]
  2. A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential. Masumori, N., Thomas, T.Z., Chaurand, P., Case, T., Paul, M., Kasper, S., Caprioli, R.M., Tsukamoto, T., Shappell, S.B., Matusik, R.J. Cancer Res. (2001) [Pubmed]
  3. Use of the probasin promoter ARR2PB to express Bax in androgen receptor-positive prostate cancer cells. Andriani, F., Nan, B., Yu, J., Li, X., Weigel, N.L., McPhaul, M.J., Kasper, S., Kagawa, S., Fang, B., Matusik, R.J., Denner, L., Marcelli, M. J. Natl. Cancer Inst. (2001) [Pubmed]
  4. Erythropoietin induces association of the JAK2 protein tyrosine kinase with the erythropoietin receptor in vivo. Miura, O., Nakamura, N., Quelle, F.W., Witthuhn, B.A., Ihle, J.N., Aoki, N. Blood (1994) [Pubmed]
  5. EZC-prostate models offer high sensitivity and specificity for noninvasive imaging of prostate cancer progression and androgen receptor action. Seethammagari, M.R., Xie, X., Greenberg, N.M., Spencer, D.M. Cancer Res. (2006) [Pubmed]
  6. Fibroblast growth factor 8 isoform B overexpression in prostate epithelium: a new mouse model for prostatic intraepithelial neoplasia. Song, Z., Wu, X., Powell, W.C., Cardiff, R.D., Cohen, M.B., Tin, R.T., Matusik, R.J., Miller, G.J., Roy-Burman, P. Cancer Res. (2002) [Pubmed]
  7. Loss of androgen receptor transcriptional activity at the G(1)/S transition. Martinez, E.D., Danielsen, M. J. Biol. Chem. (2002) [Pubmed]
  8. Monogene and polygene therapy for the treatment of experimental prostate cancers by use of apoptotic genes bax and bad driven by the prostate-specific promoter ARR(2)PB. Zhang, Y., Yu, J., Unni, E., Shao, T.C., Nan, B., Snabboon, T., Kasper, S., Andriani, F., Denner, L., Marcelli, M. Hum. Gene Ther. (2002) [Pubmed]
  9. Role of cadmium in the regulation of AR gene expression and activity. Martin, M.B., Voeller, H.J., Gelmann, E.P., Lu, J., Stoica, E.G., Hebert, E.J., Reiter, R., Singh, B., Danielsen, M., Pentecost, E., Stoica, A. Endocrinology (2002) [Pubmed]
  10. Effect of polymyxin B on intestinal bacterial translocation in Pseudomonas aeruginosa wound-colonized burned mice. Dijkstra, H.M., Manson, W.L., Klasen, H.J., van der Waaij, D. European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes. (1992) [Pubmed]
  11. Chemoprevention of prostate carcinogenesis by alpha-difluoromethylornithine in TRAMP mice. Gupta, S., Ahmad, N., Marengo, S.R., MacLennan, G.T., Greenberg, N.M., Mukhtar, H. Cancer Res. (2000) [Pubmed]
  12. The impact of bcl-2 expression and bax deficiency on prostate homeostasis in vivo. Bruckheimer, E.M., Cho, S., Brisbay, S., Johnson, D.J., Gingrich, J.R., Greenberg, N., McDonnell, T.J. Oncogene (2000) [Pubmed]
  13. A small composite probasin promoter confers high levels of prostate-specific gene expression through regulation by androgens and glucocorticoids in vitro and in vivo. Zhang, J., Thomas, T.Z., Kasper, S., Matusik, R.J. Endocrinology (2000) [Pubmed]
  14. Gene expression analysis of prostate hyperplasia in mice overexpressing the prolactin gene specifically in the prostate. Dillner, K., Kindblom, J., Flores-Morales, A., Shao, R., Törnell, J., Norstedt, G., Wennbo, H. Endocrinology (2003) [Pubmed]
  15. Clonal deletion of simian virus 40 large T antigen-specific T cells in the transgenic adenocarcinoma of mouse prostate mice: an important role for clonal deletion in shaping the repertoire of T cells specific for antigens overexpressed in solid tumors. Zheng, X., Gao, J.X., Zhang, H., Geiger, T.L., Liu, Y., Zheng, P. J. Immunol. (2002) [Pubmed]
  16. Prostate-specific expression of Bax delivered by an adenoviral vector induces apoptosis in LNCaP prostate cancer cells. Lowe, S.L., Rubinchik, S., Honda, T., McDonnell, T.J., Dong, J.Y., Norris, J.S. Gene Ther. (2001) [Pubmed]
  17. The effect of blockage of macrophage on tumor-suppressive activity of polyactin B. Xu, C., Wang, X., Wang, B., Han, S., Zhang, Y., Si, L. Chin. Med. J. (1998) [Pubmed]
  18. Inhibition of the mitogenic response to lipopolysaccharide (LPS) in mouse spleen cells by polymyxin B. Jacobs, D.M., Morrison, D.C. J. Immunol. (1977) [Pubmed]
  19. Selective activation of the probasin androgen-responsive region by steroid hormones. Kasper, S., Rennie, P.S., Bruchovsky, N., Lin, L., Cheng, H., Snoek, R., Dahlman-Wright, K., Gustafsson, J.A., Shiu, R.P., Sheppard, P.C., Matusik, R.J. J. Mol. Endocrinol. (1999) [Pubmed]
  20. Proliferative and T-cell specific interleukin (IL-2/IL-4) production responses in spleen cells from mice vaccinated with aroA live attenuated Salmonella vaccines. Villarreal, B., Mastroeni, P., de Hormaeche, R.D., Hormaeche, C.E. Microb. Pathog. (1992) [Pubmed]
  21. Engineered IGF-I expression induces glandular enlargement in the murine prostate. Konno-Takahashi, N., Takeuchi, T., Shimizu, T., Nishimatsu, H., Fukuhara, H., Kamijo, T., Moriyama, N., Tejima, S., Kitamura, T. J. Endocrinol. (2003) [Pubmed]
  22. Extracellular matrix dermatopontin modulates prostate cell growth in vivo. Takeuchi, T., Suzuki, M., Kumagai, J., Kamijo, T., Sakai, M., Kitamura, T. J. Endocrinol. (2006) [Pubmed]
  23. Prostatic intraepithelial neoplasia and adenocarcinoma in mice expressing a probasin-Neu oncogenic transgene. Li, Z., Szabolcs, M., Terwilliger, J.D., Efstratiadis, A. Carcinogenesis (2006) [Pubmed]
  24. Epithelial defect in prostates of Stat5a-null mice. Nevalainen, M.T., Ahonen, T.J., Yamashita, H., Chandrashekar, V., Bartke, A., Grimley, P.M., Robinson, G.W., Hennighausen, L., Rui, H. Lab. Invest. (2000) [Pubmed]
  25. Effects of aryl hydrocarbon receptor null mutation and in utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on prostate and seminal vesicle development in C57BL/6 mice. Lin, T.M., Ko, K., Moore, R.W., Simanainen, U., Oberley, T.D., Peterson, R.E. Toxicol. Sci. (2002) [Pubmed]
  26. Prostate cancer in a transgenic mouse. Greenberg, N.M., DeMayo, F., Finegold, M.J., Medina, D., Tilley, W.D., Aspinall, J.O., Cunha, G.R., Donjacour, A.A., Matusik, R.J., Rosen, J.M. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  27. Prostate hyperplasia in a transgenic mouse with prostate-specific expression of prolactin. Kindblom, J., Dillner, K., Sahlin, L., Robertson, F., Ormandy, C., Törnell, J., Wennbo, H. Endocrinology (2003) [Pubmed]
  28. An allograft model of androgen independent prostatic neuroendocrine carcinoma derived from a large probasin promoter-T antigen transgenic mouse line. Masumori, N., Tsuchiya, K., Tu, W.H., Lee, C., Kasper, S., Tsukamoto, T., Shappell, S.B., Matusik, R.J. J. Urol. (2004) [Pubmed]
WikiGenes - Universities