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Gene Review

cyp19a1b  -  cytochrome P450, family 19, subfamily A,...

Danio rerio

Synonyms: AroB, CYP19a2, P450aromB, SO:0000704, cyp19, ...
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High impact information on cyp19a1b


Biological context of cyp19a1b


Anatomical context of cyp19a1b

  • When microinjected into fertilized zebrafish eggs, a cyp19b promoter-driven green fluorescent protein (GFP) reporter (but not cyp19a) is expressed in neurons of 30-48 hpf embryos, most prominently in retinal ganglion cells (RGCs) and their projections to optic tectum [4].
  • In vivo data in zebrafish have shown that E2 up-regulates AroB only in radial glial cells [7].
  • Comparative distribution of AroB and estrogen receptor (ERalpha, ERbeta1, and ERbeta2) expression indicates that the preoptic region and hypothalamus are major target for locally produced estradiol (E2) which is likely involved in controlling expression of genes implicated in neuroendocrine regulations [7].
  • The expression of AroB in radial cells, well known for their roles in neurogenesis and now considered as progenitor cells, suggests that local E2 production within these cells could influence the well-documented capacity of the brain of teleosts to grow during adulthood [7].
  • When compared to whole ovaries, unfertilized eggs had similar levels of P450aromA but enrichment of P450aromB, which suggests preferential synthesis or accumulation in mature oocytes [8].

Other interactions of cyp19a1b

  • As measured by increased P450aromB mRNA, a functional estrogen response system is first detected 24-48 h post-fertilization (hpf), consistent with the onset of estrogen receptor (ER) expression (alpha, beta, and gamma) [4].

Analytical, diagnostic and therapeutic context of cyp19a1b

  • As a first step toward understanding estrogen's role in neurodevelopment, a PCR cloning strategy was used to isolate complementary DNAs encoding two distinct cytochrome P450 aromatase isoforms in adult zebrafish (Danio rerio) brain and ovary (termed P450aromB and P450aromA, respectively) [2].
  • On Northern blots, a single 4.4-kb transcript of the P450aromB subtype was identified in brain, and a 2.1-kb transcript of the P450aromA subtype in ovary, but RT-PCR showed a degree of overlapping expression [2].
  • RT-PCR/Southern transfer analysis showed that E(2) (0.01-10 microM) upregulated P450aromB in a dose-response manner [9].


  1. Two Cyp19 (P450 aromatase) genes on duplicated zebrafish chromosomes are expressed in ovary or brain. Chiang, E.F., Yan, Y.L., Guiguen, Y., Postlethwait, J., Chung Bc, n.u.l.l. Mol. Biol. Evol. (2001) [Pubmed]
  2. Distinct cytochrome P450 aromatase isoforms in zebrafish (Danio rerio) brain and ovary are differentially programmed and estrogen regulated during early development. Kishida, M., Callard, G.V. Endocrinology (2001) [Pubmed]
  3. Expression and estrogen-dependent regulation of the zebrafish brain aromatase gene. Menuet, A., Pellegrini, E., Brion, F., Gueguen, M.M., Anglade, I., Pakdel, F., Kah, O. J. Comp. Neurol. (2005) [Pubmed]
  4. Differential tissue distribution, developmental programming, estrogen regulation and promoter characteristics of cyp19 genes in teleost fish. Callard, G.V., Tchoudakova, A.V., Kishida, M., Wood, E. J. Steroid Biochem. Mol. Biol. (2001) [Pubmed]
  5. Phylogeny, expression and enzyme activity of zebrafish cyp19 (P450 aromatase) genes. Tong, S.K., Chiang, E.F., Hsiao, P.H., Chung, B. J. Steroid Biochem. Mol. Biol. (2001) [Pubmed]
  6. Promoter characteristics of two cyp19 genes differentially expressed in the brain and ovary of teleost fish. Tchoudakova, A., Kishida, M., Wood, E., Callard, G.V. J. Steroid Biochem. Mol. Biol. (2001) [Pubmed]
  7. Relationships between aromatase and estrogen receptors in the brain of teleost fish. Pellegrini, E., Menuet, A., Lethimonier, C., Adrio, F., Gueguen, M.M., Tascon, C., Anglade, I., Pakdel, F., Kah, O. Gen. Comp. Endocrinol. (2005) [Pubmed]
  8. Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation. Sawyer, S.J., Gerstner, K.A., Callard, G.V. Gen. Comp. Endocrinol. (2006) [Pubmed]
  9. Estrogen and xenoestrogens upregulate the brain aromatase isoform (P450aromB) and perturb markers of early development in zebrafish (Danio rerio). Kishida, M., McLellan, M., Miranda, J.A., Callard, G.V. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (2001) [Pubmed]
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