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Gene Review

ZNF185  -  zinc finger protein 185 (LIM domain)

Homo sapiens

Synonyms: LIM domain protein ZNF185, P1-A, Zinc finger protein 185
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Disease relevance of ZNF185


High impact information on ZNF185

  • To explore their potential relevance in lung tumours, levels of CYP3A4 and ZNF185 transcripts were evaluated in seven additional lung cancer cell lines [1].
  • We chose the antigen encoded by P1A, a well-characterized murine cancer germ line gene [3].
  • Because the CreER gene itself is also flanked by loxP sites, the activation of CreER also induces the deletion of its own coding sequence and thereby allows melanocyte-specific expression of genes H-ras and P1A, which are located downstream on the same transgene [3].
  • Thus, transcriptional silencing of ZNF185 by methylation in prostate tumor tissues implicates the ZNF185 gene in prostate tumorigenesis [2].
  • In a liver cell line, the P1 promoter appeared to be the strongest; in a lung cell line, the P1A promoter [6].

Chemical compound and disease context of ZNF185

  • Polyacrylamide gels electrophoresis and immunoenzymatic assay with specific antibodies to group A rotavirus, serotypes G1-4, P1A, and P1B were carried out on all the samples [7].

Biological context of ZNF185

  • We extended these observations to 27 lung primary tumours using real-time RT-PCR (TaqMan) and observed that four (15%) and 14 (52%) of them had BRG1 and ZNF185 downregulation, respectively, when compared with normal lung [1].
  • Moreover, methylation-specific PCR confirmed methylation of the 5'CpG islands of the ZNF185 gene in all of the metastatic tissues and 44% of the localized tumor tissues, as well as in the prostate cancer cell lines tested [2].
  • Since LIM proteins regulate cellular proliferation and/or differentiation by diverse mechanisms, and some have directly been associated with disease, conceivably ZNF185 may represent a candidate for a disease-causing gene linked to Xq28 [8].
  • The P1A gene, which encodes the P815A antigen, was modified by the addition of a short sequence coding for a tag epitope recognized by the monoclonal antibody AU1, and cloned into the eukaryotic expression vector pBKCMV, resulting in plasmid pBKCMV-P1A [9].
  • Transfection experiments in HepG2 cells revealed that fur P1 promoter is the strongest and the most sensitive to TGFbeta1 stimulation (5 ng/ml) (3.2-fold vs. 2.4-fold for P1A and 2.1-fold for P1B) [10].

Anatomical context of ZNF185

  • We show that ZNF185 is a novel actin-cytoskeleton-associated Lin-l 1, Isl-1 and Mec-3 (LIM) domain-containing protein that localizes to F-actin structures, and is enriched at focal adhesions [11].
  • The P1A gene, which encodes for the P815A antigen, is silent in most normal tissues with the exception of testis and placenta [12].
  • We identified labyrinthine trophoblasts as the placental cells expressing P1A [13].
  • Tumor peptides derived from antigens P198 or P1A were targeted to antigen-presenting cells (APC) by ex vivo pulsing [14].
  • By immunohistochemistry with a rabbit antiserum directed against the P1A protein, we identified spermatogonia as the testicular cells expressing P1A [13].

Associations of ZNF185 with chemical compounds

  • The P1G and P1A mutants showed 10- and 4-fold decreases, respectively, in catalysis of exchange of the C3 proton of the substrate 2-oxo-1,6-hexanedioate, consistent with the lower basicities of Gly-1 and Ala-1 compared to Pro-1 [15].
  • Mechanism of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor: properties of the P1G, P1A, Y95F, and N97A mutants [16].
  • The P1A alloantigen system is a sensitive indicator of the structural integrity of the amino-terminal domain of the human integrin beta 3 subunit [17].

Analytical, diagnostic and therapeutic context of ZNF185

  • A single intramuscular injection of 100 microg of pBKCMV-P1A induced the expression of P1A mRNA for at least 4 months [9].
  • The results obtained by these genetic techniques were evaluated against those obtained by an enzyme immunoassay (EIA) incorporating neutralizing monoclonal antibodies (N-MAbs) reacting with three major human P serotypes (serotypes P1A, P1B, and P2A) [18].
  • A prominent sequence-dependent DNA-protein complex (C-I) was detected in electrophoretic mobility shift assays with P1-A using RT4-D6P2T nuclear extract and was localized to a minimal 21 bp region within P1-A [19].
  • DNA-based vaccination against tumors expressing the P1A antigen [20].


  1. Transcriptional targets of the chromatin-remodelling factor SMARCA4/BRG1 in lung cancer cells. Medina, P.P., Carretero, J., Ballestar, E., Angulo, B., Lopez-Rios, F., Esteller, M., Sanchez-Cespedes, M. Hum. Mol. Genet. (2005) [Pubmed]
  2. Transcriptional silencing of zinc finger protein 185 identified by expression profiling is associated with prostate cancer progression. Vanaja, D.K., Cheville, J.C., Iturria, S.J., Young, C.Y. Cancer Res. (2003) [Pubmed]
  3. An inducible mouse model of melanoma expressing a defined tumor antigen. Huijbers, I.J., Krimpenfort, P., Chomez, P., van der Valk, M.A., Song, J.Y., Inderberg-Suso, E.M., Schmitt-Verhulst, A.M., Berns, A., Van den Eynde, B.J. Cancer Res. (2006) [Pubmed]
  4. Distribution of conserved and specific epitopes on the VP8 subunit of rotavirus VP4. Larralde, G., Gorziglia, M. J. Virol. (1992) [Pubmed]
  5. Lipoprotein(a) and the significance of the association between platelet glycoprotein IIIa polymorphisms and the risk of premature myocardial infarction. Joven, J., Simó, J.M., Vilella, E., Camps, J., Masana, L., de Febrer, G., Camprubí, M., Richart, C., Bardaji, A., Casao, E., Pocovi, M., Civeira, F. Atherosclerosis (1998) [Pubmed]
  6. Expression of the dibasic proprotein processing enzyme furin is directed by multiple promoters. Ayoubi, T.A., Creemers, J.W., Roebroek, A.J., Van de Ven, W.J. J. Biol. Chem. (1994) [Pubmed]
  7. Acute gastroenteritis associated with rotavirus in adults. del Refugio González-Losa, M., Polanco-Marín, G.G., Manzano-Cabrera, L., Puerto-Solís, M. Arch. Med. Res. (2001) [Pubmed]
  8. Genomic structure of a novel LIM domain gene (ZNF185) in Xq28 and comparisons with the orthologous murine transcript. Heiss, N.S., Gloeckner, G., Bächner, D., Kioschis, P., Klauck, S.M., Hinzmann, B., Rosenthal, A., Herman, G.E., Poustka, A. Genomics (1997) [Pubmed]
  9. CTL response and protection against P815 tumor challenge in mice immunized with DNA expressing the tumor-specific antigen P815A. Rosato, A., Zambon, A., Milan, G., Ciminale, V., D'Agostino, D.M., Macino, B., Zanovello, P., Collavo, D. Hum. Gene Ther. (1997) [Pubmed]
  10. Involvement of Smads in TGFbeta1-induced furin (fur) transcription. Blanchette, F., Rudd, P., Grondin, F., Attisano, L., Dubois, C.M. J. Cell. Physiol. (2001) [Pubmed]
  11. ZNF185, an actin-cytoskeleton-associated growth inhibitory LIM protein in prostate cancer. Zhang, J.S., Gong, A., Young, C.Y. Oncogene (2007) [Pubmed]
  12. Induction of P815 tumor immunity by recombinant Semliki Forest virus expressing the P1A gene. Colmenero, P., Liljeström, P., Jondal, M. Gene Ther. (1999) [Pubmed]
  13. The expression of mouse gene P1A in testis does not prevent safe induction of cytolytic T cells against a P1A-encoded tumor antigen. Uyttenhove, C., Godfraind, C., Lethé, B., Amar-Costesec, A., Renauld, J.C., Gajewski, T.F., Duffour, M.T., Warnier, G., Boon, T., Van den Eynde, B.J. Int. J. Cancer (1997) [Pubmed]
  14. Improved efficacy of dendritic cell vaccines and successful immunization with tumor antigen peptide-pulsed peripheral blood mononuclear cells by coadministration of recombinant murine interleukin-12. Fallarino, F., Uyttenhove, C., Boon, T., Gajewski, T.F. Int. J. Cancer (1999) [Pubmed]
  15. Kinetic and structural effects of mutations of the catalytic amino-terminal proline in 4-oxalocrotonate tautomerase. Czerwinski, R.M., Johnson, W.H., Whitman, C.P. Biochemistry (1997) [Pubmed]
  16. Mechanism of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor: properties of the P1G, P1A, Y95F, and N97A mutants. Stamps, S.L., Taylor, A.B., Wang, S.C., Hackert, M.L., Whitman, C.P. Biochemistry (2000) [Pubmed]
  17. The P1A alloantigen system is a sensitive indicator of the structural integrity of the amino-terminal domain of the human integrin beta 3 subunit. Kunicki, T.J., Honda, S., Dawson, B., Honda, Y., Ruan, C., Aster, R.H. Blood Cells Mol. Dis. (1995) [Pubmed]
  18. Comparison of enzyme immunoassay, PCR, and type-specific cDNA probe techniques for identification of group A rotavirus gene 4 types (P types). Masendycz, P.J., Palombo, E.A., Gorrell, R.J., Bishop, R.F. J. Clin. Microbiol. (1997) [Pubmed]
  19. Identification of a positive regulatory element in the myelin-specific promoter of the PMP22 gene. Hai, M., Bidichandani, S.I., Patel, P.I. J. Neurosci. Res. (2001) [Pubmed]
  20. DNA-based vaccination against tumors expressing the P1A antigen. Rosato, A., Milan, G., Collavo, D., Zanovello, P. Methods (1999) [Pubmed]
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