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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Genetic Techniques

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Disease relevance of Genetic Techniques


High impact information on Genetic Techniques

  • We have used a combination of histological, molecular, and genetic techniques to investigate the flightless Drosophila mutant raised [6].
  • I set out to integrate, wherever possible, pathological changes in tissues and cells as determined by light, scanning, and TEM with chemical, biochemical, molecular, biological, and genetic techniques [7].
  • The molecular basis for the marked difference between primate and rodent cells in sensitivity to the cardiac glycoside ouabain has been established by genetic techniques [8].
  • Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein [9].
  • The segmental flexibility and complement fixation activity were measured of six genetically engineered molecules (the four human IgG isotypes, mouse IgG3 and rabbit IgG) and the remaining three mouse IgG isotypes, (IgG1, IgG2a and IgG2b), isolated previously by somatic cell genetic techniques [10].

Biological context of Genetic Techniques


Anatomical context of Genetic Techniques

  • We employ two complementary genetic techniques to identify genes that regulate the default IL-4 secretion profiles of T cells from BALB/c and B6 mice [16].

Associations of Genetic Techniques with chemical compounds


Gene context of Genetic Techniques

  • Tester strains were subsequently used for the reintroduction of various combinations of wild-type and mutated RAS2 and CDC25 genes by genetic techniques, as well as for in vitro reconstitution assays with the corresponding proteins [22].
  • The powerful genetic techniques offered by these organisms has allowed new components of the EGFR signal transduction pathway to be identified as well as lending insight into the basis of tissue specificity of signaling [23].
  • We have designed genetic techniques that select directly for unc-54 mutants [24].
  • Translocations occurred in the RAD52+ genetic background and were characterized by orthogonal field alternating gel electrophoresis of yeast chromosomal DNA and by standard genetic techniques [25].
  • Toward this end, we have used both biochemical and genetic techniques to probe potential oligomerization interactions of P-gp [26].

Analytical, diagnostic and therapeutic context of Genetic Techniques

  • The results obtained by these genetic techniques were evaluated against those obtained by an enzyme immunoassay (EIA) incorporating neutralizing monoclonal antibodies (N-MAbs) reacting with three major human P serotypes (serotypes P1A, P1B, and P2A) [27].


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  10. Segmental flexibility and complement fixation of genetically engineered chimeric human, rabbit and mouse antibodies. Dangl, J.L., Wensel, T.G., Morrison, S.L., Stryer, L., Herzenberg, L.A., Oi, V.T. EMBO J. (1988) [Pubmed]
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  16. Linkage analysis of the genetic determinants of T-cell IL-4 secretion, and identification of Flj20274 as a putative candidate gene. Choi, P., Xanthaki, D., Rose, S.J., Haywood, M., Reiser, H., Morley, B.J. Genes Immun. (2005) [Pubmed]
  17. Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Conklin, P.L., Norris, S.R., Wheeler, G.L., Williams, E.H., Smirnoff, N., Last, R.L. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  18. Drug targets: turning the channel (on) for sedation. Sandstrom, D.J., Nash, H. Curr. Biol. (2004) [Pubmed]
  19. Lipopolysaccharide core phosphates are required for viability and intrinsic drug resistance in Pseudomonas aeruginosa. Walsh, A.G., Matewish, M.J., Burrows, L.L., Monteiro, M.A., Perry, M.B., Lam, J.S. Mol. Microbiol. (2000) [Pubmed]
  20. Zinc absorption from a low-phytic acid maize. Adams, C.L., Hambidge, M., Raboy, V., Dorsch, J.A., Sian, L., Westcott, J.L., Krebs, N.F. Am. J. Clin. Nutr. (2002) [Pubmed]
  21. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Parker, B., Marinus, M.G. Gene (1988) [Pubmed]
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  23. Regulation of EGF receptor signaling in the fruitfly D. melanogaster and the nematode C. elegans. Lacenere, C.J., Sternberg, P.W. Breast disease. (2000) [Pubmed]
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  27. Comparison of enzyme immunoassay, PCR, and type-specific cDNA probe techniques for identification of group A rotavirus gene 4 types (P types). Masendycz, P.J., Palombo, E.A., Gorrell, R.J., Bishop, R.F. J. Clin. Microbiol. (1997) [Pubmed]
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