The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

DAP3  -  death associated protein 3

Homo sapiens

Synonyms: 28S ribosomal protein S29, mitochondrial, DAP-3, DKFZp686G12159, Death-associated protein 3, Ionizing radiation resistance conferring protein, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of DAP3


High impact information on DAP3

  • Interaction of DAP3 and FADD only after cellular disruption [3].
  • We have found that the GTP-binding protein DAP3 binds directly (with high affinity) to the death domain of TNF-related apoptosis-inducing ligand (TRAIL) receptors, and is required for TRAIL-induced apoptosis [4].
  • Expression of anti-sense DAP3 RNA and of the dominant interfering form of DAP3 both protected cells from apoptosis induced by activation of Fas and tumor necrosis factor alpha (TNF-alpha) receptors [5].
  • We also showed that DAP3 is phosphorylated by kinase Akt (PKB), and active Akt can nullify apoptosis induction by DAP3 [6].
  • Conversely, overexpression of DAP3 augmented cell death and caspase activation induced by cell detachment [6].

Biological context of DAP3

  • TRAIL-induced apoptosis is independent of the mitochondrial apoptosis mediator DAP3 [7].
  • Involvement of DAP3 in anoikis signaling demonstrates a novel role for this GTP-binding protein in apoptosis induction caused by cell detachment [6].
  • Mutation of a consensus Akt phosphorylation site in DAP3 renders it resistant to suppression by active Akt in cells [6].
  • Down-regulation of DAP3 expression by antisense oligonucleotides inhibited anoikis [6].
  • Although the levels of pro-Caspase-8 appeared sufficient, inadequate levels of Fas-associated death domain protein (FADD) and DAP3 may preclude assembly of the death-inducing signaling complex [8].

Anatomical context of DAP3

  • To address these discrepancies, the intracellular localization of DAP3 after apoptosis induction in human T-lymphocytes with recombinant TRAIL was analyzed [7].
  • TRAIL-induced and DR4-mediated apoptosis in Jurkat cells is independent of DAP3 [7].
  • Recent reports have suggested that DAP3 is localized to the mitochondria, but no functional significance of this localization has been reported so far [9].
  • Ectopic expression of DAP3 in HeLa cells was not compatible with cell growth, resulting in a 16-fold reduction in the number of drug-resistant stable clones [10].
  • The Death Associated Protein 3 (DAP3), a GTP-binding constituent of the small subunit of the mitochondrial ribosome, is implicated in the TNFalpha and IFNgamma apoptotic pathways of the cell and is involved in the maintenance of the mitochondrial network [11].

Associations of DAP3 with chemical compounds


Physical interactions of DAP3

  • In vitro experiments showed that the full-length DAP3 also interacted with GR [12].

Other interactions of DAP3


Analytical, diagnostic and therapeutic context of DAP3


  1. Death-associated protein 3 (Dap-3) is overexpressed in invasive glioblastoma cells in vivo and in glioma cell lines with induced motility phenotype in vitro. Mariani, L., Beaudry, C., McDonough, W.S., Hoelzinger, D.B., Kaczmarek, E., Ponce, F., Coons, S.W., Giese, A., Seiler, R.W., Berens, M.E. Clin. Cancer Res. (2001) [Pubmed]
  2. Mutational analysis of proapoptotic death associated protein 3 (DAP3) P-loop domain in common human carcinomas. Woo Lee, J., Hwa Soung, Y., Young Kim, S., Woo Nam, S., Sang Park, W., Young Lee, J., Jin Yoo, N., Hyung Lee, S. Acta oncologica (Stockholm, Sweden) (2006) [Pubmed]
  3. Interaction of DAP3 and FADD only after cellular disruption. Berger, T., Kretzler, M. Nat. Immunol. (2002) [Pubmed]
  4. A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins. Miyazaki, T., Reed, J.C. Nat. Immunol. (2001) [Pubmed]
  5. Structure-function analysis of an evolutionary conserved protein, DAP3, which mediates TNF-alpha- and Fas-induced cell death. Kissil, J.L., Cohen, O., Raveh, T., Kimchi, A. EMBO J. (1999) [Pubmed]
  6. Functional role of death-associated protein 3 (DAP3) in anoikis. Miyazaki, T., Shen, M., Fujikura, D., Tosa, N., Kim, H.R., Kon, S., Uede, T., Reed, J.C. J. Biol. Chem. (2004) [Pubmed]
  7. TRAIL-induced apoptosis is independent of the mitochondrial apoptosis mediator DAP3. Berger, T., Kretzler, M. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  8. Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia. Chu, P., Deforce, D., Pedersen, I.M., Kim, Y., Kitada, S., Reed, J.C., Kipps, T.J. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  9. Death-associated protein 3 localizes to the mitochondria and is involved in the process of mitochondrial fragmentation during cell death. Mukamel, Z., Kimchi, A. J. Biol. Chem. (2004) [Pubmed]
  10. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. Kissil, J.L., Deiss, L.P., Bayewitch, M., Raveh, T., Khaspekov, G., Kimchi, A. J. Biol. Chem. (1995) [Pubmed]
  11. mtDNA controls expression of the Death Associated Protein 3. Jacques, C., Chevrollier, A., Loiseau, D., Lagoutte, L., Savagner, F., Malthièry, Y., Reynier, P. Exp. Cell Res. (2006) [Pubmed]
  12. The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function. Hulkko, S.M., Wakui, H., Zilliacus, J. Biochem. J. (2000) [Pubmed]
  13. A new face on apoptosis: death-associated protein 3 and PDCD9 are mitochondrial ribosomal proteins. Cavdar Koc, E., Ranasinghe, A., Burkhart, W., Blackburn, K., Koc, H., Moseley, A., Spremulli, L.L. FEBS Lett. (2001) [Pubmed]
  14. Arg and DAP3 expression was correlated with human thymoma stage. Sasaki, H., Ide, N., Yukiue, H., Kobayashi, Y., Fukai, I., Yamakawa, Y., Fujii, Y. Clin. Exp. Metastasis (2004) [Pubmed]
  15. Functional interaction between the pro-apoptotic DAP3 and the glucocorticoid receptor. Hulkko, S.M., Zilliacus, J. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  16. Association between genetic variation in the gene for death-associated protein-3 (DAP3) and adult asthma. Hirota, T., Obara, K., Matsuda, A., Akahoshi, M., Nakashima, K., Hasegawa, K., Takahashi, N., Shimizu, M., Sekiguchi, H., Kokubo, M., Doi, S., Fujiwara, H., Miyatake, A., Fujita, K., Enomoto, T., Kishi, F., Suzuki, Y., Saito, H., Nakamura, Y., Shirakawa, T., Tamari, M. J. Hum. Genet. (2004) [Pubmed]
  17. Transcriptional activation in chondrocytes submitted to hydrostatic pressure. Sironen, R., Elo, M., Kaarniranta, K., Helminen, H.J., Lammi, M.J. Biorheology. (2000) [Pubmed]
  18. Assignment of death associated protein 3 (DAP3) to human chromosome 1q21 by in situ hybridization. Kissil, J.L., Kimchi, A. Cytogenet. Cell Genet. (1997) [Pubmed]
WikiGenes - Universities