High impact information on PRHA

• Here, we show that WUS encodes a novel homeodomain protein which presumably acts as a transcriptional regulator [1].
• Cloning of the BEL1 locus reveals that it encodes a homeodomain transcription factor [2].
• A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis [3].
• The KNOTTED class of plant genes encodes homeodomain proteins [3].
• The PRS gene, encoding a putative transcriptional factor with a homeodomain, was shown to be required for cell proliferation [4].

Biological context of PRHA

• Developmental and auxin-induced expression of the Arabidopsis prha homeobox gene [5].
• The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene [6].
• By analysing proteins capable of interacting with ABI1, we have identified the homeodomain protein ATHB6 as a regulator of the ABA signal pathway [7].
• The leader sequence of the isolated cDNA clone contains several small open reading frames upstream of the initiation codon of the largest open reading frame coding for the homeodomain protein [8].
• It is concluded that Arabidopsis encodes a large family of homeodomain proteins, including members that contain a homeodomain/leucine-zipper (HD-Zip) motif [9].

Anatomical context of PRHA

• In addition, the prha promoter is highly responsive to the synthetic auxin, naphthalene acetic acid, in transient assays using tobacco protoplasts [5].
• The Arabidopsis thaliana WUSCHEL gene (WUS), which encodes a homeodomain transcription factor, is required to maintain the stem cells in the shoot apical meristem in an undifferentiated state [10].
• AtOFP1 and AtOFP5 are shown to associate with the cytoskeleton and to regulate subcellular localization of TALE homeodomain proteins, suggesting a previously unrecognized control mechanism in plant development [11].

Associations of PRHA with chemical compounds

• Exogenously applied auxin strongly increased endogenous prha transcript levels [5].
• The Arabidopsis Athb-1 and -2 proteins are characterized by the presence of a homeodomain (HD) with a closely linked leucine zipper motif (Zip) [12].
• The endophytic fungus Piriformospora indica stimulates the expression of nitrate reductase and the starch-degrading enzyme glucan-water dikinase in tobacco and Arabidopsis roots through a homeodomain transcription factor that binds to a conserved motif in their promoters [13].
• HAT3.1, a novel Arabidopsis homeodomain protein containing a conserved cysteine-rich region [14].
• In addition, up-regulation of the homeodomain transcription factor FWA, transposon-related elements, and several DNA- and chromatin-modifying enzymes indicated that epigenetic changes contribute to the acquisition of cytokinin habituation [15].

Other interactions of PRHA

• The OCP3 gene encodes a homeodomain transcription factor that is constitutively expressed in healthy plants but repressed in response to infection by necrotrophic fungi [16].
• Sequencing of the ANL2 gene showed that it encodes a homeodomain protein belonging to the HD-GLABRA2 group [17].
• We isolated and characterized a cDNA designated ATML1 (for Arabidopsis thaliana meristem L1 layer) that encodes a novel homeodomain protein [18].
• Homeobox genes encode a large family of homeodomain proteins in animal systems [9].
• The WUS (WUSCHEL) gene, encoding a homeodomain protein, promotes the formation and maintenance of stem cells [19].

Analytical, diagnostic and therapeutic context of PRHA

• Gene mapping experiments and sequence comparison analysis revealed that Athb-10 corresponds to GLABRA2, a homeodomain protein involved in trichome development [20].
• Electrophoretic mobility shift assays demonstrated that the L1-specific homeodomain protein ATML1 can bind to the L1 box sequence in vitro [21].
• Inverse PCR and sequence analysis indicated that the enhancer-containing T-DNA from the activation tagging construct, SKI015, was inserted upstream of the previously cloned WUS gene encoding a homeodomain protein [22].
• Laser capture microdissection and reverse transcription-PCR were used to show that both the GhMyb25 and the homeodomain gene were predominantly ovule specific and were up-regulated on the day of anthesis in fibre initials relative to adjacent non-fibre ovule epidermal cells [23].

References