Disease relevance of PRSS27

• In marked contrast to prostasin, which is 43% identical in the catalytic domain, human pancreasin is transcribed strongly in pancreas (and in the pancreatic ductal adenocarcinoma line, HPAC) but weakly or not at all in kidney and prostate [1].
• Culturable E. coli occurred in 88% of the samples collected, with overall mean counts of 16 MPN g(-1), ranging from < 1 to 1657 (n = 66) [2].
• Coliphages were enumerated by a most-probable-number (MPN) procedure [3].
• Phylogenetic analysis indicated that isolate Milos-T1 is related to the recently described Thiomicrospira kuenenii and Hydrogenovibrio marinus, whereas isolate Milos-T2 grouped with the MPN sequences of cluster 2 [4].
• When PCR amplification was coupled with the MPN method, the counting rate reached 65 to 72% of inoculated Nitrobacter cells [5].

High impact information on PRSS27

• A mutation within the conserved MPN/JAMM domain (C120A) abolished the ability of HS13 to rescue the Drosophila cells from apoptosis or DNA overreplication [6].
• Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas [1].
• Like the other genes, the pancreasin gene resides on chromosome 16p [1].
• Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin [1].
• Sequencing of cDNA allowed us to deduce the full amino acid sequence of the human gene product, which we term "pancreasin" because it is transcribed strongly in the pancreas [1].

Chemical compound and disease context of PRSS27

• The MPN isolates were phylogenetically affiliated with sulfate-reducing delta subdivision proteobacteria (members of the genera Desulfovibrio, Desulfobulbus, and Desulfobacter), whereas the molecular isolates constituted an independent lineage of the delta subdivision proteobacteria [7].
• BACKGROUND: This study was designed to determine whether decreasing nasal bacterial colonization by applying Mupirocin (MPN) intranasally decreases sternal wound infections [8].
• Populations of Salmonella were determined by the most probable number (MPN) method, with isolation on bismuth sulfite agar plates [9].
• CONCLUSIONS: Disinfection with peracetic acid reduced levels of faecal contamination by 97%, thus attaining the limit recommended by current Italian law (Escherichia coli <or= 5000 MPN 100 ml(-1)) for discharge into surface waters [10].
• Serotype Newport was estimated at 0.07 MPN/100 g in lot A; the concentration for three serotypes was estimated to be 0.36 MPN/100 g in lot B; Salmonella group C1 was estimated at 1.8 MPN/100 g in lot C [11].

Biological context of PRSS27

• Pancreasin cDNA predicts a 290-residue, N-glycosylated, serine peptidase with a typical signal peptide, a 12-residue activation peptide cleaved by tryptic hydrolysis, and a 256-amino acid catalytic domain [1].
• Using the new MPN method, population estimates of pure cultures of DARB were similar to direct cell counts [12].
• These strains had identical 16S rRNA gene sequences and represented the dominant phylotype in the plateable fraction, as well as in the highest positive dilutions of the MPN series [13].
• The data from sediment incubation experiments, MPN series, and molecular-genetics detection correlated well and provide convincing evidence for the suggested mechanisms for MT and DMS cycling and the common presence of the DMS-degrading methanogen M. hollandica in freshwater sediments [14].
• The use of standard MPN techniques for monitoring potable water supplies may lead to a false security that the drinking water supply is potable, i.e., free from indicator bacteria [15].

Anatomical context of PRSS27

• More importantly, the alteration in PBMC GR-binding affinity with IL-2 + IL-4 was associated with a functional change in T cell response to methylprednisolone MPN, i.e., a reduced inhibitory effect of MPN on PMA/ionomycin-induced T cell proliferation [16].
• When results from the membranes with the highest total coliform recovery rate were compared with the MPN results, the MF results were found significantly higher in one experiment and equivalent to the MPN results in the other five experiments [17].
• After 48 hr, there was no significant difference (P < 0.05) between centrifuged and noncentrifuged samples enumerated by number of foci or the MPN of infectious oocysts [18].
• All three dipoles are located near central electrode positions, so the generators of the MPN probably reside within the motor cortex [19].
• In 75% the MPN arose from the anterior division of the inferior trunk of the brachial plexus and in 25% it arose from the medial cord [20].

Associations of PRSS27 with chemical compounds

• MPN counts for sulfate-reducing bacteria and dissimilatory iron-reducing bacteria ranged from 8 to 1.4 x 10(5) cells g(-1) and from undetectable to 1.4 x 10(6) cells g(-1), respectively [21].
• The presence of these bacteria in non-amended soil was also indicated by the most probable number (MPN) counts of nitrate-dependent iron(II) oxidizers in the layer of 3-4 mm soil depth, which revealed 1.6 x 10(6) bacteria g(-1) dry weight [22].
• Microbial phenanthrene degraders were not detected by MPN counting in the agricultural soil and the roadside soil [23].
• On the basis of most probable number (MPN) analyses, arsenite-oxidizing bacteria were found to account for 6-56% of the cultivated members of the community [24].
• The DGGE patterns of MPN cultures suggested that more organisms than those isolated thus far are able to oxidize ferrous iron with nitrate [25].

Analytical, diagnostic and therapeutic context of PRSS27

• Cell concentrations of ammonia oxidizers determined by MPN counts were between 10- and 100-fold lower than those determined by competitive PCR (cPCR) [26].
• Nine different brands of membrane filter were compared in the membrane filtration (MF) method, and those with the highest yields were compared against the most-probable-number (MPN) multiple-tube method for total coliform enumeration in simulated sewage-contaminated tap water [17].
• The temporal variation in archaeal diversity in vent fluids from a midocean ridge subseafloor habitat was examined using PCR-amplified 16S rRNA gene sequence analysis and most-probable-number (MPN) cultivation techniques targeting hyperthermophiles [27].
• This randomized, crossover, double-blind study compared the antiemetic efficacy of a single dose of 125 mg MPN with that of two such doses [28].
• We recommend the use of two doses of 125 mg MPN as prophylactic antiemetic treatment in breast-cancer patients receiving CMF chemotherapy [28].

References