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Gene Review

ESP1  -  Esp1p

Saccharomyces cerevisiae S288c

Synonyms: Separase, Separin, YGR098C
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Disease relevance of ESP1

  • The N-terminal half of ESP1, which is sufficient for CES2 function, displays local sequence similarity to the small subunit of the vaccinia virus RNA capping enzyme [1].
  • It also hinders extrusion of the first polar body (PBE) and causes female sterility. mRNA encoding wild-type but not catalytically inactive separase restores chiasma resolution [2].

High impact information on ESP1


Biological context of ESP1


Anatomical context of ESP1


Associations of ESP1 with chemical compounds


Enzymatic interactions of ESP1


Regulatory relationships of ESP1


Other interactions of ESP1

  • We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p [4].
  • Our results uncover a previously unknown mechanism for regulating the Pds1-Esp1 interaction and shed light on a novel role for Cdc28 in promoting the metaphase-to-anaphase transition in budding yeast [19].
  • Proteolytic cleavage of cohesin's Rec8 subunit by separase destroys cohesion between sister chromatid arms at anaphase I and thereby resolves chiasmata [20].
  • Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14 [21].
  • Here we show that separase also cleaves the kinetochore-associated protein Slk19 at anaphase onset [22].


  1. Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Schwer, B., Shuman, S. Gene Expr. (1996) [Pubmed]
  2. Resolution of chiasmata in oocytes requires separase-mediated proteolysis. Kudo, N.R., Wassmann, K., Anger, M., Schuh, M., Wirth, K.G., Xu, H., Helmhart, W., Kudo, H., McKay, M., Maro, B., Ellenberg, J., de Boer, P., Nasmyth, K. Cell (2006) [Pubmed]
  3. Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast. Queralt, E., Lehane, C., Novak, B., Uhlmann, F. Cell (2006) [Pubmed]
  4. An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast. Ciosk, R., Zachariae, W., Michaelis, C., Shevchenko, A., Mann, M., Nasmyth, K. Cell (1998) [Pubmed]
  5. Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage. Tinker-Kulberg, R.L., Morgan, D.O. Genes Dev. (1999) [Pubmed]
  6. The Saccharomyces cerevisiae PDS1 and RAD9 checkpoint genes control different DNA double-strand break repair pathways. DeMase, D., Zeng, L., Cera, C., Fasullo, M. DNA Repair (Amst.) (2005) [Pubmed]
  7. Uncovering novel cell cycle players through the inactivation of securin in budding yeast. Sarin, S., Ross, K.E., Boucher, L., Green, Y., Tyers, M., Cohen-Fix, O. Genetics (2004) [Pubmed]
  8. Cohesin ensures bipolar attachment of microtubules to sister centromeres and resists their precocious separation. Tanaka, T., Fuchs, J., Loidl, J., Nasmyth, K. Nat. Cell Biol. (2000) [Pubmed]
  9. Separase is required at multiple pre-anaphase cell cycle stages in human cells. Giménez-Abián, J.F., Díaz-Martínez, L.A., Waizenegger, I.C., Giménez-Martín, G., Clarke, D.J. Cell Cycle (2005) [Pubmed]
  10. The anaphase-promoting complex and separin are required for embryonic anterior-posterior axis formation. Rappleye, C.A., Tagawa, A., Lyczak, R., Bowerman, B., Aroian, R.V. Dev. Cell (2002) [Pubmed]
  11. Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast. Salah, S.M., Nasmyth, K. Chromosoma (2000) [Pubmed]
  12. Role of the kinetochore protein Ndc10 in mitotic checkpoint activation in Saccharomyces cerevisiae. Fraschini, R., Beretta, A., Lucchini, G., Piatti, S. Mol. Genet. Genomics (2001) [Pubmed]
  13. Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast. Uhlmann, F., Wernic, D., Poupart, M.A., Koonin, E.V., Nasmyth, K. Cell (2000) [Pubmed]
  14. Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase. Penkner, A.M., Prinz, S., Ferscha, S., Klein, F. Cell (2005) [Pubmed]
  15. Studies on substrate recognition by the budding yeast separase. Sullivan, M., Hornig, N.C., Porstmann, T., Uhlmann, F. J. Biol. Chem. (2004) [Pubmed]
  16. Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase. Waizenegger, I.C., Hauf, S., Meinke, A., Peters, J.M. Cell (2000) [Pubmed]
  17. A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1. Jensen, S., Segal, M., Clarke, D.J., Reed, S.I. J. Cell Biol. (2001) [Pubmed]
  18. Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregation. Higuchi, T., Uhlmann, F. Nature (2005) [Pubmed]
  19. Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase. Agarwal, R., Cohen-Fix, O. Genes Dev. (2002) [Pubmed]
  20. Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19. Buonomo, S.B., Rabitsch, K.P., Fuchs, J., Gruber, S., Sullivan, M., Uhlmann, F., Petronczki, M., Tóth, A., Nasmyth, K. Dev. Cell (2003) [Pubmed]
  21. Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Pereira, G., Schiebel, E. Science (2003) [Pubmed]
  22. Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19. Sullivan, M., Lehane, C., Uhlmann, F. Nat. Cell Biol. (2001) [Pubmed]
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