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Gene Review

dcm  -  DNA cytosine methyltransferase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1959, JW1944, mec
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Disease relevance of dcm


High impact information on dcm


Chemical compound and disease context of dcm


Biological context of dcm


Associations of dcm with chemical compounds

  • The methylase within the EcoRII system methylates the same cytosine as the dcm protein [2].
  • However, mismatches in heteroduplexes prepared from lambda DNA lacking 5-methylcytosine were repaired in dcm+ bacteria [9].
  • High levels of AdoMetase expression impaired AdoMet-mediated activities such as dam and dcm methylase-directed DNA modifications and the synthesis of spermidine from putrescine [16].
  • In contrast, Tn3 was found to transpose more frequently in dcm+ (deoxycytosine methylase) cells than in dcm- mutants [17].
  • Hybridization of this 3.5-kilobase Bg/II fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant (mec) [18].

Other interactions of dcm

  • Based on these studies we conclude that there is a six-codon overlap between vsr and dcm [15].

Analytical, diagnostic and therapeutic context of dcm


  1. Nucleotide sequence of the dcm locus of Escherichia coli K12. Hanck, T., Gerwin, N., Fritz, H.J. Nucleic Acids Res. (1989) [Pubmed]
  2. Cloning and characterization of the dcm locus of Escherichia coli K-12. Bhagwat, A.S., Sohail, A., Roberts, R.J. J. Bacteriol. (1986) [Pubmed]
  3. Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes. Dreiseikelmann, B., Wackernagel, W. J. Bacteriol. (1981) [Pubmed]
  4. DNA methylation pattern is determined by the intracellular level of the methylase. Szyf, M., Avraham-Haetzni, K., Reifman, A., Shlomai, J., Kaplan, F., Oppenheim, A., Razin, A. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  5. Partially deficient methylation of cytosine in DNA at CCATGG sites stimulates genetic recombination of bacteriophage lambda. Korba, B.E., Hays, J.B. Cell (1982) [Pubmed]
  6. DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues. Zell, R., Fritz, H.J. EMBO J. (1987) [Pubmed]
  7. The Escherichia coli chromosome contains specific, unmethylated dam and dcm sites. Ringquist, S., Smith, C.L. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  8. Very short patch repair: reducing the cost of cytosine methylation. Lieb, M., Bhagwat, A.S. Mol. Microbiol. (1996) [Pubmed]
  9. Bacterial genes mutL, mutS, and dcm participate in repair of mismatches at 5-methylcytosine sites. Lieb, M. J. Bacteriol. (1987) [Pubmed]
  10. Sequence-specific and mechanism-based crosslinking of Dcm DNA cytosine-C5 methyltransferase of E. coli K-12 to synthetic oligonucleotides containing 5-fluoro-2'-deoxycytidine. Hanck, T., Schmidt, S., Fritz, H.J. Nucleic Acids Res. (1993) [Pubmed]
  11. DNA binding and cleavage selectivity of the Escherichia coli DNA G:T-mismatch endonuclease (vsr protein). Gonzalez-Nicieza, R., Turner, D.P., Connolly, B.A. J. Mol. Biol. (2001) [Pubmed]
  12. The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli sn-glycerophosphoryl diester phosphodiesterase. Ryffel, C., Bucher, R., Kayser, F.H., Berger-Bächi, B. J. Bacteriol. (1991) [Pubmed]
  13. DNA methylation of bacterial viruses T3 and T7 by different DNA methylases in Escherichia coli K12 cells. Krüger, D.H., Schroeder, C., Reuter, M., Bogdarina, I.G., Buryanov, Y.I., Bickle, T.A. Eur. J. Biochem. (1985) [Pubmed]
  14. A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene. Sohail, A., Lieb, M., Dar, M., Bhagwat, A.S. J. Bacteriol. (1990) [Pubmed]
  15. Mechanism of expression of DNA repair gene vsr, an Escherichia coli gene that overlaps the DNA cytosine methylase gene, dcm. Dar, M.E., Bhagwat, A.S. Mol. Microbiol. (1993) [Pubmed]
  16. Expression of the cloned coliphage T3 S-adenosylmethionine hydrolase gene inhibits DNA methylation and polyamine biosynthesis in Escherichia coli. Hughes, J.A., Brown, L.R., Ferro, A.J. J. Bacteriol. (1987) [Pubmed]
  17. Involvement of E. coli dcm methylase in Tn3 transposition. Yang, M.K., Ser, S.C., Lee, C.H. Proc. Natl. Sci. Counc. Repub. China B (1989) [Pubmed]
  18. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. Beck, W.D., Berger-Bächi, B., Kayser, F.H. J. Bacteriol. (1986) [Pubmed]
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