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Gene Review

alkB  -  oxidative demethylase of N1-methyladenine...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2204, JW2200, aidD
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Disease relevance of alkB


High impact information on alkB


Chemical compound and disease context of alkB


Biological context of alkB


Anatomical context of alkB


Associations of alkB with chemical compounds

  • Under aerobic conditions in the absence of alkylating agents aidB, but not alkA or alkB, was induced by an acidification of cytoplasm or by treatment with the sulfhydryl reagent N-ethylmaleimide [16].
  • Furthermore, we found that alkS is responsible for activation of expression of the alkBAC operon since it is required for the induction of the alkB gene product alkane hydroxylase [17].
  • We constructed hybrid plasmids carrying an alkB'-lacZ' fusion, with or without the ada control region, and investigated expression of the alkB gene in response to the alkylating agent [11].
  • When hABH was expressed in E.coli alkB mutant cells partial rescue of the cells from MMS-induced cell death occurred [18].
  • Also, alkB mutants were not sensitive to MeOSO2-(CH2)2-Lex, a compound that alkylates the minor groove of DNA generating primarily 3-methyladenine [19].

Other interactions of alkB

  • Another mutation was found near nalA, and the gene responsible was named alkB [9].

Analytical, diagnostic and therapeutic context of alkB


  1. Defective processing of methylated single-stranded DNA by E. coli AlkB mutants. Dinglay, S., Trewick, S.C., Lindahl, T., Sedgwick, B. Genes Dev. (2000) [Pubmed]
  2. The Pseudomonas oleovorans alkane hydroxylase gene. Sequence and expression. Kok, M., Oldenhuis, R., van der Linden, M.P., Raatjes, P., Kingma, J., van Lelyveld, P.H., Witholt, B. J. Biol. Chem. (1989) [Pubmed]
  3. An alkB gene homolog is differentially transcribed during the Caulobacter crescentus cell cycle. Colombi, D., Gomes, S.L. J. Bacteriol. (1997) [Pubmed]
  4. Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains. Smits, T.H., Röthlisberger, M., Witholt, B., van Beilen, J.B. Environ. Microbiol. (1999) [Pubmed]
  5. AlkB mystery solved: oxidative demethylation of N1-methyladenine and N3-methylcytosine adducts by a direct reversal mechanism. Begley, T.J., Samson, L.D. Trends Biochem. Sci. (2003) [Pubmed]
  6. Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli. Delaney, J.C., Essigmann, J.M. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  7. Reversal of DNA alkylation damage by two human dioxygenases. Duncan, T., Trewick, S.C., Koivisto, P., Bates, P.A., Lindahl, T., Sedgwick, B. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  8. Alkylating agents induce UVM, a recA-independent inducible mutagenic phenomenon in Escherichia coli. Wang, G., Palejwala, V.A., Dunman, P.M., Aviv, D.H., Murphy, H.S., Rahman, M.S., Humayun, M.Z. Genetics (1995) [Pubmed]
  9. A new gene (alkB) of Escherichia coli that controls sensitivity to methyl methane sulfonate. Kataoka, H., Yamamoto, Y., Sekiguchi, M. J. Bacteriol. (1983) [Pubmed]
  10. A second DNA methyltransferase repair enzyme in Escherichia coli. Rebeck, G.W., Coons, S., Carroll, P., Samson, L. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  11. Structure and expression of the alkB gene of Escherichia coli related to the repair of alkylated DNA. Kondo, H., Nakabeppu, Y., Kataoka, H., Kuhara, S., Kawabata, S., Sekiguchi, M. J. Biol. Chem. (1986) [Pubmed]
  12. Suppression of Escherichia coli alkB mutants by Saccharomyces cerevisiae genes. Wei, Y.F., Chen, B.J., Samson, L. J. Bacteriol. (1995) [Pubmed]
  13. Molecular cloning and characterization of the alkB gene of Escherichia coli. Kataoka, H., Sekiguchi, M. Mol. Gen. Genet. (1985) [Pubmed]
  14. Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes. Samson, L., Derfler, B., Waldstein, E.A. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  15. Determinants for overproduction of the Pseudomonas oleovorans cytoplasmic membrane protein alkane hydroxylase in alk+ Escherichia coli W3110. Nieboer, M., Gunnewijk, M., van Beilen, J.B., Witholt, B. J. Bacteriol. (1997) [Pubmed]
  16. Induction of the alkylation-inducible aidB gene of Escherichia coli by cytoplasmic acidification and N-ethylmaleimide. Smirnova, G.V., Oktyabrsky, O.N., Moshonkina, E.V., Zakirova, N.V. Mutat. Res. (1994) [Pubmed]
  17. Alkane utilization in Pseudomonas oleovorans. Structure and function of the regulatory locus alkR. Eggink, G., Engel, H., Meijer, W.G., Otten, J., Kingma, J., Witholt, B. J. Biol. Chem. (1988) [Pubmed]
  18. Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair. Wei, Y.F., Carter, K.C., Wang, R.P., Shell, B.K. Nucleic Acids Res. (1996) [Pubmed]
  19. Repair in Escherichia coli alkB mutants of abasic sites and 3-methyladenine residues in DNA. Dinglay, S., Gold, B., Sedgwick, B. Mutat. Res. (1998) [Pubmed]
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