Disease relevance of Parvovirus, Porcine

• A solid phase fluorescent immunoassay using polyacrylamide beads coated with rabbit anti-porcine parvovirus antibodies has been developed and utilized in the diagnosis of porcine parvovirus infection [1].
• The time for inactivation of 7 log10 units of porcine parvovirus (PPV) was about 24 h for both El concentrations, whereas 5 log10 units of bovine viral diarrhea virus (BVDV) was inactivated in 2 h for both El concentrations and 6 log10 units of porcine enterovirus (PEV) was inactivated within 3 h [2].
• The involvement of porcine parvovirus (PPV) in the aetiology of non-suppurative myocarditis in sucking piglets was investigated by a polymerase chain reaction (PCR), designed to assess the presence of viral genome in formalin-fixed paraffin wax-embedded tissue of diseased animals [3].
• Specific primers for each of four common DNA viruses, namely, pseudorabies virus (PRV), porcine circovirus type I (PCV1), porcine circovirus type II (PCV2), and porcine parvovirus (PPV), were used for testing procedure [4].
• In Exp. 3, 36 weaned piglets (28 d of age) were fed a common diet for 14 d and were determined to be negative for PRV, PRRS, and porcine parvovirus titer [5].

High impact information on Parvovirus, Porcine

• Here, we analyzed the contribution of the porcine parvovirus (PPV)-VLPs to the induction of protective cellular responses to exogenous Ags carried by an independent delivery system [6].
• Incubation of mammalian cells with porcine parvovirus PLA(2) led to the release of arachidonic acid into the culture medium [7].
• Dry-heat sensitivity of human B19 and porcine parvoviruses [8].
• The 3'-terminal 853 nt (and the putative 283 aa) sequence of the VP2-encoding gene from 29 field strains of porcine parvovirus (PPV) were determined and compared both to each other and with other published sequences [9].
• The NS and capsid genes determine the host range of porcine parvovirus [10].

Chemical compound and disease context of Parvovirus, Porcine

• The effectiveness of experimental squalene adjuvant was tested using a liquid inactivated adjuvant rabies vaccine and a liquid porcine parvovirus vaccine in comparison with adjuvant-free vaccines or vaccines potentiated with aluminium hydroxide [11].
• Nonradioactive slot blot hybridization assays were established for the detection of porcine parvovirus (PPV), using either a digoxigenin-labeled DNA probe or a biotinylated RNA probe [12].
• Of a variety of disinfectants evaluated, only sodium hypochlorite and sodium hydroxide inactivated porcine parvovirus (PPV) after a 5-minute incubation period [13].
• Cell culture fluids containing porcine parvovirus were adjuvanted with varying concentrations of aluminum hydroxide gel [14].
• A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus [15].

Biological context of Parvovirus, Porcine

• Nucleotide sequence analysis of the capsid genes and the right-hand terminal palindrome of porcine parvovirus, strain NADL-2 [16].
• The humoral antibody response against the nonstructural protein NS1 and the structural protein VP2 of porcine parvovirus (PPV) was evaluated by immuno-peroxidase test (IPT) and enzyme linked immuno sorbent assay (ELISA) using recombinant PPV antigens [17].
• Desorption of porcine parvovirus from aluminum hydroxide adjuvant with subsequent viral immunoassay or hemagglutination assay [14].

Gene context of Parvovirus, Porcine

• Porcine parvovirus (PPV) vaccines containing different adjuvants were evaluated for inducing Th1 or Th2 type of immunity in mice [18].
• In contrast, porcine parvovirus was widely resistant at 60 degrees C. Inactivation of B19 was independent of the analyzed albumin products (5, 20, and 25% albumin from three manufacturers) and from the specific virus source used for the inactivation experiments [19].
• Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs [17].
• The antigenic structure of the capsid proteins of porcine parvovirus (PPV) was investigated [20].
• This report describes a real-time polymerase chain reaction assay with SYBR((R)) Green for detection of a broad range of porcine parvoviruses (PPV) and accurate virus quantification in porcine tissues [21].

References