The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Chemical Compound Review

AC1LCGAJ     2-(4-iodophenyl)-3-(4- nitrophenyl)-5...

Synonyms: PubChem20100, CHEMBL272094, SureCN170315, SureCN342687, CCRIS 4228, ...
This record was replaced with 64958.
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of Iodonitrotetrazolium purple

 

High impact information on Iodonitrotetrazolium purple

  • In contrast, the D484T and D500A/R/G mutants of the alpha-helical loop of Nox2 exhibited no NADPH oxidase and INT reductase activities associated with a defective p47(phox) membrane translocation [2].
  • However, these D-loop mutants demonstrated normal p47(phox) translocation and iodonitrotetrazolium (INT) reductase activity, suggesting that charged amino acids of this region are essential for electron transfer from FAD to oxygen [2].
  • The reduced flavin exhibits normal levels of iodonitrotetrazolium violet diaphorase activity, and the patient's neutrophils exhibit high levels of intracellular oxidant production and show a low level of NBT staining in the NBT slide test [3].
  • Furthermore, peritoneal macrophages from endrin-treated animals resulted in 3.0- and 2.8-fold increases in cytochrome c and iodonitrotetrazolium (INT) reduction, indicating enhanced production of superoxide anion [4].
  • In a cell-free system, DEPC-treated membranes failed to support superoxide generation or the reduction of Iodonitrotetrazolium Violet and showed a loss of the characteristic cytochrome b(558) spectrum [5].
 

Biological context of Iodonitrotetrazolium purple

 

Anatomical context of Iodonitrotetrazolium purple

 

Associations of Iodonitrotetrazolium purple with other chemical compounds

 

Gene context of Iodonitrotetrazolium purple

  • Although p-iodonitrotetrazolium (INT) has frequently been used as a probe of the diaphorase activity of the neutrophil flavocytochrome b, the propensity of its radical to interact reversibly with O2 led us to question its specificity [7].
  • No such linear free energy relationship was found between redox potential and activity with ferredoxin or iodonitrotetrazolium as acceptor [19].
 

Analytical, diagnostic and therapeutic context of Iodonitrotetrazolium purple

  • Second, the titration curve of dithionite-reduced heme b in neutrophil membranes obtained by oxidation with increasing amounts of INT was strictly superimposable on that of dithionite-reduced hemin [7].

References

  1. A superoxide-producing system in the conjunctival mucus thread. Proctor, P., Kirkpatrick, D., McGinness, J. Invest. Ophthalmol. Vis. Sci. (1977) [Pubmed]
  2. Crucial role of two potential cytosolic regions of Nox2, 191TSSTKTIRRS200 and 484DESQANHFAVHHDEEKD500, on NADPH oxidase activation. Li, X.J., Grunwald, D., Mathieu, J., Morel, F., Stasia, M.J. J. Biol. Chem. (2005) [Pubmed]
  3. A variant X-linked chronic granulomatous disease patient (X91+) with partially functional cytochrome b. Cross, A.R., Heyworth, P.G., Rae, J., Curnutte, J.T. J. Biol. Chem. (1995) [Pubmed]
  4. Production of reactive oxygen species by peritoneal macrophages and hepatic mitochondria and microsomes from endrin-treated rats. Bagchi, M., Hassoun, E.A., Bagchi, D., Stohs, S.J. Free Radic. Biol. Med. (1993) [Pubmed]
  5. Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying agent: evidence for at least two target sites. Mankelow, T.J., Henderson, L.M. Biochem. J. (2001) [Pubmed]
  6. Cellular localization of the inhibitory action of abruquinone A against respiratory burst in rat neutrophils. Hsu, M.F., Raung, S.L., Tsao, L.T., Kuo, S.C., Wang, J.P. Br. J. Pharmacol. (1997) [Pubmed]
  7. Exploration of the diaphorase activity of neutrophil NADPH oxidase. Poinas, A., Gaillard, J., Vignais, P., Doussiere, J. Eur. J. Biochem. (2002) [Pubmed]
  8. In vitro chemosensitivity testing of leukemic cells: development of a semiautomated colorimetric assay. Bernabei, P.A., Santini, V., Silvestro, L., Dal Pozzo, O., Bezzini, R., Viano, I., Gattei, V., Saccardi, R., Rossi Ferrini, P. Hematological oncology. (1989) [Pubmed]
  9. Vaginal leukocyte characteristics in urogenital trichomoniasis. Buchvald, D., Demes, P., Gombosová, A., Mráz, P., Valent, M., Stefanovic, J. APMIS (1992) [Pubmed]
  10. The NADPH oxidase diaphorase activity in permeabilized human neutrophils and granulocytic like PLB-985 cells. Pessach, I., Levy, R. Adv. Exp. Med. Biol. (2000) [Pubmed]
  11. Papain proteolysis releases a soluble NADPH dependent diaphorase activity from bovine neutrophil membranes. Li, J., Kon, L.M., Guillory, R.J. FEBS Lett. (1998) [Pubmed]
  12. ATP-induced release of vasopressin associated with phosphorylation of isolated bovine neurohypophyseal secretory granule membranes. Russell, J.T., Thorn, N.A. Acta Endocrinol. (1978) [Pubmed]
  13. Lysosomal enzymes and superoxide production in polymorphonuclear leukocytes of patients with primary varicose veins. Stvrtinová, V., Ferencíková, J. Cor et vasa. (1992) [Pubmed]
  14. Purified leukocyte cytochrome b558 incorporated into liposomes catalyzes a cytosolic factor dependent diaphorase activity. Li, J., Guillory, R.J. Biochemistry (1997) [Pubmed]
  15. Characteristics of a new urine, serum, and saliva alcohol reagent strip. Tu, G.C., Kapur, B., Israel, Y. Alcohol. Clin. Exp. Res. (1992) [Pubmed]
  16. Monitoring of phenylketonuria: a colorimetric method for the determination of plasma phenylalanine using L-phenylalanine dehydrogenase. Wendel, U., Hummel, W., Langenbeck, U. Anal. Biochem. (1989) [Pubmed]
  17. Isolation of synaptic junction-enriched fraction from the forebrain of day-old chickens. Preparation and characterization of chick forebrain subcellular fractions. Webster, J.C., Klingman, J.D. Neurochem. Res. (1979) [Pubmed]
  18. Colorimetric determination of galactose and galactose-1-phosphate from dried blood. Diepenbrock, F., Heckler, R., Schickling, H., Engelhard, T., Bock, D., Sander, J. Clin. Biochem. (1992) [Pubmed]
  19. FAD analogues as mechanistic and 'binding-domain' probes of spinach ferredoxin-NADP+ reductase. Zanetti, G., Massey, V., Curti, B. Eur. J. Biochem. (1983) [Pubmed]
 
WikiGenes - Universities