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Chemical Compound Review

SureCN18746     2-(4-carboxyquinolin-2- yl)quinoline-4...

Synonyms: CHEMBL431482, AG-D-52303, AG-K-08023, SureCN9410760, ANW-43691, ...
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Disease relevance of NSC133815

  • Our objectives were 2-fold: 1) to assess the concentration and distribution of total protein in human amniotic fluid (AF) using 3 standard assays [Bradford, bicinchoninic acid solution (BCA), and Lowry] and 2) to establish whether these total protein concentrations were associated with and predictive of infant birth weight [1].
  • RESULTS: The L-NAME group showed increased MPO activity in the lung compared with the NS group (mean MPO/mean bicinchoninic acid [BCA]: 43.46 +/- 3.10 U/microg v 23.58 +/- 2.48 U/microg; mean MPO/g wet lung [gwl]: 57.60 +/- 5.98 U/gwl v 27.10 +/- 3.84 U/gwl, mean +/- SEM; P <.05) [2].

High impact information on NSC133815

  • Enzyme-linked immunosorbent assays were used to determine fibronectin and albumin concentrations, and bicinchoninic acid protein assays were used to determine total protein concentration in each sample [3].
  • PSMA was purified from LNCaP cells by immunoaffinity chromatography, and used as a calibrator, based on its concentration by the bicinchoninic acid (BCA) protein assay [4].
  • The intensity of SYPRO-protein fluorescence of these bacteria strongly correlated with their total protein content, measured by the bicinchoninic acid method to be in the range of 60 to 330 fg of protein cell-1 (r2 = 0.93, n = 34) [5].
  • Whole milk, decelled milk, skimmed milk and whey were analyzed for protein with the Lowry method, modified for fat-containing samples, the Bradford dye-binding assay (Bio-Rad) and the Pierce bicinchoninic acid (BCA) assay [6].
  • The cell number could then be assessed either by methylene blue staining or by dissolving the cells in NaOH and estimating the protein content with bicinchoninic acid [7].

Biological context of NSC133815


Anatomical context of NSC133815


Associations of NSC133815 with other chemical compounds

  • We found that the cuprous ion formed in this type of redox reaction could be detected and quantified using 2,2'-bicinchoninic acid [17].
  • 24-h urinary total protein excretion in men was 91.2 +/- 25.1 mg (mean +/- SD) (range: 40.2-146.8 mg), while that in women was 62.5 +/- 23.6 mg (range: 28.4-130.9 mg), as measured by a biuret method using bicinchoninic acid as a reagent [18].
  • The concentration of a recombinant interferon gamma receptor produced in two differently glycosylated forms was underestimated by the Coomassie blue assay and overestimated by the bicinchoninic acid and Lowry methods, while for the non-glycosylated form of the same protein, the three colorimetric methods delivered comparable values [19].
  • A photometric pyrogallol-red molybdate complex method was found to correlate well with this HPLC method and with the BCA method only for tissue homogenate [20].

Gene context of NSC133815

  • The levels of MIF, IL-1beta, TNF-alpha, and RANTES were determined by enzyme-linked immunosorbent assay, and the concentrations of endotoxin and total protein were determined by the Endospec assay and bicinchoninic acid assay, respectively [21].
  • Linear standard curves were obtained for 1 to 10 micrograms of bovine serum albumin measured in the presence of 50 mM Mes, Mops, Pipes, Caps, Bes, Hepes, Heppso, and Tes zwitterionic buffers using bicinchoninic acid for detection [22].
  • In the bicinchoninic acid method, the detergent caused a 2- to 3-fold increase in OD562 due largely to contaminating peroxides which could be removed by treatment with catalase [23].
  • An alkaloidal extract of the vines of Stephania japonica showed multidrug-resistance-reversing activity as demonstrated by the bicinchoninic acid assay [24].
  • Finally, the same experiment was repeated, monitoring lysozyme deposition using the BCA assay [25].

Analytical, diagnostic and therapeutic context of NSC133815


  1. Total protein concentration in human amniotic fluid is negatively associated with infant birth weight. Tisi, D.K., Emard, J.J., Koski, K.G. J. Nutr. (2004) [Pubmed]
  2. Blocking of endogenous nitric oxide increases white blood cell accumulation in rat lung. Roman, A., Legallo, R., McGahren, E.D. J. Pediatr. Surg. (2004) [Pubmed]
  3. Fibronectin in the tear film. Fukuda, M., Fullard, R.J., Willcox, M.D., Baleriola-Lucas, C., Bestawros, F., Sweeney, D., Holden, B.A. Invest. Ophthalmol. Vis. Sci. (1996) [Pubmed]
  4. A dual-monoclonal sandwich assay for prostate-specific membrane antigen: levels in tissues, seminal fluid and urine. Sokoloff, R.L., Norton, K.C., Gasior, C.L., Marker, K.M., Grauer, L.S. Prostate (2000) [Pubmed]
  5. Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry. Zubkov, M.V., Fuchs, B.M., Eilers, H., Burkill, P.H., Amann, R. Appl. Environ. Microbiol. (1999) [Pubmed]
  6. Compartmentalization and quantitation of protein in human milk. Lönnerdal, B., Woodhouse, L.R., Glazier, C. J. Nutr. (1987) [Pubmed]
  7. A semiautomated, 96-well plate assay for collagen synthesis. Scutt, A., Berg, A., Mayer, H. Anal. Biochem. (1992) [Pubmed]
  8. Spectrophotometric quantitation of anchorage-dependent cell numbers using the bicinchoninic acid protein assay reagent. Tuszynski, G.P., Murphy, A. Anal. Biochem. (1990) [Pubmed]
  9. Protein analysis with bicinchoninic acid. Shihabi, Z.K., Dyer, R.D. Ann. Clin. Lab. Sci. (1988) [Pubmed]
  10. Breast milk from mothers of very low birthweight infants: variability in fat and protein content. Weber, A., Loui, A., Jochum, F., Bührer, C., Obladen, M. Acta Paediatr. (2001) [Pubmed]
  11. Effects of long-term sub-lethal concentrations of dental monomers on THP-1 human monocytes. Lefebvre, C.A., Wataha, J.C., Bouillaguet, S., Lockwood, P.E. Journal of biomaterials science. Polymer edition. (1999) [Pubmed]
  12. Protein analysis of mammalian cells in monolayer culture using the bicinchoninic assay. Goldschmidt, R.C., Kimelberg, H.K. Anal. Biochem. (1989) [Pubmed]
  13. Total protein in common duct bile measured by acetonitrile precipitation and a micro bicinchoninic acid (BCA) method. Osnes, T., Sandstad, O., Skar, V., Osnes, M., Kierulf, P. Scand. J. Clin. Lab. Invest. (1993) [Pubmed]
  14. Nitric oxide and cyclic GMP-mediated protein secretion from cultured lacrimal gland acinar cells. Beauregard, C., Brandt, P.C., Chiou, G.C. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics. (2002) [Pubmed]
  15. Soluble tumour necrosis factor receptors in synovial fluids from temporomandibular joints with painful anterior disc displacement without reduction and osteoarthritis. Uehara, J., Kuboki, T., Fujisawa, T., Kojima, S., Maekawa, K., Yatani, H. Arch. Oral Biol. (2004) [Pubmed]
  16. Methylation of the insulin-like growth factor binding protein-3 gene and prognosis of epithelial ovarian cancer. Wiley, A., Katsaros, D., Fracchioli, S., Yu, H. Int. J. Gynecol. Cancer (2006) [Pubmed]
  17. Quantitative benedict test using bicinchoninic acid. Chen, Q., Klemm, N., Jeng, I.M. Anal. Biochem. (1989) [Pubmed]
  18. Normal values for 24-h urinary protein excretion: total and low molecular weight proteins with a sex-related difference. Kawakami, H., Murakami, T., Kajii, T. Clin. Nephrol. (1990) [Pubmed]
  19. Comparison of the Coomassie brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-glycosylated and glycosylated proteins. Fountoulakis, M., Juranville, J.F., Manneberg, M. J. Biochem. Biophys. Methods (1992) [Pubmed]
  20. Protein determination by high-performance gel-permeation chromatography: applications to human pancreatic juice, human bile and tissue homogenate. Hayakawa, K., Yoshinaga, T., Hirano, M., Yoshikawa, K., Katsumata, N., Tanaka, T., Nagamine, T. J. Chromatogr. B Biomed. Sci. Appl. (2001) [Pubmed]
  21. Role of macrophage migration inhibitory factor in otitis media with effusion in adults. Kariya, S., Okano, M., Aoji, K., Kosaka, M., Chikumoto, E., Hattori, H., Yuen, K., Nishioka, S., Nishioka, K., Nishizaki, K. Clin. Diagn. Lab. Immunol. (2003) [Pubmed]
  22. Effect of zwitterionic buffers on measurement of small masses of protein with bicinchoninic acid. Kaushal, V., Barnes, L.D. Anal. Biochem. (1986) [Pubmed]
  23. Interference of the detergent Tween 80 in protein assays. Stutzenberger, F.J. Anal. Biochem. (1992) [Pubmed]
  24. Multidrug-resistance modulators from Stephania japonica. Hall, A.M., Chang, C.J. J. Nat. Prod. (1997) [Pubmed]
  25. Adhesion of tear proteins to contact lenses and vials. Keith, E.O., Boltz, M., Gadh, R., Ghorsriz, R., Mangatt, D., Janoff, L.E. Biotechnol. Appl. Biochem. (2001) [Pubmed]
  26. Quantitative analysis of relative protein contents by Western blotting: comparison of three members of the dystrophin-glycoprotein complex in slow and fast rat skeletal muscle. Chopard, A., Pons, F., Charpiot, P., Marini, J.F. Electrophoresis (2000) [Pubmed]
  27. Protein release profiles and morphology of biodegradable microcapsules containing an oily core. Youan, B.B., Jackson, T.L., Dickens, L., Hernandez, C., Owusu-Ababio, G. Journal of controlled release : official journal of the Controlled Release Society. (2001) [Pubmed]
  28. Determination of protein loading in biodegradable polymer microspheres containing tetanus toxoid. Gupta, R.K., Chang, A.C., Griffin, P., Rivera, R., Guo, Y.Y., Siber, G.R. Vaccine (1997) [Pubmed]
  29. Measurement of protein using flow injection analysis with bicinchoninic acid. Davis, L.C., Radke, G.A. Anal. Biochem. (1987) [Pubmed]
  30. Elevated levels of beta-endorphin in temporomandibular joint synovial lavage fluid of patients with closed lock. Kajii, T.S., Okamoto, T., Yura, S., Mabuchi, A., Iida, J. Journal of orofacial pain. (2005) [Pubmed]
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