Disease relevance of c1546

• Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo [1].
• Coordinated Regulation of the Neisseria gonorrhoeae-truncated Denitrification Pathway by the Nitric Oxide-sensitive Repressor, NsrR, and Nitrite-insensitive NarQ-NarP [2].
• LfrR Is a Repressor That Regulates Expression of the Efflux Pump LfrA in Mycobacterium smegmatis [3].
• Cloning and characterization of SmeT, a repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF [4].
• However, the betaproteobacteria primarily utilize the HrcA repressor protein to control genes involved in the stress response [5].

High impact information on c1546

• Regulation of transcription initiation is generally attributable to activator/repressor proteins that bind to specific DNA sequences [6].
• We consider an example of an SOS repair system, and computationally infer the regulator activity of its master repressor, LexA [7].
• In this work, a structure determined by x-ray crystallography of a complex of the repressor bound to biotin, which also functions as an activator of DNA binding by the biotin repressor (BirA), is described [8].
• The results suggest that the corepressor of BirA causes a disorder-to-order transition that is a prerequisite to repressor dimerization and DNA binding [8].
• ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli [9].

Chemical compound and disease context of c1546

• Transcription of the biotin (bio) biosynthetic operon of Escherichia coli is negatively regulated by the BirA protein, an atypical repressor protein in that it is also an enzyme [10].
• Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain [11].
• Transcriptional regulator PcaU from Acinetobacter sp. strain ADP1 governs expression of genes for protocatechuate degradation (pca genes) as a repressor or an activator depending on the levels of the inducer protocatechuate and of its own gene [12].
• A subfragment carrying the 5' end of argC and the region immediately upstream showed specific binding, in mobility shift assays, to purified AhrC, the repressor/activator of genes of arginine metabolism in Bacillus subtilis [13].

Biological context of c1546

• Repressor titration: a novel system for selection and stable maintenance of recombinant plasmids [14].
• MALDI-TOF mass analysis identified it with IscR, known to serve as a repressor of the iscRSUA gene expression under anaerobic condition as a [2Fe-2S]-bound form [15].
• Repressor binding to the fluorescein-labeled hairpin 20mer was compared with binding to a rhodamine-labeled 36 base-pair oligonucleotide bearing two inverted structural half-sites GNACT separated by an eight base-pair spacer containing none of the natural intervening sequence [16].
• Both purified CysK and CymR, the global repressor of cysteine metabolism, were required to observe the formation of a protein-DNA complex with the yrrT promoter region in gel-shift experiments [17].
• At the gadA promoter this regulatory element overlaps one of the binding sites of the repressor H-NS [18].

Associations of c1546 with chemical compounds

• Repressor cysteines are reactive toward these reagents in the order cysteine 140 greater than or equal to cysteine 107 greater than cysteine 281 [19].
• Mutations inactivating the Mnt repressor are recessive while those destroying operator recognition (Oc) are dominant in conferring tetracycline resistance on the host [20].
• Deletion of fdsR revealed a dual regulatory effect of FdsR on the fds operon by acting as transcriptional activator in the presence of formate or as repressor in the absence of formate [21].
• Glucose is a catabolite repressor of sporulation by C. perfringens [22].
• Among others, two major regulators, the repressor Mlc and the cyclic AMP-cyclic AMP receptor protein activator complex, have been identified [23].

Analytical, diagnostic and therapeutic context of c1546

• These sequences were examined by site-directed mutagenesis and found to be important in repressor binding and in the binding of a catabolite repressor [24].
• YefM, a dimeric protein with extensive secondary structure revealed by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy, is the primary repressor, whereas YoeB is a repression enhancer [25].

References