Disease relevance of ompF

• ChxR functions as a transcriptional regulator because it activated transcription of ompF and ompC when expressed in Escherichia coli [1].
• We have isolated a novel outer membrane protein (OMP)-encoding gene from Salmonella typhi (St), termed ompS1, using the ompF gene of Escherichia coli (Ec) as a heterologous probe [2].
• The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes [3].

High impact information on ompF

• Among such derivatives, we have isolated a temperature-sensitive conditional lethal mutant that accumulates the precursor of the maltose-binding protein in the cytoplasm, and also accumulates precursors of alkaline phosphatase, lambda receptor protein and the ompF gene gene product [4].
• DNase I protection studies showed that ChxR bound to sites in the ompF and ompC promoter proximal regions that overlap but were distinct from OmpR binding sites [1].
• The histidine kinase/response regulator system EnvZ/OmpR of Escherichia coli regulates transcription of the genes ompF and ompC, encoding two porins of the outer membrane [5].
• The OmpR protein of Escherichia coli binds to sites in the ompF promoter region in a hierarchical manner determined by its degree of phosphorylation [6].
• By using purified OmpR and various regions of the ompF promoter we show that phosphorylation causes binding of OmpR to a DNA region between the -40 to -100 region of the ompF promoter previously shown to be important for ompF expression [6].

Chemical compound and disease context of ompF

• External-pH-dependent expression of the maltose regulon and ompF gene in Escherichia coli is affected by the level of glycerol kinase, encoded by glpK [7].
• For the excretion of human beta-endorphin as a model protein, the beta-endorphin gene was fused to the C terminus of the E. coli ompF gene by using a linker containing the Factor Xa recognition site [8].
• Regulation of ompF porin expression by salicylate in Escherichia coli [9].

Biological context of ompF

• These results suggest that ompF and ompC expression is associated with other physiological regulating systems in addition to osmoregulation [10].
• Identification of the genes in multicopy plasmids affecting ompC and ompF expression in Escherichia coli [10].
• Using an E. coli genomic library in pUC19, we identified three clones whose products altered expression of ompC and ompF in response to medium osmolarity [10].
• OmpR acts as a positive as well as a negative regulator of ompF expression by binding to DNA sequences in the ompF promoter region [6].
• The ompF gene of E. coli BL21(DE3) was first knocked out by using the red operon of bacteriophage lambda to construct E. coli MBEL-BL101 [8].

Anatomical context of ompF

• These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment [11].
• The opening and closing of the ompF porin from Escherichia coli JF 701 was investigated by reconstituting the purified protein into planar bilayer membranes [12].
• The mutation also affected some outer membrane proteins, among which were the ompF protein and a protein which may be protein III, but had little effect on cytoplasmic membrane proteins [13].

Associations of ompF with chemical compounds

• A chimera gene consisting of the ompF promoter, the coding regions for the signal peptide and the NH2-terminal 11 amino acid residues of outer membrane OmpF protein, and the coding region for the major outer membrane lipoprotein devoid of the NH2-terminal 7 amino acid residues was constructed [14].
• Transcriptional and translational ompF fusions showed a sharp peak of expression under glucose limitation at D = 0.3 h-1, with lower amounts at lower and higher growth rates [15].
• Nicotinamide induced the expression of ompF when the osmo-remedial strains were grown under conditions of low osmolality [16].
• The cpxA mutant exhibited increased transcription of ompC and a very strong decrease in transcription of ompF under conditions in which acetyl phosphate levels were high [17].
• Expression of the ompF and ompC genes coding for major outer membrane proteins is osmoregulated by solutes, such as sucrose and NaCl, in the growth medium [18].

Analytical, diagnostic and therapeutic context of ompF

• Gel retardation assays and DNase I protection experiments showed that IHF binds to two sites in the ompF promoter region centered at positions -180 and -60 relative to the start of transcription [19].
• Molecular analysis by deletion and site-directed mutagenesis of the cis-acting upstream sequence involved in activation of the ompF promoter in Escherichia coli [20].

References