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Gene Review

ompF  -  outer membrane protein F

Escherichia coli CFT073

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Disease relevance of ompF


High impact information on ompF

  • Among such derivatives, we have isolated a temperature-sensitive conditional lethal mutant that accumulates the precursor of the maltose-binding protein in the cytoplasm, and also accumulates precursors of alkaline phosphatase, lambda receptor protein and the ompF gene gene product [4].
  • DNase I protection studies showed that ChxR bound to sites in the ompF and ompC promoter proximal regions that overlap but were distinct from OmpR binding sites [1].
  • The histidine kinase/response regulator system EnvZ/OmpR of Escherichia coli regulates transcription of the genes ompF and ompC, encoding two porins of the outer membrane [5].
  • The OmpR protein of Escherichia coli binds to sites in the ompF promoter region in a hierarchical manner determined by its degree of phosphorylation [6].
  • By using purified OmpR and various regions of the ompF promoter we show that phosphorylation causes binding of OmpR to a DNA region between the -40 to -100 region of the ompF promoter previously shown to be important for ompF expression [6].

Chemical compound and disease context of ompF


Biological context of ompF


Anatomical context of ompF

  • These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment [11].
  • The opening and closing of the ompF porin from Escherichia coli JF 701 was investigated by reconstituting the purified protein into planar bilayer membranes [12].
  • The mutation also affected some outer membrane proteins, among which were the ompF protein and a protein which may be protein III, but had little effect on cytoplasmic membrane proteins [13].

Associations of ompF with chemical compounds

  • A chimera gene consisting of the ompF promoter, the coding regions for the signal peptide and the NH2-terminal 11 amino acid residues of outer membrane OmpF protein, and the coding region for the major outer membrane lipoprotein devoid of the NH2-terminal 7 amino acid residues was constructed [14].
  • Transcriptional and translational ompF fusions showed a sharp peak of expression under glucose limitation at D = 0.3 h-1, with lower amounts at lower and higher growth rates [15].
  • Nicotinamide induced the expression of ompF when the osmo-remedial strains were grown under conditions of low osmolality [16].
  • The cpxA mutant exhibited increased transcription of ompC and a very strong decrease in transcription of ompF under conditions in which acetyl phosphate levels were high [17].
  • Expression of the ompF and ompC genes coding for major outer membrane proteins is osmoregulated by solutes, such as sucrose and NaCl, in the growth medium [18].

Analytical, diagnostic and therapeutic context of ompF


  1. ChxR is a transcriptional activator in Chlamydia. Koo, I.C., Walthers, D., Hefty, P.S., Kenney, L.J., Stephens, R.S. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  2. Isolation and characterization of ompS1, a novel Salmonella typhi outer membrane protein-encoding gene. Fernández-Mora, M., Oropeza, R., Puente, J.L., Calva, E. Gene (1995) [Pubmed]
  3. Specialized transducing bacteriophage lambda carrying the structural gene for a major outer membrane matrix protein of Escherichia coli K-12. Mutoh, N., Nagasawa, T., Mizushima, S. J. Bacteriol. (1981) [Pubmed]
  4. E. coli mutant pleiotropically defective in the export of secreted proteins. Oliver, D.B., Beckwith, J. Cell (1981) [Pubmed]
  5. K+ stimulates specifically the autokinase activity of purified and reconstituted EnvZ of Escherichia coli. Jung, K., Hamann, K., Revermann, A. J. Biol. Chem. (2001) [Pubmed]
  6. The OmpR protein of Escherichia coli binds to sites in the ompF promoter region in a hierarchical manner determined by its degree of phosphorylation. Rampersaud, A., Harlocker, S.L., Inouye, M. J. Biol. Chem. (1994) [Pubmed]
  7. External-pH-dependent expression of the maltose regulon and ompF gene in Escherichia coli is affected by the level of glycerol kinase, encoded by glpK. Chagneau, C., Heyde, M., Alonso, S., Portalier, R., Laloi, P. J. Bacteriol. (2001) [Pubmed]
  8. Excretion of human beta-endorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coli. Jeong, K.J., Lee, S.Y. Appl. Environ. Microbiol. (2002) [Pubmed]
  9. Regulation of ompF porin expression by salicylate in Escherichia coli. Rosner, J.L., Chai, T.J., Foulds, J. J. Bacteriol. (1991) [Pubmed]
  10. Identification of the genes in multicopy plasmids affecting ompC and ompF expression in Escherichia coli. Jin, T., Inouye, M. FEMS Microbiol. Lett. (1995) [Pubmed]
  11. Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system. Bowrin, V., Brissette, R., Inouye, M. J. Bacteriol. (1992) [Pubmed]
  12. Channel-closing activity of porins from Escherichia coli in bilayer lipid membranes. Xu, G.Z., Shi, B., McGroarty, E.J., Tien, H.T. Biochim. Biophys. Acta (1986) [Pubmed]
  13. ExpA: a conditional mutation affecting the expression of a group of exported proteins in Escherichia coli K-12. Dassa, E., Boquet, P.L. Mol. Gen. Genet. (1981) [Pubmed]
  14. Mechanism of localization of major outer membrane lipoprotein in Escherichia coli. Studies with the OmpF-lipoprotein hybrid protein. Yu, F., Furukawa, H., Nakamura, K., Mizushima, S. J. Biol. Chem. (1984) [Pubmed]
  15. Regulation of porin-mediated outer membrane permeability by nutrient limitation in Escherichia coli. Liu, X., Ferenci, T. J. Bacteriol. (1998) [Pubmed]
  16. Naturally-occurring, osmo-remedial variants of Escherichia coli. Kunin, C.M., Tong, H.H., Maher, W.E. J. Med. Microbiol. (1993) [Pubmed]
  17. The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC. Batchelor, E., Walthers, D., Kenney, L.J., Goulian, M. J. Bacteriol. (2005) [Pubmed]
  18. Molecular analysis of mutant ompR genes exhibiting different phenotypes as to osmoregulation of the ompF and ompC genes of Escherichia coli. Nara, F., Matsuyama, S., Mizuno, T., Mizushima, S. Mol. Gen. Genet. (1986) [Pubmed]
  19. In vitro interactions of integration host factor with the ompF promoter-regulatory region of Escherichia coli. Ramani, N., Huang, L., Freundlich, M. Mol. Gen. Genet. (1992) [Pubmed]
  20. Molecular analysis by deletion and site-directed mutagenesis of the cis-acting upstream sequence involved in activation of the ompF promoter in Escherichia coli. Kato, M., Aiba, H., Mizuno, T. J. Biochem. (1989) [Pubmed]
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