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POLQ  -  polymerase (DNA directed), theta

Homo sapiens

Synonyms: DNA polymerase eta, DNA polymerase theta, POLH, PRO0327
 
 
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Disease relevance of POLQ

 

High impact information on POLQ

  • The translesion DNA polymerase theta plays a dominant role in immunoglobulin gene somatic hypermutation [6].
  • POLQ has three sequence insertions between conserved motifs in its catalytic site [7].
  • The proteins encoded by the XPA, -B, -C, -D, -F, and -G genes are required for nucleotide excision repair, and the XPV gene encodes DNA polymerase eta, which carries out translesion DNA synthesis [8].
  • The role of DNA polymerase eta in translesion synthesis past platinum-DNA adducts in human fibroblasts [9].
  • Biochemical basis of genotoxicity of heterocyclic arylamine food mutagens: Human DNA polymerase eta selectively produces a two-base deletion in copying the N2-guanyl adduct of 2-amino-3-methylimidazo[4,5-f]quinoline but not the C8 adduct at the NarI G3 site [10].
 

Chemical compound and disease context of POLQ

  • PCR amplification of the highly conserved baculovirus genes late expression factor 8 (lef-8), late expression factor 9 (lef-9) and polyhedrin/granulin (polh/gran) combined with molecular phylogenetic analyses provide a powerful tool to identify lepidopteran-specific baculoviruses and to study their diversity [11].
 

Biological context of POLQ

  • These results are consistent with a requirement for functional DNA polymerase eta in the replication of a double-stranded plasmid containing either a (6-4) photoproduct or a CPD, on the leading but not the lagging strand [12].
  • UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK [13].
 

Anatomical context of POLQ

 

Associations of POLQ with chemical compounds

  • Error-prone translesion synthesis by human DNA polymerase eta on DNA-containing deoxyadenosine adducts of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [16].
  • Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta [17].
  • The N2-ethylguanine and the O6-ethyl- and O6-methylguanine lesions in DNA: contrasting responses from the "bypass" DNA polymerase eta and the replicative DNA polymerase alpha [18].
  • Translesion synthesis by human DNA polymerase eta across oxidative products of guanine [19].
  • Thymine-thymine CPDs, and probably also CPDs containing cytosine, are replicated in vivo in a largely accurate manner by a DNA polymerase eta (Pol eta) dependent process [20].
 

Other interactions of POLQ

 

Analytical, diagnostic and therapeutic context of POLQ

  • Twenty-four hours after infection, the DNA polymerase eta-EGFP fusion protein was detected by Western blot analysis, demonstrating successful transduction by the adenoviral vector [4].

References

  1. Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol. Takata, K., Shimizu, T., Iwai, S., Wood, R.D. J. Biol. Chem. (2006) [Pubmed]
  2. POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in human cells. Seki, M., Marini, F., Wood, R.D. Nucleic Acids Res. (2003) [Pubmed]
  3. DNA damage responses protect xeroderma pigmentosum variant from UVC-induced clastogenesis. Cordeiro-Stone, M., Frank, A., Bryant, M., Oguejiofor, I., Hatch, S.B., McDaniel, L.D., Kaufmann, W.K. Carcinogenesis (2002) [Pubmed]
  4. Adenovirus mediated transduction of the human DNA polymerase eta cDNA. Lima-Bessa, K.M., Chiganças, V., Stary, A., Kannouche, P., Sarasin, A., Armelini, M.G., de Fátima Jacysyn, J., Amarante-Mendes, G.P., Cordeiro-Stone, M., Cleaver, J.E., Menck, C.F. DNA Repair (Amst.) (2006) [Pubmed]
  5. Pathogenesis of AIDS lymphoma: role of oncogenic viruses and B cell activation-associated molecular lesions. Epeldegui, M., Widney, D.P., Martínez-Maza, O. Current opinion in oncology. (2006) [Pubmed]
  6. The translesion DNA polymerase theta plays a dominant role in immunoglobulin gene somatic hypermutation. Zan, H., Shima, N., Xu, Z., Al-Qahtani, A., Evinger Iii, A.J., Zhong, Y., Schimenti, J.C., Casali, P. EMBO J. (2005) [Pubmed]
  7. High-efficiency bypass of DNA damage by human DNA polymerase Q. Seki, M., Masutani, C., Yang, L.W., Schuffert, A., Iwai, S., Bahar, I., Wood, R.D. EMBO J. (2004) [Pubmed]
  8. Xeroderma pigmentosum complementation group E protein (XPE/DDB2): purification of various complexes of XPE and analyses of their damaged DNA binding and putative DNA repair properties. Kulaksiz, G., Reardon, J.T., Sancar, A. Mol. Cell. Biol. (2005) [Pubmed]
  9. The role of DNA polymerase eta in translesion synthesis past platinum-DNA adducts in human fibroblasts. Bassett, E., King, N.M., Bryant, M.F., Hector, S., Pendyala, L., Chaney, S.G., Cordeiro-Stone, M. Cancer Res. (2004) [Pubmed]
  10. Biochemical basis of genotoxicity of heterocyclic arylamine food mutagens: Human DNA polymerase eta selectively produces a two-base deletion in copying the N2-guanyl adduct of 2-amino-3-methylimidazo[4,5-f]quinoline but not the C8 adduct at the NarI G3 site. Choi, J.Y., Stover, J.S., Angel, K.C., Chowdhury, G., Rizzo, C.J., Guengerich, F.P. J. Biol. Chem. (2006) [Pubmed]
  11. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Jehle, J.A., Lange, M., Wang, H., Hu, Z., Wang, Y., Hauschild, R. Virology (2006) [Pubmed]
  12. A single (6-4) photoproduct inhibits plasmid DNA replication in xeroderma pigmentosum variant cell extracts. Yao, J., Dixon, K., Carty, M.P. Environ. Mol. Mutagen. (2001) [Pubmed]
  13. UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK. Cruet-Hennequart, S., Coyne, S., Glynn, M.T., Oakley, G.G., Carty, M.P. DNA Repair (Amst.) (2006) [Pubmed]
  14. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Kawamura, K., Bahar, R., Seimiya, M., Chiyo, M., Wada, A., Okada, S., Hatano, M., Tokuhisa, T., Kimura, H., Watanabe, S., Honda, I., Sakiyama, S., Tagawa, M., O-Wang, J. Int. J. Cancer (2004) [Pubmed]
  15. Deficiency of the Caenorhabditis elegans DNA polymerase eta homologue increases sensitivity to UV radiation during germ-line development. Ohkumo, T., Masutani, C., Eki, T., Hanaoka, F. Cell Struct. Funct. (2006) [Pubmed]
  16. Error-prone translesion synthesis by human DNA polymerase eta on DNA-containing deoxyadenosine adducts of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene. Chiapperino, D., Cai, M., Sayer, J.M., Yagi, H., Kroth, H., Masutani, C., Hanaoka, F., Jerina, D.M., Cheh, A.M. J. Biol. Chem. (2005) [Pubmed]
  17. Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta. Vaisman, A., Masutani, C., Hanaoka, F., Chaney, S.G. Biochemistry (2000) [Pubmed]
  18. The N2-ethylguanine and the O6-ethyl- and O6-methylguanine lesions in DNA: contrasting responses from the "bypass" DNA polymerase eta and the replicative DNA polymerase alpha. Perrino, F.W., Blans, P., Harvey, S., Gelhaus, S.L., McGrath, C., Akman, S.A., Jenkins, G.S., LaCourse, W.R., Fishbein, J.C. Chem. Res. Toxicol. (2003) [Pubmed]
  19. Translesion synthesis by human DNA polymerase eta across oxidative products of guanine. Kino, K., Ito, N., Sugasawa, K., Sugiyama, H., Hanaoka, F. Nucleic acids symposium series (2004) (2004) [Pubmed]
  20. The role of DNA polymerase eta in UV mutational spectra. Choi, J.H., Pfeifer, G.P. DNA Repair (Amst.) (2005) [Pubmed]
  21. DNA polymerase theta purified from human cells is a high-fidelity enzyme. Maga, G., Shevelev, I., Ramadan, K., Spadari, S., Hübscher, U. J. Mol. Biol. (2002) [Pubmed]
 
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