Disease relevance of CDC42EP1

• Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility [1].
• Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization [2].
• Ninety-one percent of NCCTG gliomas with 1p/19q deletion and 12% without 1p/19q deletion had CEP1/19p12 fusion (P < 0.001, chi(2) test) [3].
• Among NCCTG patients, CEP1/19p12 fusion prevalence was 55%, 47%, and 0% among the oligodendrogliomas, mixed oligoastrocytomas, and astrocytomas, respectively [3].

High impact information on CDC42EP1

• Here we show that epithelial cells expressing constitutively active or kinase-dead mutants of the Rac/Cdc42 effector Pak1 fail to undergo growth arrest upon wound closure [4].
• In Cos-7 cells, wild-type MSE55 localized at membrane ruffles and increased membrane actin polymerization, whereas expression of the MSE55 CRIB mutant showed fewer membrane ruffles [5].
• MSE55, a Cdc42 effector protein, induces long cellular extensions in fibroblasts [5].
• Using glutathione S-transferase-capture experiments, we show that MSE55 binds to Cdc42 in a GTP-dependent manner [5].
• In contrast to these results, MSE55 induced the formation of long, actin-based protrusions in NIH 3T3 cells as detected by immunofluorescence and live-cell video microscopy [5].

Biological context of CDC42EP1

• Interphase fusion of CEP1 and 19p12 probes detected the t(1;19) [3].
• CEP1/19p12 fusion was observed in all metaphases and 74% of interphase nuclei [3].
• Stimulation of M3 muscarinic receptors induces phosphorylation of the Cdc42 effector activated Cdc42Hs-associated kinase-1 via a Fyn tyrosine kinase signaling pathway [6].
• We also searched for the Cdc42(V12) target but overexpression of the Cdc42 effector WASP did not mimic the inhibition of exocytosis observed in cells transfected with the activated GTPase [7].

Anatomical context of CDC42EP1

• On this basis, we have designated this novel protein MSE55, for marrow stromal/endothelial cell protein with a molecular mass of 55,000 daltons [8].
• cDNA cloning and molecular characterization of MSE55, a novel human serum constituent protein that displays bone marrow stromal/endothelial cell-specific expression [8].
• Also, expression of the verprolin-cofilin-acidic domain of N-WASP, a downstream Cdc42 effector, in ECs completely interfered with vacuole and lumen formation [9].
• Human fertility protein PUMILIO2 interacts in vitro with testis mRNA encoding Cdc42 effector 3 (CEP3) [10].
• A study by Fukata et al. in the June 28th issue of Cell now shows that the Rac1/Cdc42 effector IQGAP1 captures microtubules by binding to CLIP170 [11].

Associations of CDC42EP1 with chemical compounds

• A drastic reduction of chemoattractant-induced inositol-1,4,5-trisphosphate formation and calcium mobilization was observed, corroborating previous in vitro study results identifying PLCbeta2 as a Rac/Cdc42 effector [12].

Other interactions of CDC42EP1

• CIP4, like WASP, is a Cdc42 effector protein involved in cytoskeletal organization [13].
• SNX9 as an adaptor for linking synaptojanin-1 to the Cdc42 effector ACK1 [14].
• Cdc42-driven migration and p130(Cas) phosphorylation were dependent on the Cdc42 effector Ack1 (activated Cdc42-associated kinase) [15].

Analytical, diagnostic and therapeutic context of CDC42EP1

• During the treadmill test, the mean exercise time to submaximal exertion (Borg 5/10), exertion ratings and respiratory rate did not differ between pacing modes despite a significantly lower ventricular rate in the VVI,R mode [16].

References