Disease relevance of Igh-VS107

• Retention of an idiotypic determinant in a human B-cell lymphoma undergoing immunoglobulin variable-region mutation [1].
• Restricted immunoglobulin variable region (Ig V) gene expression accompanies secondary rearrangements of light chain Ig V genes in mouse plasmacytomas [2].
• Biased utilization of immunoglobulin variable region heavy- and light-chain genes by the malignant CD5- B lymphocytes from patients with Burkitt's lymphoma [3].
• A technique, Replacement PCR Mutagenesis, was developed to replace one immunoglobulin variable region (V) in a M13 phage cassette with a different, homologous V [4].

High impact information on Igh-VS107

• Activation-induced cytidine deaminase (AID), which is specific to B lymphocytes, is required for class switch recombination (CSR)--a process mediating isotype switching of immunoglobulin--and somatic hypermutation--the introduction of many point mutations into the immunoglobulin variable region genes [5].
• Immunoglobulin variable region (IgV) genes are diversified after gene rearrangement by hypermutation [6].
• Targeted hypermutation of immunoglobulin variable region genes occurs in B cells during an immune response, and gives rise to families of related mutant antibodies which are then selected for their binding affinity to the immunizing antigen [7].
• Immunoglobulin variable-region (V) genes, it is now recognized, do not encode specific receptors for T lymphocytes [8].
• The humoral immune response of the mouse to certain antigens is characterized by the dominant expression of a single or limited number of related, immunoglobulin variable region (V) structures by antibody-secreting lymphocytes [9].

Biological context of Igh-VS107

• Somatic point mutations are usually found in the coding and flanking regions of functionally and aberrantly rearranged immunoglobulin variable region gene segments [10].
• Sequence analysis of three of the VH7183-containing restriction fragments indicate that all are pseudogenes which contain interruptions at either the 5' and/or 3' ends of the VH coding region [11].
• To better understand the molecular basis of the antibody response, the heavy- and light-chain immunoglobulin variable region (VH and VL, respectively) sequences of seven monoclonal antibodies (MAbs) to GXM were determined [12].
• Many immunoglobulin variable region (IgV) genes are present in the vertebrate genome and provide a basis for antibody diversity [13].
• The Igh locus is controlled by cis-acting elements, including Emu and the 3' IgH regulatory region which flank the C region genes within the well-studied 3' part of the locus [14].

Anatomical context of Igh-VS107

• The molecular analysis of 12 such hybridomas revealed that all IgH loci were rearranged into DJH or productive or nonproductive VHDJH complexes [15].
• Cell mixing experiments with genetically marked cells indicated that each colony is derived from a single progenitor cell not yet committed to the expression of either IgH locus [15].
• Evidence for the presence of idiotype-bearing regulatory T cells in which idiotype expression does not show linkage to either IgH alleles or the MHC [16].
• Immunoglobulin variable region gene sequencing, and 5-bromo-2'-deoxyuridine pulse-chase studies indicate that long-lived splenic plasma cells are a mixture of cells derived from the extrafollicular and germinal center responses and cells derived from virgin and memory B cells [17].
• Immunoglobulin variable region hypermutation in hybrids derived from a pre-B- and a myeloma cell line [18].

Associations of Igh-VS107 with chemical compounds

• Restricted immunoglobulin variable region gene usage by normal Ly-1 (CD5+) B cells that recognize phosphatidyl choline [19].
• The NH2-terminal 110 residues show clear homology to the T-cell receptor and immunoglobulin variable region sequences [20].

Other interactions of Igh-VS107

• In order to define the genetic basis and investigate the molecular mechanisms resulting in pathogenic anti-mouse erythrocyte autoantibody production, the pattern of immunoglobulin variable region gene use has been studied [21].
• Comparison of the alpha cDNA with genomic sequence data indicates that there are five exons encoding Lyt-2: a fused leader/immunoglobulin variable region-like exon, a spacer region exon, a transmembrane exon, and two cytoplasmic exons [22].
• Searches of protein data bases revealed that Ly-3 is a member of the immunoglobulin superfamily with significant homology to Ly-2, immunoglobulin variable region kappa and lambda light chains, and the beta chain of the T-cell receptor [23].
• Ceacam11 cDNA encodes 303 amino acids with a possible signal peptide and two immunoglobulin variable region-like domains [24].

Analytical, diagnostic and therapeutic context of Igh-VS107

• We report a novel approach to the generation of monoclonal antibodies based on the molecular cloning and expression of immunoglobulin variable region cDNAs generated from single rabbit or murine lymphocytes that were selected for the production of specific antibodies [25].
• Cloning and sequencing of immunoglobulin variable-region genes using degenerate oligodeoxyribonucleotides and polymerase chain reaction [26].
• Upon adoptive transfer, OVA-specific PC from bone marrow, but not memory B cells, conferred specific and long-lasting antibody titers to antigen-free IgH syngeneic recipients [27].

References