Disease relevance of SYF2

• The tests for antibodies to p29 and myeloperoxidase were 99.9 and 99.5% specific for pauci-immune necrotizing and crescentic glomerulonephritis, respectively [1].
• Cloned antibodies to hepatitis D antigen detected by ELISA and/or immunofluorescence staining recognized the two major specific native and denatured polypeptides, p27 and p29, in Western blot analysis [2].
• The prognostic value of the monoclonal antibody (D5) detected protein, p29, in primary colorectal carcinoma [3].
• In addition, p29 protein was induced by heat shock in the estrogen receptor negative MDA-MB-231 human breast tumor cell line [4].
• Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer [4].

High impact information on SYF2

• Antisera have been prepared against the separated glycoprotein chains (p29 and p34) of HLA-DR antigens (p29,34) isolated from membranes of the B lymphoblastoid cell line JY (DRw4,6) [5].
• Anti-p29 and anti-p34 specifically immunoprecipitated the precursors to p29 and p34, respectively, from the cell-free translation products of a rabbit reticulocyte lysate system supplemented with polyadenylic acid-containing messenger RNA (mRNA) isolated from JY cells [5].
• Utilizing a multielectrode array to record spontaneous and visually evoked activity of cortical neurons in area 17, we investigate the relationship between long-range correlated spontaneous activity and functional ocular dominance columns during early ferret postnatal development (P24-P29) [6].
• Nucleotide sequence analysis indicated that p29 and p40 were encoded by a single large open reading frame [7].
• Examination of the p29 amino acid sequence revealed similarity to the Potyvirus-encoded cysteine-type proteinase HC-Pro [7].

Biological context of SYF2

• The coding regions for p29 and p40 were mapped to nonoverlapping portions of the 5'- and 3'-terminal domains of the open reading frame, respectively [7].
• The p29 cleavage product was also detected during T-cell receptor-mediated apoptosis in peripheral blood lymphocytes from normal donors [8].
• Gene expression by a viral-like double-stranded RNA genetic element associated with reduced virulence (hypovirulence) of the chestnut blight fungus was recently shown to involve an autoproteolytic event which resulted in the release of an encoded protease, designated p29, from a polyprotein during translation [9].
• In normal tissue p29 was present throughout the ectocervix during the menstrual cycle and virtually absent from the endocervix [10].

Anatomical context of SYF2

• The transient expression of HA-tagged p29 in HeLa cells localized in the nucleus [11].
• Multiple tissue Northern blot analysis showed that p29 was abundantly expressed in the heart, skeletal muscle, and kidney relative to other tissues [11].
• With the exception of the previously identified p29 protein, the pattern of detectably altered protein synthesis during glucocorticoid treatment was identical in both cell lines [12].
• This p29 cleaved fragment was detected in Jurkat cells induced to apoptose by anti-Fas antibody, staurosporin, or VP-16 [8].
• The HLA-linked B cell alloantigen (p29,34) is composed of two subunits of 29,000 (p29) and 34,000 (p34) molecular weight [13].

Associations of SYF2 with chemical compounds

• In sodium dodecyl sulfate at 21 degrees, p29 and p34 remained noncovalently associated and retained immunologic activity; subunit dissociation at higher temperatures correlated with loss of immunologic activity [13].
• A 29-kDa serine protease (p29 or proteinase 3) and myeloperoxidase are the two best characterized antigens recognized by ANCA [1].
• Seventy-three primary human breast cancers were analyzed to assess the presence of estrogen and progesterone receptors, the p29 protein, and the total cathepsin D status [14].
• Tumours expressing ER (by either steroid binding assay or EIA) had higher levels of p29 than those which did not express the receptor (P < 0.0001) [15].
• Moreover, tumours co-expressing ER, PR and p29 appeared to have higher levels of cathepsin D than those which were negative for at least one protein (P < 0.0001) [15].

Other interactions of SYF2

• p29, a novel GCIP-interacting protein, localizes in the nucleus [11].

Analytical, diagnostic and therapeutic context of SYF2

• One novel gene, p29, was demonstrated to associate with GCIP in the yeast two-hybrid method and in vitro GST pull-down assay [11].
• Using a monoclonal antibody raised against an oestrogen receptor-related protein, p29, and an indirect immunoperoxidase technique to stain human tissue, the presence of the antigen was investigated in normal, dysplastic and malignant tissue of the uterine cervix [10].
• Identical hsp27 and p29 immunostaining intensity, distribution, and percentage of stained cells was demonstrated by immunocytochemistry [4].
• From two-dimensional gel electrophoresis it was determined that p116 and p29 had a pl of 4 [16].
• An immunoradiometric assay was used to determine the presence of p29 protein in 68 breast cancer cyTOSOLS [17].

References