The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Dctn1  -  dynactin 1

Rattus norvegicus

Synonyms: 150 kDa dynein-associated polypeptide, DAP-150, DP-150, Dynactin subunit 1, p150-glued
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Dctn1


High impact information on Dctn1

  • Dynein and dynactin complex components concentrate on the nuclear envelope prior to any changes in nuclear envelope organization [2].
  • Cytoplasmic dynein is the major minus end-directed microtubule motor in animal cells, and associates with many of its cargoes in conjunction with the dynactin complex [3].
  • Motility of apoptotic membranes is restored by recruitment of intact cytoplasmic dynein and dynactin from control cytosol, or from apoptotic cytosol supplemented with purified cytoplasmic dynein--dynactin, demonstrating the dynamic nature of the association of cytoplasmic dynein and dynactin with their membrane cargo [3].
  • On the basis of these results, we conclude that cytoplasmic dynein and dynactin are essential for the transport of microtubules from the centrosome into the axon [4].
  • Evidence is provided for a dynactin-associated kinase required for dynein-mediated vesicle transport [5].

Biological context of Dctn1


Anatomical context of Dctn1


Associations of Dctn1 with chemical compounds

  • In order to clone the p24 subunit, the components of purified dynactin were resolved by SDS polyacrylamide gel electrophoresis [12].
  • This polypeptide co-sediments with dynactin on sucrose density gradients and it also co-immunoprecipitates with dynactin, but not with kinesin or cytoplasmic dynein [12].

Physical interactions of Dctn1


Other interactions of Dctn1

  • As compared with early vesicles, late vesicles had reduced association with receptor, Rab4, and kinesin I but were highly associated with dynein, Rab7, dynactin, and KIF3A [14].
  • Secretory granules of non-polarized chromaffin cells of the bovine adrenal medulla, that are assumed to underlie microtubular plus end targeting from the Golgi apparatus to the cell periphery, were not found to be associated with dynein or dynactin [15].
  • We investigated whether the motor protein cytoplasmic dynein and dynactin, a protein complex thought to link dynein with vesicles, are present in rat renal collecting ducts and associated with aquaporin-2 (AQP2)-bearing vesicles [16].

Analytical, diagnostic and therapeutic context of Dctn1


  1. Human DCTN1: genomic structure and evaluation as a candidate for Alström syndrome. Collin, G.B., Nishina, P.M., Marshall, J.D., Naggert, J.K. Genomics (1998) [Pubmed]
  2. Cytoplasmic dynein as a facilitator of nuclear envelope breakdown. Salina, D., Bodoor, K., Eckley, D.M., Schroer, T.A., Rattner, J.B., Burke, B. Cell (2002) [Pubmed]
  3. Apoptotic cleavage of cytoplasmic dynein intermediate chain and p150(Glued) stops dynein-dependent membrane motility. Lane, J.D., Vergnolle, M.A., Woodman, P.G., Allan, V.J. J. Cell Biol. (2001) [Pubmed]
  4. Cytoplasmic dynein and dynactin are required for the transport of microtubules into the axon. Ahmad, F.J., Echeverri, C.J., Vallee, R.B., Baas, P.W. J. Cell Biol. (1998) [Pubmed]
  5. Molecular requirements for bi-directional movement of phagosomes along microtubules. Blocker, A., Severin, F.F., Burkhardt, J.K., Bingham, J.B., Yu, H., Olivo, J.C., Schroer, T.A., Hyman, A.A., Griffiths, G. J. Cell Biol. (1997) [Pubmed]
  6. Regulation of intracellular trafficking of huntingtin-associated protein-1 is critical for TrkA protein levels and neurite outgrowth. Rong, J., McGuire, J.R., Fang, Z.H., Sheng, G., Shin, J.Y., Li, S.H., Li, X.J. J. Neurosci. (2006) [Pubmed]
  7. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis. Echeverri, C.J., Paschal, B.M., Vaughan, K.T., Vallee, R.B. J. Cell Biol. (1996) [Pubmed]
  8. The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1). Waterman-Storer, C.M., Karki, S., Holzbaur, E.L. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  9. Functional analysis of dynactin and cytoplasmic dynein in slow axonal transport. Dillman, J.F., Dabney, L.P., Karki, S., Paschal, B.M., Holzbaur, E.L., Pfister, K.K. J. Neurosci. (1996) [Pubmed]
  10. Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued. Vaughan, K.T., Vallee, R.B. J. Cell Biol. (1995) [Pubmed]
  11. Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued subunit of dynactin. Engelender, S., Sharp, A.H., Colomer, V., Tokito, M.K., Lanahan, A., Worley, P., Holzbaur, E.L., Ross, C.A. Hum. Mol. Genet. (1997) [Pubmed]
  12. Identification and molecular characterization of the p24 dynactin light chain. Pfister, K.K., Benashski, S.E., Dillman, J.F., Patel-King, R.S., King, S.M. Cell Motil. Cytoskeleton (1998) [Pubmed]
  13. Cytoplasmic dynein intermediate chain phosphorylation regulates binding to dynactin. Vaughan, P.S., Leszyk, J.D., Vaughan, K.T. J. Biol. Chem. (2001) [Pubmed]
  14. Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for Dynein and Kinesin. Bananis, E., Nath, S., Gordon, K., Satir, P., Stockert, R.J., Murray, J.W., Wolkoff, A.W. Mol. Biol. Cell (2004) [Pubmed]
  15. Cytoplasmic dynein and dynactin as likely candidates for microtubule-dependent apical targeting of pancreatic zymogen granules. Kraemer, J., Schmitz, F., Drenckhahn, D. Eur. J. Cell Biol. (1999) [Pubmed]
  16. Dynein and dynactin colocalize with AQP2 water channels in intracellular vesicles from kidney collecting duct. Marples, D., Schroer, T.A., Ahrens, N., Taylor, A., Knepper, M.A., Nielsen, S. Am. J. Physiol. (1998) [Pubmed]
  17. Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex. Karki, S., Holzbaur, E.L. J. Biol. Chem. (1995) [Pubmed]
  18. The interaction of neurofilaments with the microtubule motor cytoplasmic dynein. Wagner, O.I., Ascaño, J., Tokito, M., Leterrier, J.F., Janmey, P.A., Holzbaur, E.L. Mol. Biol. Cell (2004) [Pubmed]
WikiGenes - Universities