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Gene Review

GSPT1  -  G1 to S phase transition 1

Homo sapiens

Synonyms: 551G9.2, ERF3A, ETF3A, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, ...
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Disease relevance of GSPT1


High impact information on GSPT1


Chemical compound and disease context of GSPT1


Biological context of GSPT1

  • The mouse GSPT1 was expressed in a proliferation-dependent manner in Swiss 3T3 cells, whereas the expression of GSPT2 was constant during the cell-cycle progression [8].
  • Here we propose that GSPT2 originated through retrotransposition of processed GSPT1 transcript into the genome [9].
  • Additionally, processed GSPT1 interacts biochemically with IAPs and could promote caspase activation, IAP ubiquitination and apoptosis [10].
  • The aim of this study was to evaluate eRF3/GSPT1 gene as a potential genetic susceptibility associated locus for gastric cancer, analysing a stable GGC expansion in exon 1 encoding a polyglycine tract in the N-terminal domain of the protein [1].
  • No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1 [2].

Anatomical context of GSPT1


Associations of GSPT1 with chemical compounds


Physical interactions of GSPT1

  • We identify and characterize the human translation termination factor eRF3/GSPT1 as an interacting partner of RNase L [17].
  • Search for other GSPT-binding proteins in yeast two-hybrid screening system resulted in the identification of a cDNA encoding polyadenylate-binding protein (PABP), whose amino terminus is associating with the poly(A) tail of mRNAs presumably for their stabilization [18].

Regulatory relationships of GSPT1

  • By measuring the readthrough at a premature nonsense codon in a reporter mRNA, we found that eRF3a silencing induced an important increase in readthrough whereas eRF3b silencing had no significant effect [19].
  • Finally, our data indicate that the expression level of eRF3a controls the formation of the termination complex by modulating eRF1 protein stability [19].
  • Unregulated expression of the transcription factor E2F promotes the G1-to-S phase transition in cultured mammalian cells [20].

Other interactions of GSPT1

  • Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels [2].
  • A novel role of the mammalian GSPT/eRF3 associating with poly(A)-binding protein in Cap/Poly(A)-dependent translation [12].
  • This domain recruits a series of translation factors including poly(A)-interacting proteins (Paip1 and Paip2) and release factor 3 (RF3/GSPT) to the initiation complex on mRNA [21].
  • Instead, we find that the activation of cyclin E-associated kinase that normally accompanies the G1-to-S phase transition is inhibited [22].
  • Cyclins E and A in association with Cdk2 have been shown to play a role in the G1-to-S phase transition in mammalian cells [22].

Analytical, diagnostic and therapeutic context of GSPT1


  1. Polyglycine expansions in eRF3/GSPT1 are associated with gastric cancer susceptibility. Brito, M., Malta-Vacas, J., Carmona, B., Aires, C., Costa, P., Martins, A.P., Ramos, S., Conde, A.R., Monteiro, C. Carcinogenesis (2005) [Pubmed]
  2. Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. Malta-Vacas, J., Aires, C., Costa, P., Conde, A.R., Ramos, S., Martins, A.P., Monteiro, C., Brito, M. J. Clin. Pathol. (2005) [Pubmed]
  3. Mapping of the human GSPT1 gene, a human homolog of the yeast GST1 gene, to chromosomal band 16p13.1. Ozawa, K., Murakami, Y., Eki, T., Yokoyama, K., Soeda, E., Hoshino, S., Ui, M., Hanaoka, F. Somat. Cell Mol. Genet. (1992) [Pubmed]
  4. Germ line polymorphisms of p53 and CYP1A1 genes involved in human lung cancer. Kawajiri, K., Nakachi, K., Imai, K., Watanabe, J., Hayashi, S. Carcinogenesis (1993) [Pubmed]
  5. Human cytomegalovirus UL69 protein induces cells to accumulate in G1 phase of the cell cycle. Lu, M., Shenk, T. J. Virol. (1999) [Pubmed]
  6. CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Feng, H., Zhong, W., Punkosdy, G., Gu, S., Zhou, L., Seabolt, E.K., Kipreos, E.T. Nat. Cell Biol. (1999) [Pubmed]
  7. A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells. Hoshino, S., Miyazawa, H., Enomoto, T., Hanaoka, F., Kikuchi, Y., Kikuchi, A., Ui, M. EMBO J. (1989) [Pubmed]
  8. Molecular cloning of a novel member of the eukaryotic polypeptide chain-releasing factors (eRF). Its identification as eRF3 interacting with eRF1. Hoshino, S., Imai, M., Mizutani, M., Kikuchi, Y., Hanaoka, F., Ui, M., Katada, T. J. Biol. Chem. (1998) [Pubmed]
  9. Evolution of translation termination factor eRF3: is GSPT2 generated by retrotransposition of GSPT1's mRNA? Zhouravleva, G., Schepachev, V., Petrova, A., Tarasov, O., Inge-Vechtomov, S. IUBMB Life (2006) [Pubmed]
  10. The polypeptide chain-releasing factor GSPT1/eRF3 is proteolytically processed into an IAP-binding protein. Hegde, R., Srinivasula, S.M., Datta, P., Madesh, M., Wassell, R., Zhang, Z., Cheong, N., Nejmeh, J., Fernandes-Alnemri, T., Hoshino, S., Alnemri, E.S. J. Biol. Chem. (2003) [Pubmed]
  11. Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation. Xu, M., Sheppard, K.A., Peng, C.Y., Yee, A.S., Piwnica-Worms, H. Mol. Cell. Biol. (1994) [Pubmed]
  12. A novel role of the mammalian GSPT/eRF3 associating with poly(A)-binding protein in Cap/Poly(A)-dependent translation. Uchida, N., Hoshino, S., Imataka, H., Sonenberg, N., Katada, T. J. Biol. Chem. (2002) [Pubmed]
  13. NF-kappaB function in growth control: regulation of cyclin D1 expression and G0/G1-to-S-phase transition. Hinz, M., Krappmann, D., Eichten, A., Heder, A., Scheidereit, C., Strauss, M. Mol. Cell. Biol. (1999) [Pubmed]
  14. A-myb is expressed in bovine vascular smooth muscle cells during the late G1-to-S phase transition and cooperates with c-myc to mediate progression to S phase. Marhamati, D.J., Bellas, R.E., Arsura, M., Kypreos, K.E., Sonenshein, G.E. Mol. Cell. Biol. (1997) [Pubmed]
  15. The Bcr-Abl tyrosine kinase activates mitogenic signaling pathways and stimulates G1-to-S phase transition in hematopoietic cells. Cortez, D., Reuther, G., Pendergast, A.M. Oncogene (1997) [Pubmed]
  16. Cell cycle regulatory proteins are targets for induced differentiation of transformed cells: Molecular and clinical studies employing hybrid polar compounds. Marks, P.A., Richon, V.M., Rifkind, R.A. Int. J. Hematol. (1996) [Pubmed]
  17. A newly discovered function for RNase L in regulating translation termination. Le Roy, F., Salehzada, T., Bisbal, C., Dougherty, J.P., Peltz, S.W. Nat. Struct. Mol. Biol. (2005) [Pubmed]
  18. The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein. Hoshino, S., Imai, M., Kobayashi, T., Uchida, N., Katada, T. J. Biol. Chem. (1999) [Pubmed]
  19. Involvement of human release factors eRF3a and eRF3b in translation termination and regulation of the termination complex formation. Chauvin, C., Salhi, S., Le Goff, C., Viranaicken, W., Diop, D., Jean-Jean, O. Mol. Cell. Biol. (2005) [Pubmed]
  20. Expression of dominant-negative mutant DP-1 blocks cell cycle progression in G1. Wu, C.L., Classon, M., Dyson, N., Harlow, E. Mol. Cell. Biol. (1996) [Pubmed]
  21. Solution structure of the C-terminal domain from poly(A)-binding protein in Trypanosoma cruzi: a vegetal PABC domain. Siddiqui, N., Kozlov, G., D'Orso, I., Trempe, J.F., Gehring, K. Protein Sci. (2003) [Pubmed]
  22. A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells. Slingerland, J.M., Hengst, L., Pan, C.H., Alexander, D., Stampfer, M.R., Reed, S.I. Mol. Cell. Biol. (1994) [Pubmed]
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