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Gene Review

PABPC1  -  poly(A) binding protein, cytoplasmic 1

Homo sapiens

Synonyms: PAB1, PABP, PABP-1, PABP1, PABPC2, ...
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Disease relevance of PABPC1


High impact information on PABPC1


Biological context of PABPC1

  • Here, we show that PABPC1, like the major nuclear poly(A) binding protein PABPN1, associates with nuclear pre-mRNAs that are polyadenylated and intron containing [9].
  • Our findings demonstrate that PABPC1 associates with polyadenylated transcripts earlier in mammalian mRNA biogenesis than previously thought and offer insights into the mechanism by which PABPC1 is recruited to newly synthesized poly(A) [9].
  • Deletion analysis identified a 29 amino acid sequence in the new N-terminal region as the PABP-binding site [10].
  • The protein fragment contains a protein domain, PABC [for poly(A)-binding protein C-terminal domain], which is also found associated with the HECT family of ubiquitin ligases [11].
  • Sequence profile searches and comparative protein structure modeling identified a small ORF from the Arabidopsis thaliana genome that encodes a structurally similar but distantly related PABP/HYD domain [12].

Anatomical context of PABPC1

  • In contrast, PABP is constitutively expressed in both resting and activated T cells. iPABP mRNA was also expressed at much higher levels than PABP mRNA in heart and skeletal muscle tissue [13].
  • Although the inhibition of the GSPT-PABP interaction did not affect the de novo formation of an 80 S ribosomal initiation complex, it appears to suppress the subsequent recycle of ribosome [14].
  • Amplifications of specific DNA fragments from a human-rodent somatic cell hybrid panel have allowed us to associate PABP1 and PABP3 with 8q22 and 13q11-q12, respectively [15].
  • In this work, we demonstrate that the expression of the amino-terminal one-third of eIF4G, which interacts with eIF4E and PABP, in Xenopus oocyte inhibits translation and progesterone-induced maturation [16].
  • Expression of 3Cpro in HeLa cells resulted in partial PABP cleavage and similar inhibition of translation [2].

Associations of PABPC1 with chemical compounds

  • The important aspect of this mechanism is that PABP binds to an adenosine-rich cis-element at the 5'-untranslated region of its own mRNA and inhibits its translation [17].
  • To study the mechanism of the Paip1-PABP interaction, far-Western, glutathione S-transferase pull-down, and surface plasmon resonance experiments were performed [18].
  • Binding of PABP to the RNA 5' end required the presence of the cap and was accentuated by the N7 methyl moiety of the cap [19].
  • The disease is caused by the expansion of a 10-alanine stretch to 12-17 alanine residues in the poly(A)-binding protein, nuclear 1 (PABPN1; PABP2) [20].
  • In mammalian cells, poly(A) binding protein C1 (PABP C1) has well-known roles in mRNA translation and decay in the cytoplasm [9].

Physical interactions of PABPC1

  • PABP-interacting protein 1 (Paip1) is another factor that interacts with PABP to coactivate translation [21].
  • In mammals, however, there has been no evidence that eIF4G binds PABP [10].
  • The 29 amino acid stretch is almost identical in eIF4GI and eIF4GII, and the full-length eIF4GII also binds PABP [10].
  • Biacore data and far-Western analysis revealed that Paip2 contains two binding sites for PABP, one encompassing a 16-amino-acid stretch located in the C terminus and a second encompassing a larger central region [22].
  • Search for other GSPT-binding proteins in yeast two-hybrid screening system resulted in the identification of a cDNA encoding polyadenylate-binding protein (PABP), whose amino terminus is associating with the poly(A) tail of mRNAs presumably for their stabilization [23].

Regulatory relationships of PABPC1


Other interactions of PABPC1


Analytical, diagnostic and therapeutic context of PABPC1

  • Co-immunoprecipitation experiments showed that the extended eIF4GI binds PABP, while the N-terminally truncated original eIF4GI cannot [10].
  • We raised monoclonal antibodies against the 70-kDa hPABP and confocal immunofluorescence microscopy with these antibodies reveals that it is localized exclusively to the cytoplasm [29].
  • The hPABP exhibits a very low turnover rate in these cells and quantitative immunoblotting experiments demonstrated that growing HeLa cells contain a surprisingly high number of approximately 8 x 10(6) PABP molecules per cell, which corresponds to an intracellular concentration of about 4 microM [29].
  • Phosphorylation by MK2 was confirmed using PABP1 purified by affinity chromatography on poly(A) RNA [30].
  • We have studied the intracellular localization of poly(A)-binding protein 1 (PABP1) by indirect immunofluorescence as well as by tagging with the green fluorescent protein (GFP) in living cells [31].


  1. Expression and prognostic roles of PABPC1 in esophageal cancer: correlation with tumor progression and postoperative survival. Takashima, N., Ishiguro, H., Kuwabara, Y., Kimura, M., Haruki, N., Ando, T., Kurehara, H., Sugito, N., Mori, R., Fujii, Y. Oncol. Rep. (2006) [Pubmed]
  2. Cleavage of poly(A)-binding protein by poliovirus 3C protease inhibits host cell translation: a novel mechanism for host translation shutoff. Kuyumcu-Martinez, N.M., Van Eden, M.E., Younan, P., Lloyd, R.E. Mol. Cell. Biol. (2004) [Pubmed]
  3. Cleavage of Poly(A)-binding protein by coxsackievirus 2A protease in vitro and in vivo: another mechanism for host protein synthesis shutoff? Kerekatte, V., Keiper, B.D., Badorff, C., Cai, A., Knowlton, K.U., Rhoads, R.E. J. Virol. (1999) [Pubmed]
  4. Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer. Deo, R.C., Groft, C.M., Rajashankar, K.R., Burley, S.K. Cell (2002) [Pubmed]
  5. A polyadenylate binding protein localized to the granules of cytolytic lymphocytes induces DNA fragmentation in target cells. Tian, Q., Streuli, M., Saito, H., Schlossman, S.F., Anderson, P. Cell (1991) [Pubmed]
  6. Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation. Craig, A.W., Haghighat, A., Yu, A.T., Sonenberg, N. Nature (1998) [Pubmed]
  7. Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation. Padmanabhan, K., Richter, J.D. Genes Dev. (2006) [Pubmed]
  8. Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms. Kahvejian, A., Svitkin, Y.V., Sukarieh, R., M'Boutchou, M.N., Sonenberg, N. Genes Dev. (2005) [Pubmed]
  9. Evidence that poly(A) binding protein C1 binds nuclear pre-mRNA poly(A) tails. Hosoda, N., Lejeune, F., Maquat, L.E. Mol. Cell. Biol. (2006) [Pubmed]
  10. A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation. Imataka, H., Gradi, A., Sonenberg, N. EMBO J. (1998) [Pubmed]
  11. Structure and function of the C-terminal PABC domain of human poly(A)-binding protein. Kozlov, G., Trempe, J.F., Khaleghpour, K., Kahvejian, A., Ekiel, I., Gehring, K. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  12. X-ray structure of the human hyperplastic discs protein: an ortholog of the C-terminal domain of poly(A)-binding protein. Deo, R.C., Sonenberg, N., Burley, S.K. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  13. iPABP, an inducible poly(A)-binding protein detected in activated human T cells. Yang, H., Duckett, C.S., Lindsten, T. Mol. Cell. Biol. (1995) [Pubmed]
  14. A novel role of the mammalian GSPT/eRF3 associating with poly(A)-binding protein in Cap/Poly(A)-dependent translation. Uchida, N., Hoshino, S., Imataka, H., Sonenberg, N., Katada, T. J. Biol. Chem. (2002) [Pubmed]
  15. Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes. Féral, C., Mattéi, M.G., Pawlak, A., Guellaën, G. Hum. Genet. (1999) [Pubmed]
  16. Inhibition of translation and progesterone-induced maturation of Xenopus oocytes by expressing the amino-terminal portion of the eukaryotic translation initiation factor 4G. Wakiyama, M., Miura, K. Biosci. Biotechnol. Biochem. (2002) [Pubmed]
  17. Reduced stability of mitogen-activated protein kinase kinase-2 mRNA and phosphorylation of poly(A)-binding protein (PABP) in cells overexpressing PABP. Ma, S., Musa, T., Bag, J. J. Biol. Chem. (2006) [Pubmed]
  18. Paip1 interacts with poly(A) binding protein through two independent binding motifs. Roy, G., De Crescenzo, G., Khaleghpour, K., Kahvejian, A., O'Connor-McCourt, M., Sonenberg, N. Mol. Cell. Biol. (2002) [Pubmed]
  19. Poly(A)-binding-protein-mediated regulation of hDcp2 decapping in vitro. Khanna, R., Kiledjian, M. EMBO J. (2004) [Pubmed]
  20. Myopathy phenotype in transgenic mice expressing mutated PABPN1 as a model of oculopharyngeal muscular dystrophy. Hino, H., Araki, K., Uyama, E., Takeya, M., Araki, M., Yoshinobu, K., Miike, K., Kawazoe, Y., Maeda, Y., Uchino, M., Yamamura, K. Hum. Mol. Genet. (2004) [Pubmed]
  21. Translational repression by a novel partner of human poly(A) binding protein, Paip2. Khaleghpour, K., Svitkin, Y.V., Craig, A.W., DeMaria, C.T., Deo, R.C., Burley, S.K., Sonenberg, N. Mol. Cell (2001) [Pubmed]
  22. Dual interactions of the translational repressor Paip2 with poly(A) binding protein. Khaleghpour, K., Kahvejian, A., De Crescenzo, G., Roy, G., Svitkin, Y.V., Imataka, H., O'Connor-McCourt, M., Sonenberg, N. Mol. Cell. Biol. (2001) [Pubmed]
  23. The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein. Hoshino, S., Imai, M., Kobayashi, T., Uchida, N., Katada, T. J. Biol. Chem. (1999) [Pubmed]
  24. mRNA with a <20-nt poly(A) tail imparted by the poly(A)-limiting element is translated as efficiently in vivo as long poly(A) mRNA. Peng, J., Schoenberg, D.R. RNA (2005) [Pubmed]
  25. Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation. Cao, Q., Richter, J.D. EMBO J. (2002) [Pubmed]
  26. Calicivirus 3C-like proteinase inhibits cellular translation by cleavage of poly(A)-binding protein. Kuyumcu-Martinez, M., Belliot, G., Sosnovtsev, S.V., Chang, K.O., Green, K.Y., Lloyd, R.E. J. Virol. (2004) [Pubmed]
  27. In vivo studies of translational repression mediated by the granulocyte-macrophage colony-stimulating factor AU-rich element. Grosset, C., Boniface, R., Duchez, P., Solanilla, A., Cosson, B., Ripoche, J. J. Biol. Chem. (2004) [Pubmed]
  28. Interaction of anti-proliferative protein Tob with poly(A)-binding protein and inducible poly(A)-binding protein: implication of Tob in translational control. Okochi, K., Suzuki, T., Inoue, J., Matsuda, S., Yamamoto, T. Genes Cells (2005) [Pubmed]
  29. The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity. Görlach, M., Burd, C.G., Dreyfuss, G. Exp. Cell Res. (1994) [Pubmed]
  30. Affinity purification of ARE-binding proteins identifies polyA-binding protein 1 as a potential substrate in MK2-induced mRNA stabilization. Bollig, F., Winzen, R., Gaestel, M., Kostka, S., Resch, K., Holtmann, H. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  31. The human poly(A)-binding protein 1 shuttles between the nucleus and the cytoplasm. Afonina, E., Stauber, R., Pavlakis, G.N. J. Biol. Chem. (1998) [Pubmed]
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