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Gene Review

tetR  -  similar to protein family HMM PF02909

Escherichia coli

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Disease relevance of tetR


High impact information on tetR


Chemical compound and disease context of tetR

  • The interaction between E. coli RNA polymerase and the tetR promoter from pSC101, was studied by protection and premodification experiments, using dimethyl sulfate, methylation of single stranded cytosines, and DNAase I footprinting [1].
  • Clinical isolates of E. coli were examined successfully for tetracycline resistance and for the presence of tetR [11].
  • By transiently expressing a chimeric receptor containing Escherichia coli tetracycline repressor (TetR) and LXRbeta LBD and a reporter with a TetR binding site, we show that mutant F268A lost the ability to activate transcription of the reporter, whereas mutant T272A still has an activity similar to that of the wild-type LXRbeta [12].

Biological context of tetR


Anatomical context of tetR


Associations of tetR with chemical compounds

  • The Tn10 tetR gene encodes the repressor that regulates transcription of the Tn10 tetracycline resistance determinant [13].
  • All of these Atr mutations are located in the Tn10 tet region; the majority (18 of 20) have no effect on tetR repressor function [17].
  • An example is given in which a galK gene, lacking the normal initiator methionine codon, is fused to various segments of the 5' end of the tetR gene of pBR322 [18].

Other interactions of tetR

  • Subcloning, transposon mutagenesis, and DNA sequence analysis revealed that this DNA fragment contained two divergently transcribed genes, tetA and tetR, encoding products that were very similar to proteins of the Tet(A) class of tetracycline resistance systems [19].
  • In addition, it appears that E. coli cannot tolerate constitutive expression of the wild-type tetA gene from a multicopy plasmid containing a tetR deletion [17].
  • Tetracycline in the medium induces divergent transcription of the tetA and tetR genes within the transposon, and this transcription extends beyond the transposon in both directions into the bacterial genes [20].
  • The structural genes for tetA, the Tn10 tetracycline-resistance function, and for tetR, the Tn10 tet repressor, are transcribed in opposite directions from promoters in a regulatory region located between the two structural genes [21].

Analytical, diagnostic and therapeutic context of tetR


  1. Interaction between E. coli RNA polymerase and the tetR promoter from pSC101: homologies and differences with other E. coli promoter systems from close contact point studies. Duval-Valentin, G., Ehrlich, R. Nucleic Acids Res. (1986) [Pubmed]
  2. Conditional transformation of a pancreatic beta-cell line derived from transgenic mice expressing a tetracycline-regulated oncogene. Efrat, S., Fusco-DeMane, D., Lemberg, H., al Emran, O., Wang, X. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  3. Ecological impact of antibiotic use in animals on different complex microflora: environment. Witte, W. Int. J. Antimicrob. Agents (2000) [Pubmed]
  4. The use of beta-galactosidase gene fusions to screen for antibacterial antibiotics. Kirsch, D.R., Lai, M.H., McCullough, J., Gillum, A.M. J. Antibiot. (1991) [Pubmed]
  5. Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus. Kim, H.J., Gatz, C., Hillen, W., Jones, T.R. J. Virol. (1995) [Pubmed]
  6. Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Wirtz, E., Clayton, C. Science (1995) [Pubmed]
  7. Conditional ectopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis. Wu, Z., Xie, Y., Bucher, N.L., Farmer, S.R. Genes Dev. (1995) [Pubmed]
  8. Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2. Meier, I., Wray, L.V., Hillen, W. EMBO J. (1988) [Pubmed]
  9. Construction of an E. coli strain overproducing the Tn10-encoded TET repressor and its use for large scale purification. Oehmichen, R., Klock, G., Altschmied, L., Hillen, W. EMBO J. (1984) [Pubmed]
  10. Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Urlinger, S., Baron, U., Thellmann, M., Hasan, M.T., Bujard, H., Hillen, W. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  11. A quantitative real-time PCR assay for the detection of tetR of Tn10 in Escherichia coli using SYBR Green and the Opticon. Morsczeck, C., Langendörfer, D., Schierholz, J.M. J. Biochem. Biophys. Methods (2004) [Pubmed]
  12. The important role of residue F268 in ligand binding by LXRbeta. Urban, F., Cavazos, G., Dunbar, J., Tan, B., Escher, P., Tafuri, S., Wang, M. FEBS Lett. (2000) [Pubmed]
  13. Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant. Postle, K., Nguyen, T.T., Bertrand, K.P. Nucleic Acids Res. (1984) [Pubmed]
  14. Supercoil-induced unusual DNA structures as transcriptional block. Bagga, R., Ramesh, N., Brahmachari, S.K. Nucleic Acids Res. (1990) [Pubmed]
  15. Detection of tetracyclines and efflux pump inhibitors. Rothstein, D.M., McGlynn, M., Bernan, V., McGahren, J., Zaccardi, J., Cekleniak, N., Bertrand, K.P. Antimicrob. Agents Chemother. (1993) [Pubmed]
  16. A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. Beck, C.F., Mutzel, R., Barbé, J., Müller, W. J. Bacteriol. (1982) [Pubmed]
  17. Mutations in multicopy Tn10 tet plasmids that confer resistance to inhibitory effects of inducers of tet gene expression. Moyed, H.S., Bertrand, K.P. J. Bacteriol. (1983) [Pubmed]
  18. Expression of galactokinase as a fusion protein in Escherichia coli and Saccharomyces cerevisiae. Heusterspreute, M., Ha Thi, V., Davison, J. DNA (1984) [Pubmed]
  19. Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58. Luo, Z.Q., Farrand, S.K. J. Bacteriol. (1999) [Pubmed]
  20. Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon. Takiff, H.E., Baker, T., Copeland, T., Chen, S.M., Court, D.L. J. Bacteriol. (1992) [Pubmed]
  21. Overlapping divergent promoters control expression of Tn10 tetracycline resistance. Bertrand, K.P., Postle, K., Wray, L.V., Reznikoff, W.S. Gene (1983) [Pubmed]
  22. A differential scanning calorimetry study of tetracycline repressor. Kedracka-Krok, S., Wasylewski, Z. Eur. J. Biochem. (2003) [Pubmed]
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