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Gene Review

Krt5  -  keratin 5

Rattus norvegicus

Synonyms: CK-5, Cytokeratin-5, K5, Keratin, type II cytoskeletal 5, Keratin-5, ...
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Disease relevance of Krt2-5

  • However, differential expression of genes, especially cytokeratins (CK8 and CK5) and collagens (III and IV) between highly metastatic and low metastatic rat mammary adenocarcinoma cell lines might initiate further investigation of these genes in metastatic process [1].

High impact information on Krt2-5

  • Rat keratin K5 and vimentin complementary DNAs have been isolated, identified, and used to study keratin and vimentin expression as markers for cell differentiation [2].
  • Herein, we demonstrate that mouse and rat spermatids assemble in the subacrosomal space a cytoskeletal scaffold containing F-actin and Sak57, a keratin ortholog [3].
  • K5 reduced vascular permeability at doses substantially lower than that required for inhibition of retinal neovascularisation [4].
  • Previously we have shown that plasminogen kringle 5 (K5), an angiogenic inhibitor, inhibits retinal neovascularisation in the rat model of oxygen-induced retinopathy (OIR) [4].
  • Moreover, K5 inhibited IGF-1-induced hyperpermeability, which is known to arise through up-regulation of endogenous VEGF expression [4].

Biological context of Krt2-5


Anatomical context of Krt2-5

  • Gap junctions in undifferentiated epithelial cells at days 1-10 of life were composed of Cx 43, which always colocalized with basal cell cytokeratins (CK 5/15) [9].
  • Confocal laser scanning microscopy was used to analyze the three-dimensional arrangement of Sak57 in pachytene spermatocytes [10].
  • Rat sperm tail samples displayed only the Sak57 detergent-insoluble form and its pl was more acidic (4.7-4.8) [10].
  • Immunogold electron microscopy confirmed the presence of Sak57 immunoreactivity among microtubules of the manchette and on outer dense fibers and the longitudinal columns linking the ribs of the fibrous sheath [8].
  • We have found that rat testis displays two immunoreactive proteins: a soluble 83 kDa protein with pl range 5.9-6.3, regarded as a precursor, and both detergent-insoluble and soluble 57 kDa protein with pl range 5.0-5.9, corresponding to the mature form Sak57 [10].

Associations of Krt2-5 with chemical compounds

  • RESULTS: Two types of cell populations, a large single-cell population and a small single-cell population, were obtained and characterized as basal and luminal epithelial cells by immunostaining for cytokeratin 5 and cytokeratin 8, respectively [11].
  • Anti-MAP sera were used for further analysis of the biochemical characteristics of Sak57 in testis and sperm tails using chromatofocusing, immunoblotting, and [32P] orthophosphate-labeling [10].
  • We have purified a 57 kDa protein (designated Sak57, for spermatogenic cell/sperm-associated keratin) from sodium dodecyl sulfate-beta-mercaptoethanol (SDS-beta ME)-dissociated outer dense fibers isolated from rat sperm tails [10].
  • RESULTS: Subcutaneous, intraperitoneal, subconjunctival, and retrobulbar injections and topical eyedrop application of K5 significantly reduced retinal vascular permeability in both the OIR and STZ-diabetic rat models [12].

Other interactions of Krt2-5


Analytical, diagnostic and therapeutic context of Krt2-5


  1. Identification of differentially expressed genes in isogenic highly metastatic and poorly metastatic cell lines of R3230AC rat mammary adenocarcinoma. Günes, H., Carlsen, S.A. Cell Prolif. (2003) [Pubmed]
  2. Loss of keratin expression in anaplastic carcinoma cells due to posttranscriptional down-regulation acting in trans. Paine, M.L., Gibbins, J.R., Chew, K.E., Demetriou, A., Kefford, R.F. Cancer Res. (1992) [Pubmed]
  3. Acroplaxome, an F-actin-keratin-containing plate, anchors the acrosome to the nucleus during shaping of the spermatid head. Kierszenbaum, A.L., Rivkin, E., Tres, L.L. Mol. Biol. Cell (2003) [Pubmed]
  4. Plasminogen kringle 5 reduces vascular leakage in the retina in rat models of oxygen-induced retinopathy and diabetes. Zhang, S.X., Sima, J., Shao, C., Fant, J., Chen, Y., Rohrer, B., Gao, G., Ma, J.X. Diabetologia (2004) [Pubmed]
  5. Transdifferentiation of NRP-152 rat prostatic basal epithelial cells toward a luminal phenotype: regulation by glucocorticoid, insulin-like growth factor-I and transforming growth factor-beta. Danielpour, D. J. Cell. Sci. (1999) [Pubmed]
  6. Polyomavirus middle T selective action on cytokeratin 14 gene expression in liver nonparenchymal epithelial cells. Royal, I., Grenier, A., Mailhot, D., Marceau, N. Exp. Cell Res. (1995) [Pubmed]
  7. Sak 57, an intermediate filament keratin present in intercellular bridges of rat primary spermatocytes. Tres, L.L., Rivkin, E., Kierszenbaum, A.L. Mol. Reprod. Dev. (1996) [Pubmed]
  8. Sak57, an acidic keratin initially present in the spermatid manchette before becoming a component of paraaxonemal structures of the developing tail. Tres, L.L., Kierszenbaum, A.L. Mol. Reprod. Dev. (1996) [Pubmed]
  9. Developmental exposure to estrogens alters epithelial cell adhesion and gap junction proteins in the adult rat prostate. Habermann, H., Chang, W.Y., Birch, L., Mehta, P., Prins, G.S. Endocrinology (2001) [Pubmed]
  10. Purification, partial characterization, and localization of Sak57, an acidic intermediate filament keratin present in rat spermatocytes, spermatids, and sperm. Kierszenbaum, A.L., Rivkin, E., Fefer-Sadler, S., Mertz, J.R., Tres, L.L. Mol. Reprod. Dev. (1996) [Pubmed]
  11. Isolation of rat ventral prostate basal and luminal epithelial cells by the STAPUT technique. Ravindranath, N., Dym, M. Prostate (1999) [Pubmed]
  12. Systemic and periocular deliveries of plasminogen kringle 5 reduce vascular leakage in rat models of oxygen-induced retinopathy and diabetes. Zhang, S.X., Sima, J., Wang, J.J., Shao, C., Fant, J., Ma, J.X. Curr. Eye Res. (2005) [Pubmed]
  13. Epithelial development in the rat ventral prostate, anterior prostate and seminal vesicle. Hayward, S.W., Baskin, L.S., Haughney, P.C., Cunha, A.R., Foster, B.A., Dahiya, R., Prins, G.S., Cunha, G.R. Acta anatomica. (1996) [Pubmed]
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